CAZyme Information: MGYG000000211_04459

Basic Information

• Species: Bacteroides sp900556215
• Lineage: Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Bacteroidaceae; Bacteroides; Bacteroides sp900556215
• CAZyme ID: MGYG000000211_04459
• CAZy Family: PL8
• CAZyme Description: hypothetical protein

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
1589	MGYG000000211_11|CGC3	179114.14	8.1698

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000000211	6486320	Isolate	China	Asia

• Gene Location: Start: 138994; End: 143763 Strand: -

Full Sequence      

MFMKCLKLIFCLLYLCCSLAEAKEVQVDAQKEVDEMTGRLVASYLETKVDEDIIAGYASA
LRSDGSFPDLDYVTVHEGSFYPAGSHLKRLKSMAIAYRKPGNKYYNSGKLLKQIVAGIDY
WYKVRPKSRNWWFGDIGAPQDYMVPLILLKGKISEKKLLHYSSYLQDLTGNKGHKGKNRT
WVSAVTIHKGCIEDNIELIRTGIESIASTIKIVPEQGDEGIKVDGSFHQHRPQLYSGGYG
LSYVDDIAYYLRLVKGTVFESYFTQEKKEIFINLLMGGHRLLGYRETFDFGAIGRNISRS
EGLSNISPVTLEYMEQNDPERVADYSAWKKHLSGAPFPAPGNKYFWKSDIMVQHGAGYYL
SAKVISTRTNGTEMLNNENLKGYNLPLGATNILTSGKEYGGIFPIWNWNKIPGTTAIQHP
DSARLEGYLFGKNRYGGGVSNGKNGVIAYEHCYKGVKAKKAYFFMNDVLLCLGTDIASNA
PEEVVTTVNQCLFKGEMVVGKEEGTTSVYKENASAKNPAWVYHDKVGYLFPSGGDVTVSS
PKQTGAWKDINISGSGKKISADIFNLWISHGVKAKEGKYAYMVVPDRSLKEFQTFAATQN
YKIIQNSSAVQAVRVNHQYAVVFYHPGIIDLGEGLTLAADKQVIVYLEQKGTDYDIWVAD
PLYSQKRVCLTLNGREVQIAFPGDELTGSTAFTNIATLQPFDLRCEYLSNPLGIDIQQPR
LSWKMGATVPARGRRQTAYQILVASSRELLDADRGDLWDSGRVNSAESVNIVYEGVPLSA
GKRCFWKVRFSDEHNHWSAWSTTANWRMGLFAADWSARWIGSAEMESQSVGGKKVNNVMA
DPWFRKTFELSEISQDAIIYVASIGYHELYVNGRKVGDAVLSPSVTDHKSRARYMTYDIR
NYLKKGTNVIALWLGTSWAVFPAYQQKDRPAIPMALAQAEITLSSGKKLRIVSDNTWKTH
ASPNTLLGYWEAHHFEGECYDAALEKDGWNTPEFDDSGWAMAKAYSTDVTVSSDRTEPNR
LLDEIKPLSVQEISPGVYRVDMGINYAGWFEMQLQGQPGDSIVFQFSEREKDACSYGIHS
IYKVGPKRKGTFCNRFNYMTGRWIQITGLRYKPQLDQIRGWMIRPDYCRSGEFECDVPLL
NDIYRTTLWTFENLSLGNYVVDCPHRERCGYGGDALATTRTALGNYQLGAFYNKWMEDWR
DVQDPEGNVPYTAPTRIGGGGPSWSGFCITLPWEFYRQYGDVRILSESFPTIQRWLSFVE
TKSQNDMLVRWGGKWSFLGDWLWPDAWPERSAMEKQGKALGDTRETLFFNNCHWIYSLET
AAQIADVLGNEKAASTYRNRASKVRKAVHATFFNSKDNSYVNGYPSYLAIALMVDLPPKH
LKAKVWKRLEQEIQVKRKGHFWGGITAGSFLLHTLLDNHRNDLIFEMATKEDFPGWGDML
KHGNGTFFEDWQCRGSGLHSSYLYIGSWFIEALGGIRRPEAGYKEFTIEPWITEKGPQQV
RSHYNSMYGNIVSNWAVNSGVLNLEVTVPANTTAILKLSDIDLGTLKEGDSIWKEAKGVS
LYMQKGKTISLALQSGTYRFSVTMNSSVQ

Enzyme Prediction     

No EC number prediction in MGYG000000211_04459.

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GH78	1031	1538	5.4e-122	0.996031746031746
PL8	343	586	6.3e-80	0.9879518072289156
CBM67	817	1005	1.5e-34	0.9488636363636364

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
cd01083	GAG_Lyase	2.89e-122	31	661	8	693	Glycosaminoglycan (GAG) polysaccharide lyase family. This family consists of a group of secreted bacterial lyase enzymes capable of acting on glycosaminoglycans, such as hyaluronan and chondroitin, in the extracellular matrix of host tissues, contributing to the invasive capacity of the pathogen. These are broad-specificity glycosaminoglycan lyases which recognize uronyl residues in polysaccharides and cleave their glycosidic bonds via a beta-elimination reaction to form a double bond between C-4 and C-5 of the non-reducing terminal uronyl residues of released products. Substrates include chondroitin, chondroitin 4-sulfate, chondroitin 6-sulfate, and hyaluronic acid. Family members include chondroitin AC lyase, chondroitin abc lyase, xanthan lyase, and hyalurate lyase.
pfam02278	Lyase_8	4.98e-86	342	588	1	252	Polysaccharide lyase family 8, super-sandwich domain. This family consists of a group of secreted bacterial lyase enzymes EC:4.2.2.1 capable of acting on hyaluronan and chondroitin in the extracellular matrix of host tissues, contributing to the invasive capacity of the pathogen.
pfam17389	Bac_rhamnosid6H	2.38e-70	1129	1473	2	340	Bacterial alpha-L-rhamnosidase 6 hairpin glycosidase domain. This family consists of bacterial rhamnosidase A and B enzymes. L-Rhamnose is abundant in biomass as a common constituent of glycolipids and glycosides, such as plant pigments, pectic polysaccharides, gums or biosurfactants. Some rhamnosides are important bioactive compounds. For example, terpenyl glycosides, the glycosidic precursor of aromatic terpenoids, act as important flavouring substances in grapes. Other rhamnosides act as cytotoxic rhamnosylated terpenoids, as signal substances in plants or play a role in the antigenicity of pathogenic bacteria.
pfam08531	Bac_rhamnosid_N	3.65e-40	856	1020	5	169	Alpha-L-rhamnosidase N-terminal domain. This family consists of bacterial rhamnosidase A and B enzymes. This domain is probably involved in substrate recognition.
pfam08124	Lyase_8_N	2.72e-31	48	304	27	291	Polysaccharide lyase family 8, N terminal alpha-helical domain. This family consists of a group of secreted bacterial lyase enzymes EC:4.2.2.1 capable of acting on hyaluronan and chondroitin in the extracellular matrix of host tissues, contributing to the invasive capacity of the pathogen.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
ALJ60391.1	0.0	1	1585	1	1585
QUT88602.1	0.0	3	1585	1	1583
QUT74996.1	0.0	698	1585	33	920
BCI64727.1	0.0	702	1585	42	925
QDU88571.1	7.02e-291	703	1584	798	1689

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
6I60_A	2.27e-114	700	1580	32	939	Structureof alpha-L-rhamnosidase from Dictyoglumus thermophilum [Dictyoglomus thermophilum H-6-12],6I60_B Structure of alpha-L-rhamnosidase from Dictyoglumus thermophilum [Dictyoglomus thermophilum H-6-12]
3W5M_A	3.73e-92	706	1534	12	990	CrystalStructure of Streptomyces avermitilis alpha-L-rhamnosidase [Streptomyces avermitilis MA-4680 = NBRC 14893],3W5N_A Crystal Structure of Streptomyces avermitilis alpha-L-rhamnosidase complexed with L-rhamnose [Streptomyces avermitilis MA-4680 = NBRC 14893]
1CB8_A	1.38e-84	59	690	33	674	CHONDROITINASEAC LYASE FROM FLAVOBACTERIUM HEPARINUM [Pedobacter heparinus]
1HM2_A	2.28e-84	59	690	55	696	ACTIVESITE OF CHONDROITINASE AC LYASE REVEALED BY THE STRUCTURE OF ENZYME-OLIGOSACCHARIDE COMPLEXES AND MUTAGENESIS [Pedobacter heparinus],1HM3_A Active Site Of Chondroitinase Ac Lyase Revealed By The Structure Of Enzyme-Oligosaccharide Complexes And Mutagenesis [Pedobacter heparinus],1HMU_A ACTIVE SITE OF CHONDROITINASE AC LYASE REVEALED BY THE STRUCTURE OF ENZYME-OLIGOSACCHARIDE COMPLEXES AND MUTAGENESIS [Pedobacter heparinus],1HMW_A Active Site Of Chondroitinase Ac Lyase Revealed By The Structure Of Enzyme-oligosaccharide Complexes And Mutagenesis [Pedobacter heparinus]
6GSZ_A	2.34e-69	706	1538	10	846	Crystalstructure of native alfa-L-rhamnosidase from Aspergillus terreus [Aspergillus terreus]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
T2KNB2	5.72e-105	706	1580	42	920	Alpha-L-rhamnosidase OS=Formosa agariphila (strain DSM 15362 / KCTC 12365 / LMG 23005 / KMM 3901 / M-2Alg 35-1) OX=1347342 GN=BN863_22090 PE=1 SV=2
T2KPL4	1.66e-97	709	1579	41	946	Alpha-L-rhamnosidase OS=Formosa agariphila (strain DSM 15362 / KCTC 12365 / LMG 23005 / KMM 3901 / M-2Alg 35-1) OX=1347342 GN=BN863_22170 PE=2 SV=1
Q82PP4	1.66e-91	706	1534	12	990	Alpha-L-rhamnosidase OS=Streptomyces avermitilis (strain ATCC 31267 / DSM 46492 / JCM 5070 / NBRC 14893 / NCIMB 12804 / NRRL 8165 / MA-4680) OX=227882 GN=SAVERM_828 PE=1 SV=1
P9WF03	2.63e-86	706	1534	38	874	Alpha-L-rhamnosidase OS=Alteromonas sp. (strain LOR) OX=1537994 GN=LOR_34 PE=1 SV=1
Q59288	1.25e-83	59	690	55	696	Chondroitinase-AC OS=Pedobacter heparinus (strain ATCC 13125 / DSM 2366 / CIP 104194 / JCM 7457 / NBRC 12017 / NCIMB 9290 / NRRL B-14731 / HIM 762-3) OX=485917 GN=cslA PE=1 SV=1

SignalP and Lipop Annotations

This protein is predicted as SP

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
0.000198	0.999147	0.000199	0.000147	0.000136	0.000130

TMHMM  Annotations     

There is no transmembrane helices in MGYG000000211_04459.