CAZyme Information: MGYG000000250_01637

Basic Information

• Species: TF01-11 sp001414325
• Lineage: Bacteria; Firmicutes_A; Clostridia; Lachnospirales; Lachnospiraceae; TF01-11; TF01-11 sp001414325
• CAZyme ID: MGYG000000250_01637
• CAZy Family: GH2
• CAZyme Description: Beta-galactosidase

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
966		108828.09	4.6832

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000000250	3613289	Isolate	China	Asia

• Gene Location: Start: 218621; End: 221521 Strand: -

Full Sequence      

MIKKIDLSGIWNFELDPDKKGIEARLFDKTKNDTITLPGTTSIAKKGTFSDKKETGFLTD
PYLFEGYAWYSKDVHIDESDTDKNIFLFLERTRLTKIWIDDTFAGEFDSLTTPHYYDITR
FVKKTDFRITVLVSNVNYPTKGGHLTSPDTQTNWNGIIGEISLRIFDKIYISDVKTYPDV
DKKSVTVILKTVNDTAEDKKVTLNVNAFLTDINGDTGIKAPAAEIQVIFAKGESTAEFEY
FLGEDAVLWSEYTPVIYRLEISLCGSDEKTVTTFGLRKFSTTETKFLINGEPAFLRGKHD
GLVFPLTGAVPADVDEWVRVMKISKSYGINHYRYHTCCPPEAAFIAADLLGIYMEPQLPF
WGTLTAPGDENHNETEQNYLIEEGFRMLNTFGNHASYCMMSLGNELWGSKERMAEIITGY
RNVDNRHLYTQGSNNFQHTPVLLPEDDFFVGVRFSKNRLIRGSYGMCDAPLGHIQWEEPS
TTHNYDKEIVPDDENSGNGTDAGEEIEIQYGTGVKKVKAASADGPLIPHIPVVTHEIGQY
AVYPNFKEIPKYTGVLKARNFEVFRDNLDKKGMLDQADDFFYASGMLSVQCYKEELEAAA
RSSLVAGYQILDLQDFPGQGTALVGILDAFMDSKGHITPEEWAGFCSDAILLAQFEKYVY
TSGENFKADLAIRYYNPEKLTGKKLHYELIRRDVSAAETTGFAVNGETSVVTSGDVKIPD
GAKELVQLGGISFAMPETDKTAVYDLILTIDGTPYKNKYELYCYPVNDDVLSLENIASIG
EGDNQVFITNEFTETKSLLKQGKKVLFMPNEVKESLEGFYCTDFWCYPMFRDICEWMKKP
VAVGTMGLLIDSSHPALNDFATHRYSTPQWYKIVSHSRCAILDDVTDKDYRPIVQMADNF
ERNHKLGILFEGKAGDGKLMVCTSKLGEIAESTEVKQFIKSLVNYLTSDVFAPEKELDLE
KLETIF

Enzyme Prediction     

No EC number prediction in MGYG000000250_01637.

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GH2	3	445	6.9e-60	0.4867021276595745

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
COG3250	LacZ	1.99e-24	1	637	9	623	Beta-galactosidase/beta-glucuronidase [Carbohydrate transport and metabolism].
PRK10150	PRK10150	6.11e-17	6	300	14	301	beta-D-glucuronidase; Provisional
pfam02837	Glyco_hydro_2_N	9.70e-09	7	135	4	135	Glycosyl hydrolases family 2, sugar binding domain. This family contains beta-galactosidase, beta-mannosidase and beta-glucuronidase activities and has a jelly-roll fold. The domain binds the sugar moiety during the sugar-hydrolysis reaction.
PRK10340	ebgA	1.05e-07	116	405	160	449	cryptic beta-D-galactosidase subunit alpha; Reviewed
pfam00703	Glyco_hydro_2	3.92e-07	169	277	1	106	Glycosyl hydrolases family 2. This family contains beta-galactosidase, beta-mannosidase and beta-glucuronidase activities.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
CBL16908.1	0.0	1	966	1	930
BAV13119.1	0.0	1	965	1	932
ADL53165.1	0.0	1	965	1	932
QAA34542.1	0.0	1	965	1	932
QKS45132.1	0.0	1	966	1	942

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
6U7J_A	1.48e-15	6	411	23	423	UnculturedClostridium sp. Beta-glucuronidase [uncultured Clostridium sp.],6U7J_B Uncultured Clostridium sp. Beta-glucuronidase [uncultured Clostridium sp.],6U7J_C Uncultured Clostridium sp. Beta-glucuronidase [uncultured Clostridium sp.],6U7J_D Uncultured Clostridium sp. Beta-glucuronidase [uncultured Clostridium sp.]
6D4O_A	1.55e-15	6	405	17	428	Eubacteriumeligens beta-glucuronidase bound to an amoxapine-glucuronide conjugate [Lachnospira eligens]
6BO6_A	6.14e-15	6	405	14	425	Eubacteriumeligens beta-glucuronidase bound to UNC4917 glucuronic acid conjugate [[Eubacterium] eligens ATCC 27750]
6BJQ_A	6.41e-15	6	405	38	449	ChainA, Glycoside Hydrolase Family 2 candidate b-glucuronidase [[Eubacterium] eligens ATCC 27750]
6BJW_A	6.42e-15	6	405	38	449	ChainA, Glycoside Hydrolase Family 2 candidate b-glucuronidase [[Eubacterium] eligens ATCC 27750]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
P77989	1.04e-14	27	382	27	368	Beta-galactosidase OS=Thermoanaerobacter pseudethanolicus (strain ATCC 33223 / 39E) OX=340099 GN=lacZ PE=3 SV=2
O07684	5.35e-10	4	414	42	477	Beta-galactosidase large subunit OS=Lactobacillus acidophilus (strain ATCC 700396 / NCK56 / N2 / NCFM) OX=272621 GN=lacL PE=3 SV=2
Q48846	3.87e-07	97	405	161	465	Beta-galactosidase large subunit OS=Latilactobacillus sakei OX=1599 GN=lacL PE=3 SV=1
O77695	4.65e-06	6	405	37	448	Beta-glucuronidase (Fragment) OS=Chlorocebus aethiops OX=9534 GN=GUSB PE=2 SV=1

SignalP and Lipop Annotations

This protein is predicted as OTHER

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
1.000054	0.000003	0.000000	0.000000	0.000000	0.000000

TMHMM  Annotations     

There is no transmembrane helices in MGYG000000250_01637.