CAZyme Information: MGYG000000249_02543

Basic Information

• Species: Mediterraneibacter faecis
• Lineage: Bacteria; Firmicutes_A; Clostridia; Lachnospirales; Lachnospiraceae; Mediterraneibacter; Mediterraneibacter faecis
• CAZyme ID: MGYG000000249_02543
• CAZy Family: CBM48
• CAZyme Description: 1,4-alpha-glucan branching enzyme GlgB

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
641		74741.55	6.307

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000000249	3112009	Isolate	China	Asia

• Gene Location: Start: 44425; End: 46350 Strand: +

Full Sequence      

MVKKNSTKTKVQPYEIGELDHYLFGQGNHYEIYKKMGAHEVTKNGKRGVYFAVWAPHAVN
VSVIGEFNDWDETKDKMKRGEPLGIYTCFVPEAKKGDLYKFCIETQAGEKIYKADPFANY
AELRPGTASRITDISNLNWSDSKWMTAREKWDNKEEPVSIYEVHMGSWMRHPGREDEGFY
TYREFAEAITKYVKEMGYTHVELMGIAEHPFDGSWGYQVTGYYAPTSRYGTPEDFAYMVN
YLHKNNIGVILDWVPAHFPKDAHGLADFDGTPTFEYPDPRKGEHPDWGTKVFNYEKPEVS
NFLLANALFWIEHYHVDGLRVDAVASMLYLDYGREDGQWIPNKFGGNENLEAIAFFKHLN
SVVCGRNKGVMMIAEESTAWPKVTGSPEDDGLGFTLKWNMGWMHDFTEYMKLDPYFRKNA
HHMMTFSMEYAYSENYILVLSHDEVVHLKCSMLNKMPGEGWDKFRNLKVAYAFMMGHPGK
KLLFMGQEFAQLREWSEERELDWFLLAEEPHQQIQNWYKDLLHLYKKNKALYELDNDPKG
FDWINKDDIFRSIFSFTRHSKDGKKNLLFVCNFTPVDRPDYRVGVPRRKQFKLVLNSDET
KYGGSGEERPLVYKPEKQECDGQKYSFAYPLPGYGVAVFEY

Enzyme Prediction     

EC	2.4.1.18

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GH13	192	491	8e-154	0.9933554817275747
CBM48	35	119	5.9e-16	0.881578947368421

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
PRK14705	PRK14705	0.0	12	639	595	1220	glycogen branching enzyme; Provisional
PRK14706	PRK14706	0.0	36	641	27	621	glycogen branching enzyme; Provisional
PRK05402	PRK05402	0.0	16	640	100	722	1,4-alpha-glucan branching protein GlgB.
COG0296	GlgB	0.0	16	641	6	628	1,4-alpha-glucan branching enzyme [Carbohydrate transport and metabolism].
PRK12313	PRK12313	0.0	18	641	9	627	1,4-alpha-glucan branching protein GlgB.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
CBL27570.1	0.0	1	641	1	641
QHB24149.1	0.0	1	641	1	641
QEI31650.1	0.0	1	641	1	641
QRT30382.1	0.0	1	641	1	641
QEK19514.1	0.0	14	641	9	636

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
5GQW_A	2.93e-229	13	605	127	737	Crystalstructure of branching enzyme W610N mutant from Cyanothece sp. ATCC 51142 [Crocosphaera subtropica ATCC 51142],5GQX_A Crystal structure of branching enzyme W610N mutant from Cyanothece sp. ATCC 51142 in complex with maltoheptaose [Crocosphaera subtropica ATCC 51142]
5GR2_A	4.14e-229	13	605	127	737	Crystalstructure of branching enzyme L541A mutant from Cyanothece sp. ATCC 51142 [Crocosphaera subtropica ATCC 51142],5GR4_A Crystal structure of branching enzyme L541A mutant from Cyanothece sp. ATCC 51142 in complex with maltoheptaose [Crocosphaera subtropica ATCC 51142]
5GR5_A	4.14e-229	13	605	127	737	Crystalstructure of branching enzyme W610A mutant from Cyanothece sp. ATCC 51142 [Crocosphaera subtropica ATCC 51142]
5GQZ_A	5.87e-229	13	605	127	737	Crystalstructure of branching enzyme Y500A mutant from Cyanothece sp. ATCC 51142 [Crocosphaera subtropica ATCC 51142]
5GQU_A	8.31e-229	13	605	127	737	Crystalstructure of branching enzyme from Cyanothece sp. ATCC 51142 [Crocosphaera subtropica ATCC 51142],5GQV_A Crystal structure of branching enzyme from Cyanothece sp. ATCC 51142 in complex with maltohexaose [Crocosphaera subtropica ATCC 51142],5GQY_A Crystal structure of branching enzyme from Cyanothece sp. ATCC 51142 in complex with maltoheptaose [Crocosphaera subtropica ATCC 51142]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
P30539	1.29e-303	16	641	7	632	1,4-alpha-glucan branching enzyme GlgB OS=Butyrivibrio fibrisolvens OX=831 GN=glgB PE=1 SV=1
Q8DLB8	3.23e-242	15	605	105	714	1,4-alpha-glucan branching enzyme GlgB OS=Thermosynechococcus vestitus (strain IAM M-273 / NIES-2133 / BP-1) OX=197221 GN=glgB PE=3 SV=1
Q7NL20	3.46e-241	16	640	103	727	1,4-alpha-glucan branching enzyme GlgB OS=Gloeobacter violaceus (strain ATCC 29082 / PCC 7421) OX=251221 GN=glgB PE=3 SV=1
B8CVY1	3.01e-239	18	640	8	626	1,4-alpha-glucan branching enzyme GlgB OS=Halothermothrix orenii (strain H 168 / OCM 544 / DSM 9562) OX=373903 GN=glgB PE=3 SV=1
Q2JK68	8.83e-239	16	606	117	718	1,4-alpha-glucan branching enzyme GlgB OS=Synechococcus sp. (strain JA-2-3B'a(2-13)) OX=321332 GN=glgB PE=3 SV=1

SignalP and Lipop Annotations

This protein is predicted as OTHER

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
1.000031	0.000000	0.000000	0.000000	0.000000	0.000000

TMHMM  Annotations     

There is no transmembrane helices in MGYG000000249_02543.