CAZyme Information: MGYG000000211_04634

Basic Information

• Species: Bacteroides sp900556215
• Lineage: Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Bacteroidaceae; Bacteroides; Bacteroides sp900556215
• CAZyme ID: MGYG000000211_04634
• CAZy Family: GH116
• CAZyme Description: hypothetical protein

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
851	MGYG000000211_12|CGC3	96096.45	5.6754

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000000211	6486320	Isolate	China	Asia

• Gene Location: Start: 187060; End: 189615 Strand: -

Full Sequence      

MKNLLVLGTIVLISLSSCRNERQNEPQISEYNKEYKGEFLNRIAFPIGGMGAGMFCLEGT
GAISNMSVRHQPNIFNEPCMFAAIHVKGYDKGSKVLEGPVPDRKKFGGPESALGGPLTSW
GLPRFEDASFSSRFPFASIRLSDKDIPLNVELKGWSPFIPTDQDNSSLPVGALEYVFTNT
SDQDVEAIFSYNTRNFMAMHGAPRTIRAMKNGFILSQNASDNEKHLKGDFAIYTNTDATK
VNCCWFRGGWWDPLTMAWNAIADGKIVENAPVKEEAPGASLYVPFKVKAGETYSVRLQMA
WYVPDSNMRIGADAVSEDDKSSECPIVEKVETPQYYRPWYSSRFADINEVSEYWLSKYDE
LKQKTELFTNTFYNTTLPAEVIDAVSSNLSILKSPTVMRQHDGRLWNFEGCGDTWGSCHG
SCTHVWNYAQAIPHLFPAMERTLRESEFFISQNKEGHQMFRTNIPIRAVKHDFHAAADGQ
LGGIIKIYRDWRICGDTDWLRMMYPQVKNSLDYCINTWDPRRTGALEEPHHNTYDIEFWG
ADGMCTSFYAAALQSFIQLGKALNENVSEYESLLAKSKDYMETKLYDGEYFIQNIQWENL
KSPDPIAIAEKNWNSDYSEEARAILIKEGPKYQYGKGCLSDGIIGCWMSLVAGLDEPIDK
AKVKSHLNSIYKYNLRKDLSDHANPQRPTYGLGKDGGVLLCTWPKGGKLSLPFVYSDEVW
TGIEYQVASHLIFEGEVEKGLDIVRTVRERYDGKVRNPFNEYECGGWYARALSSYSLLQA
LTGLRYDAVDHTLYIDSRIGNSFKTFLSTNTGFGTVEMQQGKPIINVVYGSLDIQSCVVS
GNKTDFKYQTN

Enzyme Prediction     

No EC number prediction in MGYG000000211_04634.

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GH116	455	777	1e-58	0.7796143250688705

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
pfam04685	DUF608	1.43e-61	403	777	1	362	Glycosyl-hydrolase family 116, catalytic region. This represents a family of archaeal, bacterial and eukaryotic glycosyl hydrolases, that belong to superfamily GH116. The primary catabolic pathway for glucosylceramide is catalysis by the lysosomal enzyme glucocerebrosidase. In higher eukaryotes, glucosylceramide is the precursor of glycosphingolipids, a complex group of ubiquitous membrane lipids. Mutations in the human protein cause motor-neurone defects in hereditary spastic paraplegia. The catalytic nucleophile, identified in UniProtKB:Q97YG8_SULSO, is a glutamine-335, with the likely acid/base at Asp-442 and the aspartates at Asp-406 and Asp-458 residues also playing a role in the catalysis of glucosides and xylosides that are beta-bound to hydrophobic groups. The family is defined as GH116, which presently includes enzymes with beta-glucosidase, EC:3.2.1.21, beta-xylosidase, EC:3.2.1.37, and glucocerebrosidase EC:3.2.1.45 activity.
pfam12215	Glyco_hydr_116N	1.15e-50	44	361	1	309	beta-glucosidase 2, glycosyl-hydrolase family 116 N-term. This domain is found in bacteria, archaea and eukaryotes. This domain is typically between 320 to 354 amino acids in length. This domain is found associated with pfam04685. It is found just after the extreme N-terminus. The N-terminal is thought to be the luminal domain while the C terminal is the cytosolic domain. The catalytic domain of GBA-2 is unknown. The primary catabolic pathway for glucosylceramide is catalysis by the lysosomal enzyme glucocerebrosidase. In higher eukaryotes, glucosylceramide is the precursor of glycosphingolipids, a complex group of ubiquitous membrane lipids. Mutations in the human protein cause motor-neurone defects in hereditary spastic paraplegia. The catalytic nucleophile, identified in UniProtKB:Q97YG8_SULSO, is a glutamine-335 in the downstream family pfam04685.
COG4354	COG4354	4.38e-30	46	785	16	721	Uncharacterized protein, contains GBA2_N and DUF608 domains [Function unknown].

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
QDO68263.1	0.0	1	851	1	851
QUT37400.1	0.0	1	849	1	850
QBJ18460.1	0.0	1	849	1	850
QUT60790.1	0.0	31	843	30	848
QQA29305.1	0.0	31	843	30	848

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
5FJS_A	1.99e-17	46	739	55	732	Bacterialbeta-glucosidase reveals the structural and functional basis of genetic defects in human glucocerebrosidase 2 (GBA2) [Thermoanaerobacterium xylanolyticum LX-11],5FJS_B Bacterial beta-glucosidase reveals the structural and functional basis of genetic defects in human glucocerebrosidase 2 (GBA2) [Thermoanaerobacterium xylanolyticum LX-11],5O0S_A Crystal structure of txGH116 (beta-glucosidase from Thermoanaerobacterium xylolyticum) in complex with unreacted beta Cyclophellitol Cyclosulfate probe ME711 [Thermoanaerobacterium xylanolyticum LX-11]
5BVU_A	2.01e-17	46	739	59	736	Crystalstructure of Thermoanaerobacterium xylolyticum GH116 beta-glucosidase [Thermoanaerobacterium xylanolyticum LX-11],5BX2_A Crystal structure of Thermoanaerobacterium xylanolyticum GH116 beta-glucosidase with 2-deoxy-2-fluoroglucoside [Thermoanaerobacterium xylanolyticum LX-11],5BX3_A Crystal structure of Thermoanaerobacterium xylanolyticum GH116 beta-glucosidase with deoxynojirimycin [Thermoanaerobacterium xylanolyticum LX-11],5BX4_A Crystal structure of Thermoanaerobacterium xylanolyticum GH116 beta-glucosidase with Glucoimidazole [Thermoanaerobacterium xylanolyticum LX-11],5BX5_A Crystal structure of Thermoanaerobacterium xylanolyticum GH116 beta-glucosidase with glucose [Thermoanaerobacterium xylanolyticum LX-11],5NCX_A Crystal structure of Thermoanaerobacterium xylolyticum GH116 beta-glucosidase with an covalent inhibitor [Thermoanaerobacterium xylanolyticum LX-11],5OST_A Beta-glucosidase from Thermoanaerobacterium xylolyticum GH116 in complex with Gluco-1H-imidazole [Thermoanaerobacterium xylanolyticum LX-11]
5NPF_A	2.01e-17	46	739	58	735	Crystalstructure of txGH116 (beta-glucosidase from Thermoanaerobacterium xylolyticum) in complex with beta Cyclophellitol Cyclosulfate probe ME594 [Thermoanaerobacterium xylanolyticum]
7W2V_A	2.10e-17	46	739	102	779	ChainA, Glucosylceramidase [Thermoanaerobacterium xylanolyticum LX-11]
7W2W_A	2.10e-17	46	739	102	779	ChainA, Glucosylceramidase [Thermoanaerobacterium xylanolyticum LX-11],7W2X_A Chain A, Glucosylceramidase [Thermoanaerobacterium xylanolyticum LX-11]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
Q9HCG7	2.96e-07	471	758	612	863	Non-lysosomal glucosylceramidase OS=Homo sapiens OX=9606 GN=GBA2 PE=1 SV=2
Q5M868	1.15e-06	471	758	603	854	Non-lysosomal glucosylceramidase OS=Rattus norvegicus OX=10116 GN=Gba2 PE=2 SV=2
Q69ZF3	1.15e-06	471	758	603	854	Non-lysosomal glucosylceramidase OS=Mus musculus OX=10090 GN=Gba2 PE=1 SV=2
Q7KT91	1.53e-06	499	781	687	940	Non-lysosomal glucosylceramidase OS=Drosophila melanogaster OX=7227 GN=CG33090 PE=1 SV=1

SignalP and Lipop Annotations

This protein is predicted as LIPO

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
0.000000	0.000170	0.999895	0.000000	0.000000	0.000000

TMHMM  Annotations     

There is no transmembrane helices in MGYG000000211_04634.