CAZyme Information: MGYG000000224_01540

Basic Information

• Species: Bacteroides sp003545565
• Lineage: Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Bacteroidaceae; Bacteroides; Bacteroides sp003545565
• CAZyme ID: MGYG000000224_01540
• CAZy Family: GH115
• CAZyme Description: hypothetical protein

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
1003		113555.51	6.9127

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000000224	3747034	Isolate	China	Asia

• Gene Location: Start: 28109; End: 31120 Strand: +

Full Sequence      

MKRKLFFEGIFFLFVCAASLNAQQTMNVIPPIIYDCATDTKDFPVVDTQQRSAIIRYDAD
DYKGVIRAISDLQKDIERVTDVKINTSANGPLGKYEIMIGTLGKSKVIDELVTSGKIKPR
NLKGKWESFIITTVDNPKPGIRQGLIIAGSDKRGTIYGIYELSQQLGVSPWYWWADAPIQ
KRQSAYVKSGYYESGEPKVKYRGIFINDEWPSFGNWATEKFGGVNSKMYVHLFELLLRLK
ANFFWPAMWASAFNEDDPMNPVLADEYGIVMGTSHHEPMMRAHKEYTRRKGEVGPWDYKT
NKERLDNFFREGMERNKEFENVVTIGMRGDGDVAMSEEGDEANMQVLKKVIEGQRQTLKE
VYGKEPSEIPQLWAIFTEVQRYYDAGFTVPDDVMLLFCDNNWGYIRRTGPDKEKKRKGGM
GLYYHIDMNGGPWNDRWVTTTTVPKLREQLHLAYQSSIDDLWVINVGDIKPKELPIDFIM
RYAWNPEAISAEKTGDYMIEWAQSIFGTEYAKDIADIVSKYTKYNLLRKAEVQLPDVFSV
VNYHEADRILAAWKELTAKAEELEHKLSPEAKDAYYQLVLYPVKASAGVAEIYLSVGKNR
LYAKQGRISANDYAARAKELFNLDWYLSNRYNNETADGKWKHMMSDKHIGYTKWYMPENN
ELPELSIINPLGIPTPGIAVEGSEAAWPLMTEHLSLPVFDALNNQTYYVEVFNRGVGEFE
YTVLTSQPWIKVDKTKGKVAKENRMNISIDWSKAPQGTTEGVVEIVCGKHKAEVKVPIVN
GTVPDSKEAFFGTLSDSEFSIPAYAYNANIPGRQAKWSFIPDLGREKGNMGIVPATASST
LAHKSAARLEYKVYLPSAGKTTIALGILPTQDVNPARGLRIAIGIDGKKPVVLDARKGFV
DTFGEYNPKNLALSKVLKPLPERSTLALSGKGKNLRNEVFDNMRWLDVELEVDKPGFHTL
KVYMVDPEVVLEKIVVNPDNNRPSYFGAPSKQHNAATMESTNN

Enzyme Prediction     

EC	3.2.1.131

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GH115	42	974	1.1e-254	0.9956958393113343

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
pfam15979	Glyco_hydro_115	0.0	195	532	1	334	Glycosyl hydrolase family 115. Glyco_hydro_115 is a family of glycoside hydrolases likely to have the activity of xylan a-1,2-glucuronidase, EC:3.2.1.131, or a-(4-O-methyl)-glucuronidase EC:3.2.1.-.
pfam17829	GH115_C	2.48e-35	800	989	10	171	Gylcosyl hydrolase family 115 C-terminal domain. This domain is found at the C-terminus of glycosyl hydrolase family 115 proteins. This domain has a beta-sandwich fold.
pfam03648	Glyco_hydro_67N	2.48e-04	68	162	36	120	Glycosyl hydrolase family 67 N-terminus. Alpha-glucuronidases, components of an ensemble of enzymes central to the recycling of photosynthetic biomass, remove the alpha-1,2 linked 4-O-methyl glucuronic acid from xylans. This family represents the N-terminal region of alpha-glucuronidase. The N-terminal domain forms a two-layer sandwich, each layer being formed by a beta sheet of five strands. A further two helices form part of the interface with the central, catalytic, module (pfam07488).

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
EEC55419.1	0.0	1	999	1	999
QRQ50360.1	0.0	26	999	1	974
QUT44306.1	0.0	179	999	1	821
QDW23484.1	0.0	1	993	1	987
QUT25621.1	0.0	4	990	6	1006

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
4ZMH_A	5.61e-247	49	987	19	929	Crystalstructure of a five-domain GH115 alpha-Glucuronidase from the Marine Bacterium Saccharophagus degradans 2-40T [Saccharophagus degradans 2-40],4ZMH_B Crystal structure of a five-domain GH115 alpha-Glucuronidase from the Marine Bacterium Saccharophagus degradans 2-40T [Saccharophagus degradans 2-40]
4C90_A	2.13e-173	55	682	55	674	Evidencethat GH115 alpha-glucuronidase activity is dependent on conformational flexibility [Bacteroides ovatus],4C90_B Evidence that GH115 alpha-glucuronidase activity is dependent on conformational flexibility [Bacteroides ovatus],4C91_A Evidence that GH115 alpha-glucuronidase activity is dependent on conformational flexibility [Bacteroides ovatus],4C91_B Evidence that GH115 alpha-glucuronidase activity is dependent on conformational flexibility [Bacteroides ovatus]
7PUG_A	3.34e-171	55	654	28	635	ChainA, xylan alpha-1,2-glucuronidase [uncultured bacterium]
7PXQ_A	1.51e-168	55	654	27	634	ChainA, xylan alpha-1,2-glucuronidase [uncultured bacterium]
6NPS_A	1.87e-158	48	987	14	959	Crystalstructure of GH115 enzyme AxyAgu115A from Amphibacillus xylanus [Amphibacillus xylanus NBRC 15112],6NPS_B Crystal structure of GH115 enzyme AxyAgu115A from Amphibacillus xylanus [Amphibacillus xylanus NBRC 15112]

Swiss-Prot Hits     

has no Swissprot hit.

SignalP and Lipop Annotations

This protein is predicted as SP

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
0.000771	0.753929	0.244404	0.000359	0.000278	0.000241

TMHMM  Annotations     

There is no transmembrane helices in MGYG000000224_01540.