CAZyme Information: MGYG000000211_04633

Basic Information

• Species: Bacteroides sp900556215
• Lineage: Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Bacteroidaceae; Bacteroides; Bacteroides sp900556215
• CAZyme ID: MGYG000000211_04633
• CAZy Family: CBM38
• CAZyme Description: hypothetical protein

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
1021	MGYG000000211_12|CGC3	115296.4	6.0778

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000000211	6486320	Isolate	China	Asia

• Gene Location: Start: 183886; End: 186951 Strand: -

Full Sequence      

MSLSLPSFSQYSPDHKVSLDKGDIKSFIGDKETKGERKVYKGDELTTIGMPVGGICAGQL
YVRGDGTLANWWIANNAYNTGYGIDWLLNFDTPLGPWKVCYQTFEPASYIDQGFKITVDD
GNKTVTKELNKKDFDDIAFIGEYPIAFIEYGQKNENLPVKVSAEVYSPFVPLDAKKSATP
ATIMKYTVKNTSDKKITASLEGWMQNLVCIDLKGRADGMLQNKVIDNNGWKSVYMDMRVD
NLPTKAVDKSRKYEVFENFESGTYNRWKVEGTAFGRKPSPGEQCNQNPFGSDYGNYLANS
YTNYDKPVGKLTSQPFAIKYKFISFNIAGGSHPGKTCINLVIDGKVVMSATGNNNEDFLP
RNWDVSKYKGKKAYLEIVDSVEGDWGHISVDNIEFSNSPVDAPATTIDKSHAYFGNLALS
VFDNNATGTADHSKNNVEYAETELGNKINGALTSSMTLQPGESKEVTFVLSWYFPNRPLS
YKGSEWNKPLPPNSPAIGNMYSNWYNSSLDVAQQLHENYAELAKETHTFHNTYYNQTTLP
YWLVQRVMMSISTLATETCQWWATDKFWAWEGVGSCEGTCTHVWGYAQSMAALFPELERN
LREKTDYSVSLREDGAILSRNGEHGVLIDGHASVILRMYREHLMSKNNFFLSRNWDKIKR
SLQYIINEDGNEDGLIVKTQPNTYDISFNGANTYVGALYLAALRAGEKMAYIMKDTVAAN
YYKKIYTAGSENTMDRLWNGEYFYQDVDEKEYPKYQYGKGCLSDQLFGQTWASLYRLGDI
YPTDAINKALRSIWKFNWTNDVKAQSEAHLPERYYAHAGEPGLFICTWPYSKHPGEDGVR
YRDEVWTGIEYQVATEMIKRDMIDEGLAIVKAIHSRYDGKKHNPWNEIECGDHYARAMAS
YGILQAVQGYWYDGPKGIMGFDPKLRKDDFTGFFNAAEGWGNISQTRKNNKQVNCVEVKF
GRLYLDKLQVNLPAGTGINNLKASLNNQDIAVSAANTNGYTEISLNGLQMRLGDKLQITF
E

Enzyme Prediction     

No EC number prediction in MGYG000000211_04633.

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GH116	628	903	4.4e-65	0.7272727272727273
CBM38	258	380	6.6e-36	0.9612403100775194

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
pfam04685	DUF608	5.48e-45	629	903	99	362	Glycosyl-hydrolase family 116, catalytic region. This represents a family of archaeal, bacterial and eukaryotic glycosyl hydrolases, that belong to superfamily GH116. The primary catabolic pathway for glucosylceramide is catalysis by the lysosomal enzyme glucocerebrosidase. In higher eukaryotes, glucosylceramide is the precursor of glycosphingolipids, a complex group of ubiquitous membrane lipids. Mutations in the human protein cause motor-neurone defects in hereditary spastic paraplegia. The catalytic nucleophile, identified in UniProtKB:Q97YG8_SULSO, is a glutamine-335, with the likely acid/base at Asp-442 and the aspartates at Asp-406 and Asp-458 residues also playing a role in the catalysis of glucosides and xylosides that are beta-bound to hydrophobic groups. The family is defined as GH116, which presently includes enzymes with beta-glucosidase, EC:3.2.1.21, beta-xylosidase, EC:3.2.1.37, and glucocerebrosidase EC:3.2.1.45 activity.
COG4354	COG4354	1.14e-42	40	910	5	720	Uncharacterized protein, contains GBA2_N and DUF608 domains [Function unknown].
pfam12215	Glyco_hydr_116N	2.85e-14	49	522	1	309	beta-glucosidase 2, glycosyl-hydrolase family 116 N-term. This domain is found in bacteria, archaea and eukaryotes. This domain is typically between 320 to 354 amino acids in length. This domain is found associated with pfam04685. It is found just after the extreme N-terminus. The N-terminal is thought to be the luminal domain while the C terminal is the cytosolic domain. The catalytic domain of GBA-2 is unknown. The primary catabolic pathway for glucosylceramide is catalysis by the lysosomal enzyme glucocerebrosidase. In higher eukaryotes, glucosylceramide is the precursor of glycosphingolipids, a complex group of ubiquitous membrane lipids. Mutations in the human protein cause motor-neurone defects in hereditary spastic paraplegia. The catalytic nucleophile, identified in UniProtKB:Q97YG8_SULSO, is a glutamine-335 in the downstream family pfam04685.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
QDO68264.1	0.0	1	1021	13	1033
QUT37401.1	0.0	1	1021	13	1033
QBJ18459.1	0.0	1	1021	13	1033
ASV73565.1	2.39e-267	14	974	13	963
QDV74336.1	8.56e-253	13	976	58	1021

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
5FJS_A	8.62e-26	300	885	143	753	Bacterialbeta-glucosidase reveals the structural and functional basis of genetic defects in human glucocerebrosidase 2 (GBA2) [Thermoanaerobacterium xylanolyticum LX-11],5FJS_B Bacterial beta-glucosidase reveals the structural and functional basis of genetic defects in human glucocerebrosidase 2 (GBA2) [Thermoanaerobacterium xylanolyticum LX-11],5O0S_A Crystal structure of txGH116 (beta-glucosidase from Thermoanaerobacterium xylolyticum) in complex with unreacted beta Cyclophellitol Cyclosulfate probe ME711 [Thermoanaerobacterium xylanolyticum LX-11]
5NPF_A	8.83e-26	300	885	146	756	Crystalstructure of txGH116 (beta-glucosidase from Thermoanaerobacterium xylolyticum) in complex with beta Cyclophellitol Cyclosulfate probe ME594 [Thermoanaerobacterium xylanolyticum]
5BVU_A	8.85e-26	300	885	147	757	Crystalstructure of Thermoanaerobacterium xylolyticum GH116 beta-glucosidase [Thermoanaerobacterium xylanolyticum LX-11],5BX2_A Crystal structure of Thermoanaerobacterium xylanolyticum GH116 beta-glucosidase with 2-deoxy-2-fluoroglucoside [Thermoanaerobacterium xylanolyticum LX-11],5BX3_A Crystal structure of Thermoanaerobacterium xylanolyticum GH116 beta-glucosidase with deoxynojirimycin [Thermoanaerobacterium xylanolyticum LX-11],5BX4_A Crystal structure of Thermoanaerobacterium xylanolyticum GH116 beta-glucosidase with Glucoimidazole [Thermoanaerobacterium xylanolyticum LX-11],5BX5_A Crystal structure of Thermoanaerobacterium xylanolyticum GH116 beta-glucosidase with glucose [Thermoanaerobacterium xylanolyticum LX-11],5NCX_A Crystal structure of Thermoanaerobacterium xylolyticum GH116 beta-glucosidase with an covalent inhibitor [Thermoanaerobacterium xylanolyticum LX-11],5OST_A Beta-glucosidase from Thermoanaerobacterium xylolyticum GH116 in complex with Gluco-1H-imidazole [Thermoanaerobacterium xylanolyticum LX-11]
7W2V_A	9.60e-26	300	885	190	800	ChainA, Glucosylceramidase [Thermoanaerobacterium xylanolyticum LX-11]
7W2W_A	9.60e-26	300	885	190	800	ChainA, Glucosylceramidase [Thermoanaerobacterium xylanolyticum LX-11],7W2X_A Chain A, Glucosylceramidase [Thermoanaerobacterium xylanolyticum LX-11]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
K0E4E1	2.00e-24	253	423	316	487	Putative glycosyl hydrolase ecdE OS=Aspergillus rugulosus OX=41736 GN=ecdE PE=3 SV=1
Q9HCG7	2.08e-11	629	866	619	841	Non-lysosomal glucosylceramidase OS=Homo sapiens OX=9606 GN=GBA2 PE=1 SV=2
Q69ZF3	2.72e-11	629	866	610	832	Non-lysosomal glucosylceramidase OS=Mus musculus OX=10090 GN=Gba2 PE=1 SV=2
Q7KT91	3.63e-11	650	865	687	890	Non-lysosomal glucosylceramidase OS=Drosophila melanogaster OX=7227 GN=CG33090 PE=1 SV=1
Q5M868	4.69e-11	629	866	610	832	Non-lysosomal glucosylceramidase OS=Rattus norvegicus OX=10116 GN=Gba2 PE=2 SV=2

SignalP and Lipop Annotations

This protein is predicted as OTHER

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
1.000054	0.000000	0.000000	0.000000	0.000000	0.000000

TMHMM  Annotations     

There is no transmembrane helices in MGYG000000211_04633.