CAZyme Information: MGYG000000224_01121

Basic Information

• Species: Bacteroides sp003545565
• Lineage: Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Bacteroidaceae; Bacteroides; Bacteroides sp003545565
• CAZyme ID: MGYG000000224_01121
• CAZy Family: GH127
• CAZyme Description: Non-reducing end beta-L-arabinofuranosidase

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
619	MGYG000000224_3|CGC5	70546.43	7.2999

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000000224	3747034	Isolate	China	Asia

• Gene Location: Start: 334957; End: 336816 Strand: +

Full Sequence      

MKQFTKTILVCGMTLICYTGGDKTLFAQTKMINQVDFSQVRIQDDFWSPRLKKHVTNTLP
VCIDQIENKTGRIRNFENAAKNSGKHSGIFYDDSDVYKALEGIAYSLINNPNPELEKKAD
EWINKMAAAQLPDGYINTFYTLTGLDKRWTNMDKHEMYCAGHMIEAAVAYYKATGKRTLL
NVSTHMVDHMMTLFGPNKRHWVPGHEEIELALVKLAQTTGESKYVDFANWLLEQRGHGYG
TKGDKSNWDKSYYQDEVPVSEMESIGGHAVRCMYLYCGMADVAAIKHNPQYIDALNRLWT
DVVERNMYITGGIGSSRHNEGFTEDYDLPNLEAYCETCASVGMVLWNHRMNQFTGDSKYI
DVLERSMYNGALAGISLNGDRFFYVNPLESKGDHHRLPWYGCACCPSQLSRFLPSIGNYI
YGISDNAIWVNLYIGNVAEVNVDGVQVAMKEETKYPWDGKIKFTINADEKINKELRLRIP
GWCKKYNLFINGKKVKKQVVDKGYVVIAEWNSGDNIELDFDMPVEVVQSDIRVKQNIGKR
AIQRGPLVYCIEDAQNKGTIEGIHISPKTSFKTNFNVNLLNGVQQITAKDGKKTFTLIPY
YAWDNMEAGNMKVWIDYKK

Enzyme Prediction     

No EC number prediction in MGYG000000224_01121.

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GH127	40	550	6.2e-207	0.9980916030534351

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
COG3533	COG3533	3.71e-178	32	619	14	589	Uncharacterized conserved protein, DUF1680 family [Function unknown].
pfam07944	Glyco_hydro_127	2.28e-177	39	550	1	503	Beta-L-arabinofuranosidase, GH127. One member of this family, from Bidobacterium longicum, UniProtKB:E8MGH8, has been characterized as an unusual beta-L-arabinofuranosidase enzyme, EC:3.2.1.185. It rleases l-arabinose from the l-arabinofuranose (Araf)-beta1,2-Araf disaccharide and also transglycosylates 1-alkanols with retention of the anomeric configuration. Terminal beta-l-arabinofuranosyl residues have been found in arabinogalactan proteins from a mumber of different plantt species. beta-l-Arabinofuranosyl linkages with 1-4 arabinofuranosides are also found in the sugar chains of extensin and solanaceous lectins, hydroxyproline (Hyp)2-rich glycoproteins that are widely observed in plant cell wall fractions. The critical residue for catalytic activity is Glu-338, in a ET/SCAS sequence context.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
QUT44636.1	0.0	1	619	1	619
ABR45039.1	0.0	1	619	1	617
AST55952.1	0.0	1	619	1	617
QUT97168.1	0.0	1	619	1	617
QKH99528.1	0.0	1	619	1	617

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
6EX6_A	4.51e-164	32	615	7	623	TheGH127, Beta-arabinofuranosidase, BT3674 [Bacteroides thetaiotaomicron VPI-5482],6EX6_B The GH127, Beta-arabinofuranosidase, BT3674 [Bacteroides thetaiotaomicron VPI-5482]
4QJY_A	3.70e-157	38	618	14	648	Crystalstructure of native Ara127N, a GH127 beta-L-arabinofuranosidase from Geobacillus Stearothermophilus T6 [Geobacillus stearothermophilus],4QJY_B Crystal structure of native Ara127N, a GH127 beta-L-arabinofuranosidase from Geobacillus Stearothermophilus T6 [Geobacillus stearothermophilus]
4QK0_A	1.88e-151	38	618	14	648	Crystalstructure of Ara127N-Se, a GH127 beta-L-arabinofuranosidase from Geobacillus Stearothermophilus T6 [Geobacillus stearothermophilus],4QK0_B Crystal structure of Ara127N-Se, a GH127 beta-L-arabinofuranosidase from Geobacillus Stearothermophilus T6 [Geobacillus stearothermophilus],4QK0_C Crystal structure of Ara127N-Se, a GH127 beta-L-arabinofuranosidase from Geobacillus Stearothermophilus T6 [Geobacillus stearothermophilus],4QK0_D Crystal structure of Ara127N-Se, a GH127 beta-L-arabinofuranosidase from Geobacillus Stearothermophilus T6 [Geobacillus stearothermophilus]
3WRE_A	2.20e-116	40	613	3	654	Thecrystal structure of native HypBA1 from Bifidobacterium longum JCM 1217 [Bifidobacterium longum subsp. longum JCM 1217],3WRG_A The complex structure of HypBA1 with L-arabinose [Bifidobacterium longum subsp. longum JCM 1217]
3WKW_A	2.97e-116	40	613	3	654	Crystalstructure of GH127 beta-L-arabinofuranosidase HypBA1 from Bifidobacterium longum ligand free form [Bifidobacterium longum subsp. longum JCM 1217],3WKX_A Crystal structure of GH127 beta-L-arabinofuranosidase HypBA1 from Bifidobacterium longum arabinose complex form [Bifidobacterium longum subsp. longum JCM 1217],7BZL_A Chain A, Non-reducing end beta-L-arabinofuranosidase [Bifidobacterium longum subsp. longum JCM 1217],7DIF_A Chain A, Non-reducing end beta-L-arabinofuranosidase [Bifidobacterium longum subsp. longum JCM 1217],7EXV_A Chain A, Non-reducing end beta-L-arabinofuranosidase [Bifidobacterium longum subsp. longum JCM 1217],7EXW_A Chain A, Non-reducing end beta-L-arabinofuranosidase [Bifidobacterium longum subsp. longum JCM 1217]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
E8MGH8	1.21e-115	40	613	3	654	Non-reducing end beta-L-arabinofuranosidase OS=Bifidobacterium longum subsp. longum (strain ATCC 15707 / DSM 20219 / JCM 1217 / NCTC 11818 / E194b) OX=565042 GN=hypBA1 PE=1 SV=1

SignalP and Lipop Annotations

This protein is predicted as SP

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
0.003551	0.846821	0.148788	0.000346	0.000250	0.000230

TMHMM  Annotations     

There is no transmembrane helices in MGYG000000224_01121.