CAZyme Information: MGYG000000243_00170

Basic Information

• Species: Phocaeicola massiliensis
• Lineage: Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Bacteroidaceae; Phocaeicola; Phocaeicola massiliensis
• CAZyme ID: MGYG000000243_00170
• CAZy Family: GH2
• CAZyme Description: Evolved beta-galactosidase subunit alpha

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
1340	MGYG000000243_1|CGC4	151123.93	6.5803

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000000243	3947824	Isolate	China	Asia

• Gene Location: Start: 213443; End: 217465 Strand: -

Full Sequence      

MQKLAFSLLLCCFTLQSYAKVEPLKGFHYASEQAPKGNEWESPENLALNKEQPHAYFFSF
QDKASARKVLPENSAYWQSLNGDWKFNWVPNPEERPKDFYKTEFDVSGWDNIPVPSSWNI
YGIQKDGTQKYGTPIYVNQPVIFQHKVAVDDWRGGVMRTPPSNWTTFKARNEVGSYRREF
TIPEDWDGKEIFINFDGVDSFFYLWINGQYVGFSKNSRNTASFNITKYLVKGTNVVAVEV
YRSSDGSFLEAQDMFRLPGIYRTVALQAVPQLHVRDLVVTPDLDATYTDGELKINADIRN
LGKKAAKGYSMVYSLYANQLYSDDNTEVNNAGISTPVAVVEAGQEVTAETILKVQKPNKW
SAEKPYLYTLVGELKDKKGRTLETVSTHIGFRKVEIKDTPASEDEFGLAGRYYYVNGKTV
KLKGVNRHETNPGVGHAITRKMMENEIMMMKRANINHVRNSHYPDDPYWYYLCNKYGIYL
EDEANIESHEYYYGKASLSHPIEWKNAHVARVMEMVHANINNPSIVIWSLGNEAGPGQNF
VAAYDALKVADKSRPVQYERNNDIVDMGSNQYPSISWVRGAVKGDYDIKYPFHISEYAHS
MGNACGNLIDYWDAIESTNFFCGGAIWDWVDQAMYNYTKDGKRYLAYGGDFGDTPNDGQF
VMNGIVFADLEPKPQYFETKKVYQHIGVKAIDEKQGRFEIFNKYYFKDLSEYEIRWALYE
NGKEAENGVLNTENIAPRTRLQVTIPYRYNNLKANAEYFVKVQFVLRNNMPWAEKGYVMA
EEQLPVKTATGIAPINEVTDGNGKPVMAKQSDPRFSTIKGENFEAKFDNETGTIYSLNYG
GETIIADGNGPKLDAMRAFTNNDNWFYANWFECGLHNLKHQVTASKVINRKDGAIVLFYT
VESQAPNGAKILGGTSSGKNSIKELTEKPFGEDDFKFTTNQVWTVYRDGSIELQASITSN
RPSLVLPRLGYVMKVPQRYENYTYYGRGPIGNYPDRKVGQFIEIHKSTVADQFVNFPKPQ
DMGNHEDVRWCALTDKAGKGAIFIATNRLSTSALQYSALDMILAGHPYQLPKAGDTYLHL
DLAVTGLGGNSCGQGGPLMHDRVFAGQNNIGFIIRPAAQDLSAAAQVAPAGDIPLTITRG
RTGMVELSSIDKDAAILYTINKGKKAKQYTEPISMRDGGTVTAWFANNPSIKTTTTFKKL
ETIQTEVIYASSEEVGDGDAKNLVDGDPATYWHSMYSVTVAKYPHWVDLDAGEVKTIKGF
TYMPRQDSYNGNVKEYTIHVSMDGKNWGEPVQKGKFAGNRDEKKVIFSQPVKGRYIRFTA
LSEQYGQDYASGAELTILAE

Enzyme Prediction     

No EC number prediction in MGYG000000243_00170.

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GH2	69	1002	5.9e-215	0.9707446808510638
CBM32	1210	1335	6.3e-27	0.9112903225806451

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
PRK10340	ebgA	0.0	38	1128	2	1011	cryptic beta-D-galactosidase subunit alpha; Reviewed
PRK09525	lacZ	0.0	38	997	15	914	beta-galactosidase.
COG3250	LacZ	1.33e-129	70	837	5	684	Beta-galactosidase/beta-glucuronidase [Carbohydrate transport and metabolism].
smart01038	Bgal_small_N	1.17e-76	819	1114	1	272	Beta galactosidase small chain. This domain comprises the small chain of dimeric beta-galactosidases EC:3.2.1.23. This domain is also found in single chain beta-galactosidase.
pfam02836	Glyco_hydro_2_C	3.27e-76	413	686	8	300	Glycosyl hydrolases family 2, TIM barrel domain. This family contains beta-galactosidase, beta-mannosidase and beta-glucuronidase activities.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
QCQ47063.1	0.0	1	1340	1	1341
QLK84481.1	0.0	1	1340	1	1341
QCQ49582.1	0.0	1	1340	1	1341
QTO25654.1	0.0	1	1340	1	1341
QCQ38420.1	0.0	1	1340	1	1341

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
3DEC_A	1.28e-189	39	1095	6	976	ChainA, Beta-galactosidase [Bacteroides thetaiotaomicron VPI-5482]
6S6Z_A	7.04e-188	39	1090	3	955	Structureof beta-Galactosidase from Thermotoga maritima [Thermotoga maritima MSB8],6S6Z_B Structure of beta-Galactosidase from Thermotoga maritima [Thermotoga maritima MSB8],6S6Z_C Structure of beta-Galactosidase from Thermotoga maritima [Thermotoga maritima MSB8],6S6Z_D Structure of beta-Galactosidase from Thermotoga maritima [Thermotoga maritima MSB8],6S6Z_E Structure of beta-Galactosidase from Thermotoga maritima [Thermotoga maritima MSB8],6S6Z_F Structure of beta-Galactosidase from Thermotoga maritima [Thermotoga maritima MSB8],6S6Z_G Structure of beta-Galactosidase from Thermotoga maritima [Thermotoga maritima MSB8],6S6Z_H Structure of beta-Galactosidase from Thermotoga maritima [Thermotoga maritima MSB8]
6SD0_A	7.24e-188	39	1090	4	956	Structureof beta-galactosidase from Thermotoga maritima. [Thermotoga maritima MSB8],6SD0_B Structure of beta-galactosidase from Thermotoga maritima. [Thermotoga maritima MSB8],6SD0_C Structure of beta-galactosidase from Thermotoga maritima. [Thermotoga maritima MSB8],6SD0_D Structure of beta-galactosidase from Thermotoga maritima. [Thermotoga maritima MSB8]
3BGA_A	6.37e-184	39	1095	9	980	Crystalstructure of beta-galactosidase from Bacteroides thetaiotaomicron VPI-5482 [Bacteroides thetaiotaomicron VPI-5482],3BGA_B Crystal structure of beta-galactosidase from Bacteroides thetaiotaomicron VPI-5482 [Bacteroides thetaiotaomicron VPI-5482]
3MUY_1	8.95e-120	39	1090	15	996	Chain1, Beta-D-galactosidase [Escherichia coli K-12],3MUY_2 Chain 2, Beta-D-galactosidase [Escherichia coli K-12],3MUY_3 Chain 3, Beta-D-galactosidase [Escherichia coli K-12],3MUY_4 Chain 4, Beta-D-galactosidase [Escherichia coli K-12]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
O52847	4.46e-200	25	1094	6	1010	Beta-galactosidase OS=Priestia megaterium (strain DSM 319 / IMG 1521) OX=592022 GN=bgaM PE=3 SV=1
Q56307	3.96e-187	39	1090	4	956	Beta-galactosidase OS=Thermotoga maritima (strain ATCC 43589 / DSM 3109 / JCM 10099 / NBRC 100826 / MSB8) OX=243274 GN=lacZ PE=1 SV=2
P06864	1.12e-162	38	1128	2	1011	Evolved beta-galactosidase subunit alpha OS=Escherichia coli (strain K12) OX=83333 GN=ebgA PE=1 SV=4
A1SWB8	5.65e-157	36	1101	9	1017	Beta-galactosidase OS=Psychromonas ingrahamii (strain 37) OX=357804 GN=lacZ PE=3 SV=1
Q9K9C6	9.46e-155	46	1113	16	1008	Beta-galactosidase OS=Alkalihalobacillus halodurans (strain ATCC BAA-125 / DSM 18197 / FERM 7344 / JCM 9153 / C-125) OX=272558 GN=lacZ PE=3 SV=1

SignalP and Lipop Annotations

This protein is predicted as SP

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
0.000749	0.951002	0.047388	0.000283	0.000284	0.000263

TMHMM  Annotations     

There is no transmembrane helices in MGYG000000243_00170.