CAZyme Information: MGYG000000245_01632

Basic Information

• Species: Roseburia sp003470905
• Lineage: Bacteria; Firmicutes_A; Clostridia; Lachnospirales; Lachnospiraceae; Roseburia; Roseburia sp003470905
• CAZyme ID: MGYG000000245_01632
• CAZy Family: GH2
• CAZyme Description: Beta-galactosidase BoGH2A

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
817	MGYG000000245_8|CGC1	93042.65	5.2691

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000000245	4145433	Isolate	China	Asia

• Gene Location: Start: 51596; End: 54049 Strand: +

Full Sequence      

MIMNKTELLLNEGWKFHFGECEEAWYQGYDDRAFERVMLPHDWAVAYPFSKQYSSGTGYL
TGGIGWYRLHFYLPEEYQGKSIRLVFDSVYKNSQVWVNSYYQGKHPYGYTGFSYDISGIA
HFGEQENVVSVKVTHTDIADSRWYTGSGITRKVTLVVEEQVHPAEYGVTFVTEKLVADGK
GLTAVQAAVSIRHEMCNQTKEQKTCVVCTKLSDPQGTPVAEWKEQVQIPAGNTVSCSMHG
TIRDPKCWSPEHPDLYGMETWYETTDEDGKKISYLADTQKVGIRVAEFDADRGFFLNGVP
MKIKGVCVHHDAGCLGAAVTKEVWHRRLAKLKGCGCNAIRCSHNPHMPELYELCDTMGFL
VMDEAFDEWENAKNKWSTGHNVYPPKHQGYFEDFPEWHEKDLRAMVRRDRNHPSIILWSI
GNEIDYPNDPYCHPSFMEMTGNNDANKPAAERQYDPAKPDMRRLLPIAEELSRIVKSEDK
SRPVTMALAYPELSAKLGILEHLDVAGYNYKEQYYEADHRDFPHIPFLGSENGHSYEAWD
AVKSNDYISGQFLWTGIDYLGEAHGWPIHGSGAGILDCAGFEKARYYRRKSLWCEEPQLY
LATRLWSEESAEWIPCQENWNYKAGEKVIVMCYSNLPETAVFINGKEAGRQIGYNDDGAY
RFEVLWEAGILEASGYDENGNIIEQERLLTTQNAAGIKAEVYQPEEIPFQETQKNGYLYQ
IALTLVDQNGQKVVWDDKKLRMAVSGAGMLAGIENGDLSDVTPYAENARKTKDGRLMIYV
RRIKKGKIRVEISETDSIAQKNCIAEKGGMHLEVEMD

Enzyme Prediction     

EC	3.2.1.23

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GH2	6	589	4.2e-112	0.5944148936170213

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
COG3250	LacZ	6.38e-63	10	564	15	507	Beta-galactosidase/beta-glucuronidase [Carbohydrate transport and metabolism].
PRK10340	ebgA	6.45e-36	65	426	111	452	cryptic beta-D-galactosidase subunit alpha; Reviewed
PRK10150	PRK10150	1.01e-29	66	425	68	419	beta-D-glucuronidase; Provisional
PRK09525	lacZ	2.68e-28	65	484	122	485	beta-galactosidase.
pfam02836	Glyco_hydro_2_C	9.28e-24	291	564	5	250	Glycosyl hydrolases family 2, TIM barrel domain. This family contains beta-galactosidase, beta-mannosidase and beta-glucuronidase activities.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
CBL08200.1	0.0	1	817	1	835
CBL12712.1	0.0	1	817	6	840
VCV23256.1	0.0	1	817	1	817
QHQ63248.1	1.46e-299	3	781	1	763
QBE95340.1	5.21e-287	3	783	1	799

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
5T9A_A	2.25e-159	11	778	33	816	Crystalstructure of BuGH2Cwt [Bacteroides uniformis],5T9A_B Crystal structure of BuGH2Cwt [Bacteroides uniformis],5T9A_C Crystal structure of BuGH2Cwt [Bacteroides uniformis],5T9A_D Crystal structure of BuGH2Cwt [Bacteroides uniformis],5T9G_A Crystal structure of BuGH2Cwt in complex with Galactoisofagomine [Bacteroides uniformis],5T9G_B Crystal structure of BuGH2Cwt in complex with Galactoisofagomine [Bacteroides uniformis],5T9G_C Crystal structure of BuGH2Cwt in complex with Galactoisofagomine [Bacteroides uniformis],5T9G_D Crystal structure of BuGH2Cwt in complex with Galactoisofagomine [Bacteroides uniformis]
5T98_A	9.21e-118	11	780	31	792	Crystalstructure of BuGH2Awt [Bacteroides uniformis],5T98_B Crystal structure of BuGH2Awt [Bacteroides uniformis],5T99_A Crystal structure of BuGH2Awt in complex with Galactoisofagomine [Bacteroides uniformis],5T99_B Crystal structure of BuGH2Awt in complex with Galactoisofagomine [Bacteroides uniformis]
4YPJ_A	1.01e-116	11	793	13	794	ChainA, Beta galactosidase [Niallia circulans],4YPJ_B Chain B, Beta galactosidase [Niallia circulans]
7CWD_A	1.68e-115	11	780	7	774	ChainA, beta-glalactosidase [Niallia circulans],7CWI_A Chain A, beta-galactosidase [Niallia circulans]
6B6L_A	3.03e-111	7	781	7	751	Thecrystal structure of glycosyl hydrolase family 2 (GH2) member from Bacteroides cellulosilyticus DSM 14838 [Bacteroides cellulosilyticus DSM 14838],6B6L_B The crystal structure of glycosyl hydrolase family 2 (GH2) member from Bacteroides cellulosilyticus DSM 14838 [Bacteroides cellulosilyticus DSM 14838]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
A7LXS9	4.50e-131	11	780	48	821	Beta-galactosidase BoGH2A OS=Bacteroides ovatus (strain ATCC 8483 / DSM 1896 / JCM 5824 / BCRC 10623 / CCUG 4943 / NCTC 11153) OX=411476 GN=BACOVA_02645 PE=1 SV=1
T2KM09	1.66e-81	5	762	46	774	Putative beta-glucuronidase OS=Formosa agariphila (strain DSM 15362 / KCTC 12365 / LMG 23005 / KMM 3901 / M-2Alg 35-1) OX=1347342 GN=BN863_22050 PE=2 SV=2
T2KPJ7	3.68e-39	30	782	75	800	Putative beta-glucuronidase OS=Formosa agariphila (strain DSM 15362 / KCTC 12365 / LMG 23005 / KMM 3901 / M-2Alg 35-1) OX=1347342 GN=BN863_21970 PE=2 SV=1
T2KN75	7.59e-39	5	705	26	699	Beta-glucuronidase OS=Formosa agariphila (strain DSM 15362 / KCTC 12365 / LMG 23005 / KMM 3901 / M-2Alg 35-1) OX=1347342 GN=BN863_22060 PE=1 SV=1
P77989	2.44e-37	3	785	1	715	Beta-galactosidase OS=Thermoanaerobacter pseudethanolicus (strain ATCC 33223 / 39E) OX=340099 GN=lacZ PE=3 SV=2

SignalP and Lipop Annotations

This protein is predicted as OTHER

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
0.999928	0.000072	0.000020	0.000000	0.000000	0.000001

TMHMM  Annotations     

There is no transmembrane helices in MGYG000000245_01632.