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CAZyme Information: MGYG000000277_00856

You are here: Home > Sequence: MGYG000000277_00856

Basic Information | Genomic context | Full Sequence | Enzyme annotations |  CAZy signature domains |  CDD domains | CAZyme hits | PDB hits | Swiss-Prot hits | SignalP and Lipop annotations | TMHMM annotations

Basic Information help

Species Streptococcus mutans
Lineage Bacteria; Firmicutes; Bacilli; Lactobacillales; Streptococcaceae; Streptococcus; Streptococcus mutans
CAZyme ID MGYG000000277_00856
CAZy Family GH68
CAZyme Description Inulosucrase
CAZyme Property
Protein Length CGC Molecular Weight Isoelectric Point
795 MGYG000000277_2|CGC4 87320.7 4.9654
Genome Property
Genome Assembly ID Genome Size Genome Type Country Continent
MGYG000000277 2020595 Isolate China Asia
Gene Location Start: 193558;  End: 195945  Strand: -

Full Sequence      Download help

Enzyme Prediction      help

EC 2.4.1.10 2.4.1.9

CAZyme Signature Domains help

Family Start End Evalue family coverage
GH68 189 636 2.4e-146 0.9928057553956835

CDD Domains      download full data without filtering help

Cdd ID Domain E-Value qStart qEnd sStart sEnd Domain Description
pfam02435 Glyco_hydro_68 1.73e-180 189 628 1 411
Levansucrase/Invertase. This Pfam family consists of the glycosyl hydrolase 68 family, including several bacterial levansucrase enzymes, and invertase from zymomonas.
cd08997 GH68 5.11e-107 244 624 1 352
Glycosyl hydrolase family 68, includes levansucrase, beta-fructofuranosidase and inulosucrase. Glycosyl hydrolase family 68 (GH68) consists of frucosyltransferases (FTFs) that include levansucrase (EC 2.4.1.10), beta-fructofuranosidase (EC 3.2.1.26) and inulosucrase (EC 2.4.1.9), all of which use sucrose as their preferential donor substrate. Levansucrase, also known as beta-D-fructofuranosyl transferase, catalyzes the transfer of the sucrose fructosyl moiety to a growing levan chain. Similarly, inulosucrase catalyzes long inulin-type of fructans, and beta-fructofuranosidases create fructooligosaccharides (FOS). However, in the absence of high fructan/sucrose ratio, some GH68 enzymes can also use fructan as donor substrate. GH68 retaining enzymes (i.e. they retain the configuration at anomeric carbon atom of the substrate) catalyze hydrolysis in two steps involving a covalent glycosyl enzyme intermediate: an aspartate located close to the N-terminus acts as the catalytic nucleophile and a glutamate acts as the general acid/base; a conserved aspartate residue in the Arg-Asp-Pro (RDP) motif stabilizes the transition state. A common structural feature of all these enzymes is a 5-bladed beta-propeller domain, similar to GH43, that contains the catalytic acid and catalytic base. A long V-shaped groove, partially enclosed at one end, forms a single extended substrate-binding surface across the face of the propeller. Biotechnological applications of these enzymes include use of inulin in inexpensive production of rich fructose syrups as well as use of FOS as health-promoting pre-biotics.
cd08979 GH_J 2.06e-27 245 616 1 291
Glycosyl hydrolase families 32 and 68, which form the clan GH-J. This glycosyl hydrolase family clan J (according to carbohydrate-active enzymes database (CAZY)) includes family 32 (GH32) and 68 (GH68). GH32 enzymes include invertase (EC 3.2.1.26) and other other fructofuranosidases such as inulinase (EC 3.2.1.7), exo-inulinase (EC 3.2.1.80), levanase (EC 3.2.1.65), and transfructosidases such sucrose:sucrose 1-fructosyltransferase (EC 2.4.1.99), fructan:fructan 1-fructosyltransferase (EC 2.4.1.100), sucrose:fructan 6-fructosyltransferase (EC 2.4.1.10), fructan:fructan 6G-fructosyltransferase (EC 2.4.1.243) and levan fructosyltransferases (EC 2.4.1.-). The GH68 family consists of frucosyltransferases (FTFs) that include levansucrase (EC 2.4.1.10, also known as beta-D-fructofuranosyl transferase), beta-fructofuranosidase (EC 3.2.1.26) and inulosucrase (EC 2.4.1.9). GH32 and GH68 family enzymes are retaining enzymes (i.e. they retain the configuration at anomeric carbon atom of the substrate) and catalyze hydrolysis in two steps involving a covalent glycosyl enzyme intermediate: an aspartate located close to the N-terminus acts as the catalytic nucleophile and a glutamate acts as the general acid/base; a conserved aspartate residue in the Arg-Asp-Pro (RDP) motif stabilizes the transition state. A common structural feature of all these enzymes is a 5-bladed beta-propeller domain, similar to GH43, that contains the catalytic acid and catalytic base. A long V-shaped groove, partially enclosed at one end, forms a single extended substrate-binding surface across the face of the propeller.
NF033875 Agg_substance 3.57e-07 1 198 5 184
LPXTG-anchored aggregation substance. Aggregation substances, as described in Enterococcus, are LPXTG-anchored large surface proteins that contribute to virulence. Several closely related paralogs may be found in a single strain.
TIGR03715 KxYKxGKxW 1.70e-06 4 26 1 23
KxYKxGKxW signal peptide. This model describes a novel form of signal peptide that occurs as an N-terminal domain with a recognizable motif, reminiscent of the YSIRK and PEP-CTERM forms of signal peptide. This domain tends to occur on long, low-complexity (usually Serine-rich and heavily glycosylated) proteins of the Firmicutes, and (as with YSIRK) the majority of these proteins have the LPXTG cell wall-anchoring motif at the C-terminus.

CAZyme Hits      help

Hit ID E-Value Query Start Query End Hit Start Hit End
ARS63409.1 0.0 1 795 1 795
QIR04803.1 0.0 1 795 1 795
QIR02672.1 0.0 1 795 1 795
QIQ94780.1 0.0 1 795 1 795
QIR01018.1 0.0 1 795 1 795

PDB Hits      download full data without filtering help

Hit ID E-Value Query Start Query End Hit Start Hit End Description
2YFR_A 6.60e-248 156 677 39 564
Crystalstructure of inulosucrase from Lactobacillus johnsonii NCC533 [Lactobacillus johnsonii],2YFT_A Crystal structure of inulosucrase from Lactobacillus johnsonii NCC533 in complex with 1-kestose [Lactobacillus johnsonii]
2YFS_A 3.77e-247 156 677 39 564
Crystalstructure of inulosucrase from Lactobacillus johnsonii NCC533 in complex with sucrose [Lactobacillus johnsonii]
3OM7_A 7.25e-87 211 640 33 455
ChainA, Levansucrase [Priestia megaterium],3OM7_B Chain B, Levansucrase [Priestia megaterium],3OM7_C Chain C, Levansucrase [Priestia megaterium],3OM7_D Chain D, Levansucrase [Priestia megaterium]
3OM4_A 7.25e-87 211 640 33 455
ChainA, Levansucrase [Priestia megaterium],3OM4_B Chain B, Levansucrase [Priestia megaterium],3OM4_C Chain C, Levansucrase [Priestia megaterium],3OM4_D Chain D, Levansucrase [Priestia megaterium]
6PWQ_A 1.88e-86 209 654 30 458
Crystalstructure of Levansucrase from Bacillus subtilis mutant S164A at 2.6 A [Bacillus subtilis],6PWQ_B Crystal structure of Levansucrase from Bacillus subtilis mutant S164A at 2.6 A [Bacillus subtilis]

Swiss-Prot Hits      download full data without filtering help

Hit ID E-Value Query Start Query End Hit Start Hit End Description
P11701 0.0 1 795 1 795
Levansucrase OS=Streptococcus mutans serotype c (strain ATCC 700610 / UA159) OX=210007 GN=ftf PE=3 SV=2
Q74K42 2.85e-245 1 686 2 716
Inulosucrase OS=Lactobacillus johnsonii (strain CNCM I-12250 / La1 / NCC 533) OX=257314 GN=inuJ PE=1 SV=1
D3WYV9 9.65e-245 156 679 176 703
Inulosucrase OS=Lactobacillus gasseri OX=1596 GN=inuGB PE=1 SV=1
D3WYW0 2.84e-213 158 679 163 690
Levansucrase OS=Lactobacillus gasseri OX=1596 GN=levG PE=1 SV=1
Q70XJ9 2.78e-205 164 689 230 764
Levansucrase OS=Fructilactobacillus sanfranciscensis OX=1625 GN=levS PE=1 SV=1

SignalP and Lipop Annotations help

This protein is predicted as SP

Other SP_Sec_SPI LIPO_Sec_SPII TAT_Tat_SPI TATLIP_Sec_SPII PILIN_Sec_SPIII
0.002123 0.994168 0.003018 0.000241 0.000213 0.000209

TMHMM  Annotations      download full data without filtering help

start end
13 35
777 794