CAZyme Information: MGYG000000240_00902

Basic Information

• Species: Amedibacterium intestinale
• Lineage: Bacteria; Firmicutes; Bacilli; Erysipelotrichales; Erysipelotrichaceae; Amedibacterium; Amedibacterium intestinale
• CAZyme ID: MGYG000000240_00902
• CAZy Family: GH101
• CAZyme Description: hypothetical protein

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
2192		245769.78	4.6642

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000000240	2625986	Isolate	China	Asia

• Gene Location: Start: 21859; End: 28437 Strand: +

Full Sequence      

MKKQKFISGMAAVLTVCMNVSALPVSAISSQVFAIDDLVEFTDKTDRGKWDKTSGNGSIS
FQDGQGDNGFMTVKSNGDTIFADTSTTKRADGFVEMDMKMTEAPTGARMAIIFRYNSPTD
WEGIGIDHGDWTWLKGSDKWGSVSSTKKIFTSVGEKHHLRVEYRGSNLKVFVDGEQVINQ
DIKELNGAKEGNIGIRLWGDIQENFDGHCAFQVDNVTTGDLQVEAGISPDNVTIPYEEAG
KEDVVFTMTEVSPNLKMIKNGDVSLIQGKDYTIDGTNVILSKTYVESLKETAVTDLTFVF
EDEQEKTCHLFIEKQEEVVSYIRNFKEGKDGFEKVLGSGSLQVNKDSVTIQGDGVFVDKN
SKILKNQEVEFTYDPINNNCNYGVILRYTSPSEYIYVGPSGQNAQHYTKWGIYGPNGLIQ
EIEDSGFILEGREVPYNVKVRLVDNIITIYVDKEEIWNGTIDGITMKPGKTGFRTTNNAG
MTIQGFTQETAVKYKKAANVTSKQIQSNEMSVKMDTAFPRVEEYTLTTGEKVKGQKIALH
QVEINNKLYTPKVTSEINGNKAMYHMNIDELKLSFDVIFTVEKNVLSMDVENIKESDTKL
YTLNFPRHSLISMSSKDPSAQLRVNNYKEEKRINLKDAKPFEHYNETTLTVLSNDNVAAA
ISGESYKNRHEMAYQTFALGDHTSTGIWVNEYTYRGLDGELYRKHYSDEQKNEPWARVAI
TTDCNKDSKVDYQDGAIVLRDDCMERKTGADTVTDSWNMIAMNVGSEAQYPFLRILDNAK
KMSLATDNFSQNIIIKGYQSEGHDASHPDFANYNKRAGGLEDFKTLLEESQNYNTKVGVH
INHTDVYPEAPQYEKIKTELGAWSWYDSAKQIVRENDDLDKTSQGLDGRLEQLFDKDTEN
MLDTVYVDVFFGTRWPMYKLVDNINSRDMALGTEYVDEMVSHSVFAHHIGSNFGGAGNLV
RIVDNSQADIFANHQLFRGAASRANEDIGIDGWQGAKDMNKAIQSFYEKILPNKFLAQYP
IMQYSSDEKAVLGNKNEVVTKMENGKNIITLNEKAVAKGNQIFIPWENEDKEKEGKIYHW
NPEGGTTTWELPDSWSNVTTVKLYELSDEGKKEVTSEVIQKDGNRVTINAKAKTGYVLYK
DKVAQKDMKTADTMEWSSGSPVKDMGFDSHNFDEWKPSSSAENTDHITIENNEIGNSHLY
IKGENDGKVTQTLTGLIPGQTYAASVWCITDDGRKASIEVQNGDEVAVNYMNRSNVTYGV
HHNDKYKTKAQRMQVRFTALSDSATLTLSAAKGKDAQSIVDFDDVRVVKVDPSTNPDPKK
YTYWEDFENTDQGWGAFISTESDQSHLSQKNPVNPEVTDDVIDGNYSLKVRAGDYMRTIP
STVRLEPETEYKIGISYKSPAANAFIFAVKSDKAKEANDEAHAVIASIDAIDKEGNLELK
FTTGNYDDYYIDITKKAGSEYYLDNFYVQAARPINRETLGQLIQEAKQLEERAYTPESYA
KVVEAINKASKVLDDAQASKEEIRDAYKGLEAAIEQLVPYALTEEKASLMEIVTTMKSLH
ATDYKQDDKWMKLQTLIKEAEALYKNDKATSVQVSDMIRDLNDAKDALNPVVDRSALKEI
MKKAERVDRNAVVDGAELQTFLSAIENAKAVDIKPGVTEEEIASATKKLQDAYNMILLKE
DMKNQMVNEALKKADVKEEYYLEEDWKAITEAKTALQEMSTQTNVKVADYYEVLDKLETA
LNNKLNRPVISSSIEIDSSAFDIKSNNEQPQTGNEGPIALAFDQNPNTLWHSKYEPKVPV
TAQNPAQITIDMKNVYTVNQFSYLQRLTGDNGKIKKFNLYVKNNESDDWTKIVADGTFKN
VNEVQKVKFEPVQAQYVMFEVTEGIGNFASAAELAVYEQASDFSNLQKAMSEFEKKDPKQ
YTTASYTAVKKVYDEAVAMLDNPLTEQKKIDDITGKLKQAITSLELLATGTDIQMLENAI
EEAKKIDLTEYKDTKAFEAALKEALDVQKDMQDGKEVTQKEVTSAALALVEEMNNLVPVD
KPSVDYSKLQEEIDSAIHDSQASKYPVQLWDAYQNALNHAKTVLSKADATQEEVDNALKQ
LQDTRKALEDSLKDDGSIPSTPIEPGKPIVVPEPPKNPEEGQNETDTSEKTDKEENESSI
KTGVRSNTAILGITTIAALGAMVELIRKKRKN

Enzyme Prediction     

No EC number prediction in MGYG000000240_00902.

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GH101	507	1038	3.6e-99	0.8104667609618105
CBM32	1764	1894	1.1e-20	0.9435483870967742

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
cd14244	GH_101_like	1.83e-84	746	1026	1	298	Endo-a-N-acetylgalactosaminidase and related glcyosyl hydrolases. This family contains the enzymatically active domain of cell surface proteins that specifically cleave Gal-beta-1,3-GalNAc-alpha-Ser/Thr (T-antigen, galacto-N-biose), the core 1 type O-linked glycan common to mucin glycoproteins (EC:3.2.1.97). It has been classified as glycosyl hydrolase family 101 in the Cazy resource. Virulence of pathogenic organisms such as the Gram-positive Streptococcus pneumoniae and other commensal human bacteria is largely determined by their ability to degrade host glycoproteins and to metabolize the resultant carbohydrates.
pfam12905	Glyco_hydro_101	6.24e-66	733	1003	1	273	Endo-alpha-N-acetylgalactosaminidase. Virulence of pathogenic organisms such as the Gram-positive Streptococcus pneumoniae is largely determined by the ability to degrade host glycoproteins and to metabolize the resultant carbohydrates. This family is the enzymatic region, EC:3.2.1.97, of the cell surface proteins that specifically cleave Gal-beta-1,3-GalNAc-alpha-Ser/Thr (T-antigen, galacto-N-biose), the core 1 type O-linked glycan common to mucin glycoproteins. This reaction is exemplified by the S. pneumoniae protein Endo-alpha-N-acetylgalactosaminidase, where Asp764 is the catalytic nucleophile-base and Glu796 the catalytic proton donor.
pfam18080	Gal_mutarotas_3	1.21e-49	505	732	1	243	Galactose mutarotase-like fold domain. This domain is found in endo-alpha-N-acetylgalactosaminidase present in Streptococcus pneumoniae. Endo-alpha-N-acetylgalactosaminidase is a cell surface-anchored glycoside hydrolase involved in the breakdown of mucin type O-linked glycans. The domain, known as domain 2, exhibits strong structural similarlity to the galactose mutarotase-like fold but lacks the active site residues. Domains, found in a number of glycoside hydrolases, structurally similar to domain 2 confer stability to the multidomain architectures.
pfam17451	Glyco_hyd_101C	7.26e-26	1011	1111	3	110	Glycosyl hydrolase 101 beta sandwich domain. Virulence of pathogenic organisms such as the Gram-positive Streptococcus pneumoniae is largely determined by the ability to degrade host glycoproteins and to metabolize the resultant carbohydrates. This family is the enzymatic region, EC:3.2.1.97, of the cell surface proteins that specifically cleave Gal-beta-1,3-GalNAc-alpha-Ser/Thr (T-antigen, galacto-N-biose), the core 1 type O-linked glycan common to mucin glycoproteins. This reaction is exemplified by a S. pneumoniae protein, where Asp764 is the catalytic nucleophile-base and Glu796 the catalytic proton donor. This domain represents C-terminal the beta sandwich domain.
pfam00754	F5_F8_type_C	9.61e-15	1771	1894	7	127	F5/8 type C domain. This domain is also known as the discoidin (DS) domain family.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
BBK62189.1	0.0	1	2192	1	2192
BBK22105.1	0.0	1	2192	1	2192
ATD58436.1	0.0	38	1746	229	1897
ATD54117.1	0.0	38	1746	229	1897
QBJ76357.1	0.0	38	1746	229	1897

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
6QEP_A	9.21e-91	500	1409	7	1040	EngBFDARPin Fusion 4b H14 [Bifidobacterium longum]
2ZXQ_A	1.12e-90	500	1409	22	1055	Crystalstructure of endo-alpha-N-acetylgalactosaminidase from Bifidobacterium longum (EngBF) [Bifidobacterium longum]
6QFK_A	3.95e-90	500	1409	7	1040	EngBFDARPin Fusion 4b G10 [Bifidobacterium longum]
6QEV_B	3.95e-90	500	1409	7	1040	EngBFDARPin Fusion 4b B6 [Bifidobacterium longum]
6SH9_B	3.95e-90	500	1409	7	1040	EngBFDARPin Fusion 4b D12 [Bifidobacterium longum subsp. longum JCM 1217]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
A9WNA0	5.15e-89	505	1425	52	991	Putative endo-alpha-N-acetylgalactosaminidase OS=Renibacterium salmoninarum (strain ATCC 33209 / DSM 20767 / JCM 11484 / NBRC 15589 / NCIMB 2235) OX=288705 GN=RSal33209_1326 PE=3 SV=2
Q8DR60	2.51e-87	498	1409	327	1332	Endo-alpha-N-acetylgalactosaminidase OS=Streptococcus pneumoniae (strain ATCC BAA-255 / R6) OX=171101 GN=spr0328 PE=1 SV=1
Q2MGH6	4.35e-87	498	1409	327	1332	Endo-alpha-N-acetylgalactosaminidase OS=Streptococcus pneumoniae serotype 4 (strain ATCC BAA-334 / TIGR4) OX=170187 GN=SP_0368 PE=1 SV=1
P0DTR4	4.92e-11	1775	1897	527	644	A type blood N-acetyl-alpha-D-galactosamine deacetylase OS=Flavonifractor plautii OX=292800 PE=1 SV=1

SignalP and Lipop Annotations

This protein is predicted as SP

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
0.000302	0.998689	0.000462	0.000206	0.000167	0.000155

TMHMM  Annotations     

There is no transmembrane helices in MGYG000000240_00902.