CAZyme Information: MGYG000000209_02813

Basic Information

• Species: Eubacterium_F sp003491505
• Lineage: Bacteria; Firmicutes_A; Clostridia; Lachnospirales; Lachnospiraceae; Eubacterium_F; Eubacterium_F sp003491505
• CAZyme ID: MGYG000000209_02813
• CAZy Family: GH146
• CAZyme Description: hypothetical protein

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
671		76186.24	4.9549

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000000209	3107681	Isolate	China	Asia

• Gene Location: Start: 664; End: 2679 Strand: +

Full Sequence      

MNQDISICVLTENEMGWTEPFELDKVQILDNYYLSAQKSDIAFLKKMDTARLLAGFRTTA
GIDTKGVRPYGGWEDSLLGGHCVGHYLTALAQAVKVTGDKELKEKSQTLIAGLEECQKKL
GTGFLFGAKVEDKEDVEKQFDILEGKKKGETWVPWYNMHKVLAGLVDTYKYTGNETALLV
AEKLGDWIYERVSKWDLKTNQKVLETEYGGMNDCLYELYSHSHNKHHLEAAQKFDEKALF
LMAAKGEKNCLDGKHANTQIPKFIGAIKRYNVLKQLGEAKQEDEAYLVDAEKFFEMVVKR
HSFVTGGISVMEHFRKDYHLDEIRTQTNCESCCAHNMLKLAKELFKATRKKEYADYYETT
LRNAIMGAVKTESGAASYFTPMATGYYKTFGEEEPEKNMFWCCTGSGMENFTKLGDSIYF
RANDTLLVNQYVASKVTWEEKNLVLTQKSDVTKSEEISFVLNALHDKEISDVAIALRIPD
WMHGEATIYVNGAEKMTAAGNSEYVLLERNWEDGDVIMAKYPMSVESVGLLDQDAVFAFR
YGPTVLAAKLGKEKMGEATWAGIDLTAPLYKVVGNECRKDTIAYGEPKTTELLDNETLTI
QKETSVNEFVSHIERYLVRDTESETLSFHLKGTDADTTFENGLQFVPFNTLNDERYGIYW
YFDTEKNNDKE

Enzyme Prediction     

No EC number prediction in MGYG000000209_02813.

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GH146	26	547	1.7e-170	0.9960238568588469

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
pfam07944	Glyco_hydro_127	4.03e-134	26	547	2	503	Beta-L-arabinofuranosidase, GH127. One member of this family, from Bidobacterium longicum, UniProtKB:E8MGH8, has been characterized as an unusual beta-L-arabinofuranosidase enzyme, EC:3.2.1.185. It rleases l-arabinose from the l-arabinofuranose (Araf)-beta1,2-Araf disaccharide and also transglycosylates 1-alkanols with retention of the anomeric configuration. Terminal beta-l-arabinofuranosyl residues have been found in arabinogalactan proteins from a mumber of different plantt species. beta-l-Arabinofuranosyl linkages with 1-4 arabinofuranosides are also found in the sugar chains of extensin and solanaceous lectins, hydroxyproline (Hyp)2-rich glycoproteins that are widely observed in plant cell wall fractions. The critical residue for catalytic activity is Glu-338, in a ET/SCAS sequence context.
COG3533	COG3533	1.95e-77	36	560	22	514	Uncharacterized conserved protein, DUF1680 family [Function unknown].
pfam16375	DUF4986	3.73e-04	618	660	43	84	Domain of unknown function. This family around 150 residues locates in the C-terminal of some uncharacterized proteins in various Bacteroides and Bacillus species. The function of this family remains unknown.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
ADL33431.1	3.47e-164	21	666	5	617
CBL17203.1	4.31e-159	19	662	37	655
AEY65173.1	3.87e-156	21	664	38	642
ACL75592.1	8.30e-156	21	664	38	642
ABG76968.1	8.30e-156	21	664	38	642

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
6YQH_AAA	1.67e-88	21	660	28	648	ChainAAA, Acetyl-CoA carboxylase, biotin carboxylase [Bacteroides thetaiotaomicron VPI-5482]
5OPJ_A	6.54e-84	21	660	28	648	Beta-L-arabinofuranosidase[Bacteroides thetaiotaomicron]
4QJY_A	3.02e-13	148	528	180	537	Crystalstructure of native Ara127N, a GH127 beta-L-arabinofuranosidase from Geobacillus Stearothermophilus T6 [Geobacillus stearothermophilus],4QJY_B Crystal structure of native Ara127N, a GH127 beta-L-arabinofuranosidase from Geobacillus Stearothermophilus T6 [Geobacillus stearothermophilus]
3WRE_A	2.33e-10	283	528	290	543	Thecrystal structure of native HypBA1 from Bifidobacterium longum JCM 1217 [Bifidobacterium longum subsp. longum JCM 1217],3WRG_A The complex structure of HypBA1 with L-arabinose [Bifidobacterium longum subsp. longum JCM 1217]
3WKW_A	2.35e-10	283	528	290	543	Crystalstructure of GH127 beta-L-arabinofuranosidase HypBA1 from Bifidobacterium longum ligand free form [Bifidobacterium longum subsp. longum JCM 1217],3WKX_A Crystal structure of GH127 beta-L-arabinofuranosidase HypBA1 from Bifidobacterium longum arabinose complex form [Bifidobacterium longum subsp. longum JCM 1217],7BZL_A Chain A, Non-reducing end beta-L-arabinofuranosidase [Bifidobacterium longum subsp. longum JCM 1217],7DIF_A Chain A, Non-reducing end beta-L-arabinofuranosidase [Bifidobacterium longum subsp. longum JCM 1217],7EXV_A Chain A, Non-reducing end beta-L-arabinofuranosidase [Bifidobacterium longum subsp. longum JCM 1217],7EXW_A Chain A, Non-reducing end beta-L-arabinofuranosidase [Bifidobacterium longum subsp. longum JCM 1217]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
E8MGH8	1.28e-09	283	528	290	543	Non-reducing end beta-L-arabinofuranosidase OS=Bifidobacterium longum subsp. longum (strain ATCC 15707 / DSM 20219 / JCM 1217 / NCTC 11818 / E194b) OX=565042 GN=hypBA1 PE=1 SV=1

SignalP and Lipop Annotations

This protein is predicted as OTHER

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
1.000061	0.000000	0.000000	0.000000	0.000000	0.000000

TMHMM  Annotations     

There is no transmembrane helices in MGYG000000209_02813.