CAZyme Information: MGYG000000243_01800

Basic Information

• Species: Phocaeicola massiliensis
• Lineage: Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Bacteroidaceae; Phocaeicola; Phocaeicola massiliensis
• CAZyme ID: MGYG000000243_01800
• CAZy Family: GH3
• CAZyme Description: Beta-hexosaminidase

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
989		110550.15	9.5059

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000000243	3947824	Isolate	China	Asia

• Gene Location: Start: 102108; End: 105077 Strand: -

Full Sequence      

MKKIILLYVLCIFGLSLYAQQVPNVLRRGGADSAECREWVEKQLSEMSLKEKIGQLFIHT
VAPLNTQSNRANIYNAVKEYKVGGLLFSGGQVGNQAILTNYAQELSKVPLFIAFDGEWGL
AMRLKNTPRFPRNRVLGCIQDNQLLYEYGKEVARQCKEIGVQINFAPVADVDINPRNPVI
NTRSFGGDPKNVAQKVVAYSRGLEDGGVLSVAKHFPGHGDTEVDSHKALPVLNFDRARLD
SIELYPFKEVIHAGLGGIMAGHLEVPELGKNPASLSAHVIDNLLCRELGFRGLVFTDALE
MKGVSQNENLCAKALKAGNDLLLAPRNLKRELNGVLEAVKSGWLSEKEITEKCRKVLTYK
YALGLKTKPHVQISGLDKRLNRTEVAELISRLQKAAITVVNNTGGVLPLDAKLRGTTVLN
IGKPSSGLEFYSRLKQYLPLRRIMATSDSMSAIRKELVNSQRVIVVINSNDYNKYKPMLE
KLPDSLPVVYVYLMPLKTMSDMEVCWKKAAAVVLGHTDELEIQRQVADVMAGRAVADGRL
SVAVSDLFKPGDGVTMSPKVSRIYKPEDYGMSSAVLKGIDEIALEGIKAKAYPGCQILIL
KDGVPVYDKCFGTFTYEKSKEVEADNLYDLASLTKTTATLLAVMKLYDNGKFGLTDRVSD
YIPVLKGTGKERITIEELLMHQSGLPAFWPFYKKAIDEESYTGAFYRTRPDAGHHRQIDT
RLFVIDHFGYKKELVSKVFSSDYPLLVSDSMYLHRSFRDSVLQQIAGVPLKDRRYRYSDL
NFILLREIVENITKIPMNIFLQKEFYKPMKMEHTLFLPLRRFAKDEIVPTVKADYLRKGG
MLQGYVHDESAAFLGGVSGNAGLFSTAGDVAKVYQMLIDGGVYEGVRYLSKETCDLFLTH
TSKISRRGLGFDKPDKKNEAKSPCAAEAPAEVVGHTGFTGTCAWADPKNKLVFVFLSNRI
YPRPFDHKQLMRLNIRPRIQQVMYQAMTK

Enzyme Prediction     

EC	3.2.1.-

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GH3	104	323	6.7e-56	0.9722222222222222

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
COG1472	BglX	7.95e-81	47	412	1	353	Periplasmic beta-glucosidase and related glycosidases [Carbohydrate transport and metabolism].
pfam00933	Glyco_hydro_3	4.43e-71	48	358	1	315	Glycosyl hydrolase family 3 N terminal domain.
PRK03642	PRK03642	3.35e-59	566	959	30	395	putative periplasmic esterase; Provisional
pfam00144	Beta-lactamase	4.42e-43	579	973	1	322	Beta-lactamase. This family appears to be distantly related to pfam00905 and PF00768 D-alanyl-D-alanine carboxypeptidase.
COG1680	AmpC	2.99e-40	570	970	32	374	CubicO group peptidase, beta-lactamase class C family [Defense mechanisms].

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
QEW35592.1	0.0	1	989	1	992
QQY43102.1	0.0	1	989	1	992
QQY39794.1	0.0	1	989	1	992
ABR37873.1	0.0	1	989	1	992
QUT56867.1	0.0	1	989	1	992

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
6K5J_A	6.65e-60	47	404	11	380	Structureof a glycoside hydrolase family 3 beta-N-acetylglucosaminidase from Paenibacillus sp. str. FPU-7 [Paenibacillaceae]
3BMX_A	4.79e-53	44	419	39	456	Beta-N-hexosaminidase(YbbD) from Bacillus subtilis [Bacillus subtilis],3BMX_B Beta-N-hexosaminidase (YbbD) from Bacillus subtilis [Bacillus subtilis],3NVD_A Structure of YBBD in complex with pugnac [Bacillus subtilis],3NVD_B Structure of YBBD in complex with pugnac [Bacillus subtilis]
3LK6_A	1.43e-52	44	419	13	430	ChainA, Lipoprotein ybbD [Bacillus subtilis],3LK6_B Chain B, Lipoprotein ybbD [Bacillus subtilis],3LK6_C Chain C, Lipoprotein ybbD [Bacillus subtilis],3LK6_D Chain D, Lipoprotein ybbD [Bacillus subtilis]
4GYJ_A	2.37e-52	44	419	43	460	Crystalstructure of mutant (D318N) bacillus subtilis family 3 glycoside hydrolase (nagz) in complex with glcnac-murnac (space group P1) [Bacillus subtilis subsp. subtilis str. 168],4GYJ_B Crystal structure of mutant (D318N) bacillus subtilis family 3 glycoside hydrolase (nagz) in complex with glcnac-murnac (space group P1) [Bacillus subtilis subsp. subtilis str. 168],4GYK_A Crystal structure of mutant (D318N) bacillus subtilis family 3 glycoside hydrolase (nagz) in complex with glcnac-murnac (space group P1211) [Bacillus subtilis subsp. subtilis str. 168],4GYK_B Crystal structure of mutant (D318N) bacillus subtilis family 3 glycoside hydrolase (nagz) in complex with glcnac-murnac (space group P1211) [Bacillus subtilis subsp. subtilis str. 168]
3SQL_A	9.55e-52	79	394	55	381	CrystalStructure of Glycoside Hydrolase from Synechococcus [Synechococcus sp. PCC 7002],3SQL_B Crystal Structure of Glycoside Hydrolase from Synechococcus [Synechococcus sp. PCC 7002],3SQM_A Crystal Structure of Glycoside Hydrolase from Synechococcus Complexed with N-acetyl-D-glucosamine [Synechococcus sp. PCC 7002],3SQM_B Crystal Structure of Glycoside Hydrolase from Synechococcus Complexed with N-acetyl-D-glucosamine [Synechococcus sp. PCC 7002],3SQM_C Crystal Structure of Glycoside Hydrolase from Synechococcus Complexed with N-acetyl-D-glucosamine [Synechococcus sp. PCC 7002],3SQM_D Crystal Structure of Glycoside Hydrolase from Synechococcus Complexed with N-acetyl-D-glucosamine [Synechococcus sp. PCC 7002]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
P40406	2.62e-52	44	419	39	456	Beta-hexosaminidase OS=Bacillus subtilis (strain 168) OX=224308 GN=nagZ PE=1 SV=1
P48823	9.10e-44	77	409	59	425	Beta-hexosaminidase A OS=Pseudoalteromonas piscicida OX=43662 GN=cht60 PE=1 SV=1
O05213	5.68e-40	543	969	19	414	N-acetylmuramyl-L-alanine amidase OS=Bacillus subtilis (strain 168) OX=224308 GN=amiE PE=1 SV=3
P77619	3.02e-39	566	959	30	395	Putative D-alanyl-D-alanine carboxypeptidase OS=Escherichia coli (strain K12) OX=83333 GN=yfeW PE=1 SV=2
Q8XBJ0	3.35e-38	566	959	30	395	Putative D-alanyl-D-alanine carboxypeptidase OS=Escherichia coli O157:H7 OX=83334 GN=yfeW PE=3 SV=1

SignalP and Lipop Annotations

This protein is predicted as SP

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
0.000233	0.999169	0.000158	0.000148	0.000141	0.000140

TMHMM  Annotations     

start	end
5	22