CAZyme Information: MGYG000000217_02759

Basic Information

• Species: Acetatifactor sp900066565
• Lineage: Bacteria; Firmicutes_A; Clostridia; Lachnospirales; Lachnospiraceae; Acetatifactor; Acetatifactor sp900066565
• CAZyme ID: MGYG000000217_02759
• CAZy Family: CBM48
• CAZyme Description: 1,4-alpha-glucan branching enzyme GlgB

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
778	MGYG000000217_21|CGC2	88972.1	5.3973

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000000217	3653823	Isolate	China	Asia

• Gene Location: Start: 44134; End: 46470 Strand: +

Full Sequence      

MTNKLYKLMNWAKIEEITYAECDNPHELLGPHKQGSKMLVQAFFPGAEKVTIHWKKTGQV
GEAFLADVPMELADEEGYFAALVNAGKMAPYEYVVEYGKTEEREAHRMICGDAYRHVPQI
TKEDMDRFAAGIHYTIYEKLGAHPMELDGDNGTYFAVWAPNAMRVSVVGDFNEWDGRIHQ
MRRLGDSGIFELFVPGAEVGDCYKYELKIKGGLTYLKADPYGNAAQKRPETASVITDLRD
FSWDDSEFLKKRESYQCGNAPVSVYEMYLGSFVTPDEGQDYANYREIAPKVIDYVKKMGY
THVELMPVMEHPLDASWGYQVIGYYAPTARYGTPEDFMFFVNELHKAGIGVILDWVPAHF
PRDSYGLAAFDGTCLYEHQDPRQGSHPHWGTLIYNYGRPQVSNYLIANALFWVEKYHADG
IRMDAVASMLYLDYGRQDGEWIPNMYGGNENLEAIELIKHLNSILKKRDPGVLSIAEEST
AFPMITGSLEEGGLGFDLKWNMGFMNDYLDYIKYDPYFRSHHHGELTFSMIYAYSEKFML
VFSHDEVVHGKGTLLGKMPGDRAQKLANLRLTYGYMMTHPGKKLLFMGQDLAEEKEWNEM
RQVEWALQEQKENAGVQRLVKDLNALYRENKALYECDDQAKGFQWMNEISANECYVSFVR
KGEAAEEMLLVVANFSGVPREITTGVPYEGKYKEILNTDAVCYGGTGVVNDRMKRAEDLE
WDDKKQSVTVKLAPLSLSILQFIPYTEAELEKVIEKRIRKNTPIRKTTNKTAKKKQEK

Enzyme Prediction     

EC	2.4.1.18

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GH13	293	593	1.7e-154	0.9966777408637874
CBM48	139	225	7.3e-19	0.881578947368421

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
PRK12313	PRK12313	0.0	115	742	2	627	1,4-alpha-glucan branching protein GlgB.
PRK14705	PRK14705	0.0	14	744	508	1222	glycogen branching enzyme; Provisional
COG0296	GlgB	0.0	128	740	14	626	1,4-alpha-glucan branching enzyme [Carbohydrate transport and metabolism].
PRK14706	PRK14706	0.0	140	757	27	636	glycogen branching enzyme; Provisional
PRK05402	PRK05402	0.0	14	741	6	722	1,4-alpha-glucan branching protein GlgB.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
QNM03882.1	0.0	1	778	1	778
QNM09059.1	0.0	1	769	1	755
AWY97839.1	0.0	1	773	1	761
QRV19504.1	0.0	1	750	1	750
ADL06402.1	0.0	1	750	1	750

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
5GQZ_A	8.13e-237	13	744	29	775	Crystalstructure of branching enzyme Y500A mutant from Cyanothece sp. ATCC 51142 [Crocosphaera subtropica ATCC 51142]
5GQU_A	1.15e-236	13	744	29	775	Crystalstructure of branching enzyme from Cyanothece sp. ATCC 51142 [Crocosphaera subtropica ATCC 51142],5GQV_A Crystal structure of branching enzyme from Cyanothece sp. ATCC 51142 in complex with maltohexaose [Crocosphaera subtropica ATCC 51142],5GQY_A Crystal structure of branching enzyme from Cyanothece sp. ATCC 51142 in complex with maltoheptaose [Crocosphaera subtropica ATCC 51142]
5GR5_A	1.15e-236	13	744	29	775	Crystalstructure of branching enzyme W610A mutant from Cyanothece sp. ATCC 51142 [Crocosphaera subtropica ATCC 51142]
5GQW_A	1.63e-236	13	744	29	775	Crystalstructure of branching enzyme W610N mutant from Cyanothece sp. ATCC 51142 [Crocosphaera subtropica ATCC 51142],5GQX_A Crystal structure of branching enzyme W610N mutant from Cyanothece sp. ATCC 51142 in complex with maltoheptaose [Crocosphaera subtropica ATCC 51142]
5GR1_A	2.31e-236	13	744	29	775	Crystalstructure of branching enzyme Y500A/D501A mutant from Cyanothece sp. ATCC 51142 in complex with maltoheptaose [Crocosphaera subtropica ATCC 51142],5GR6_A Crystal structure of branching enzyme Y500A/D501A double mutant from Cyanothece sp. ATCC 51142 [Crocosphaera subtropica ATCC 51142]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
Q1AZ86	1.19e-254	14	741	7	718	1,4-alpha-glucan branching enzyme GlgB OS=Rubrobacter xylanophilus (strain DSM 9941 / NBRC 16129 / PRD-1) OX=266117 GN=glgB PE=3 SV=1
Q8DLB8	2.70e-254	13	761	7	763	1,4-alpha-glucan branching enzyme GlgB OS=Thermosynechococcus vestitus (strain IAM M-273 / NIES-2133 / BP-1) OX=197221 GN=glgB PE=3 SV=1
Q608L5	8.73e-249	15	740	15	723	1,4-alpha-glucan branching enzyme GlgB OS=Methylococcus capsulatus (strain ATCC 33009 / NCIMB 11132 / Bath) OX=243233 GN=glgB PE=3 SV=1
Q2JT08	1.95e-247	8	749	4	760	1,4-alpha-glucan branching enzyme GlgB OS=Synechococcus sp. (strain JA-3-3Ab) OX=321327 GN=glgB PE=3 SV=1
B3PGN4	1.34e-244	17	741	17	735	1,4-alpha-glucan branching enzyme GlgB OS=Cellvibrio japonicus (strain Ueda107) OX=498211 GN=glgB PE=3 SV=1

SignalP and Lipop Annotations

This protein is predicted as OTHER

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
1.000045	0.000000	0.000000	0.000000	0.000000	0.000000

TMHMM  Annotations     

There is no transmembrane helices in MGYG000000217_02759.