CAZyme Information: MGYG000000282_00069

Basic Information

• Species: TF01-11 sp003524945
• Lineage: Bacteria; Firmicutes_A; Clostridia; Lachnospirales; Lachnospiraceae; TF01-11; TF01-11 sp003524945
• CAZyme ID: MGYG000000282_00069
• CAZy Family: CBM48
• CAZyme Description: 1,4-alpha-glucan branching enzyme GlgB

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
760	MGYG000000282_1|CGC1	87480.19	5.2391

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000000282	3383470	Isolate	China	Asia

• Gene Location: Start: 66745; End: 69027 Strand: +

Full Sequence      

MNKRLYDLMDWEAIEGIVYSEEDHPENILGPHKIRGGFLVQTFIPGAQEVTLQLRKSGKT
IAMELADEAGFFAAILPEKKPVSYFYNVVYDNGDSEQREDPYLYPDLLGANELKRFESGI
HYSVYDYLGSHTMAVYGCGSQAEPEWDVRTVKKDGVFGTHFAVWAPNAMRVSVVGDFNHW
DGRVHPMCRIGDSGVFALFIPGVDQGAVYKYEIKVNYKTLLLKTDPYGTYCEERPANASI
VCNTTGYKWKDKKWMDARKKDSFENEAVSIYEVHLGSWMKKEWDGEGELTCDKEFYNYRE
LAPLLADYVKKMHYTHVELMPVMEHPLDESWGYQVTDYYAVTSRYGSPEDFMYFIDHLHQ
QGIGVILDWVPAHFPKDENGLARFDGTCLYEHLDPRQGEHPHWGTLIYNYGRPQVANFLI
ANALFWVKRYHADGIRMDAVASMLYLDYGKKDGEWVANMYGSNENLEAIELLKNLSEIFK
KECPGAWLIAEESTAWPQVTTRASEGGLGFDMKWNMGWMNDFIAYMQTDPLFRKGRHGML
TFSMIYAYSEKFILVLSHDEVVHLKGSMLAKMPGDEDQKFANLRAAYGFMMMHPGKKLLF
MGQEFGQTTEWNEKQSLPWDEAEKPEHRKLQKFVAGLNEFYQAHPALYEMDYDEDGFEWL
SSMDADHSIITFMRYSAQKKERLLVVCNFTPVLYENFKVGVPEAGKYKEIFNSDAEVYGG
SGHGNPRQKRAKKVAWDGKDYSISIEVPPLGVCVFRYIES

Enzyme Prediction     

EC	2.4.1.18

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GH13	307	607	6e-155	0.9966777408637874
CBM48	155	230	3.8e-16	0.7894736842105263

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
PRK12313	PRK12313	0.0	107	757	6	627	1,4-alpha-glucan branching protein GlgB.
COG0296	GlgB	0.0	103	757	1	628	1,4-alpha-glucan branching enzyme [Carbohydrate transport and metabolism].
PRK14705	PRK14705	0.0	9	755	503	1220	glycogen branching enzyme; Provisional
PRK14706	PRK14706	0.0	151	758	32	622	glycogen branching enzyme; Provisional
PRK05402	PRK05402	0.0	12	756	4	722	1,4-alpha-glucan branching protein GlgB.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
QHQ61835.1	0.0	1	755	1	731
BCJ99479.1	0.0	1	760	1	735
CBK76766.1	0.0	1	755	1	740
CBL36572.1	0.0	1	755	1	740
AWY97839.1	0.0	1	759	1	734

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
5GQW_A	3.20e-242	12	756	28	772	Crystalstructure of branching enzyme W610N mutant from Cyanothece sp. ATCC 51142 [Crocosphaera subtropica ATCC 51142],5GQX_A Crystal structure of branching enzyme W610N mutant from Cyanothece sp. ATCC 51142 in complex with maltoheptaose [Crocosphaera subtropica ATCC 51142]
5GQZ_A	3.20e-242	12	756	28	772	Crystalstructure of branching enzyme Y500A mutant from Cyanothece sp. ATCC 51142 [Crocosphaera subtropica ATCC 51142]
5GR5_A	4.53e-242	12	756	28	772	Crystalstructure of branching enzyme W610A mutant from Cyanothece sp. ATCC 51142 [Crocosphaera subtropica ATCC 51142]
5GQU_A	9.09e-242	12	756	28	772	Crystalstructure of branching enzyme from Cyanothece sp. ATCC 51142 [Crocosphaera subtropica ATCC 51142],5GQV_A Crystal structure of branching enzyme from Cyanothece sp. ATCC 51142 in complex with maltohexaose [Crocosphaera subtropica ATCC 51142],5GQY_A Crystal structure of branching enzyme from Cyanothece sp. ATCC 51142 in complex with maltoheptaose [Crocosphaera subtropica ATCC 51142]
5GR1_A	9.09e-242	12	756	28	772	Crystalstructure of branching enzyme Y500A/D501A mutant from Cyanothece sp. ATCC 51142 in complex with maltoheptaose [Crocosphaera subtropica ATCC 51142],5GR6_A Crystal structure of branching enzyme Y500A/D501A double mutant from Cyanothece sp. ATCC 51142 [Crocosphaera subtropica ATCC 51142]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
Q1AZ86	1.28e-259	12	756	5	718	1,4-alpha-glucan branching enzyme GlgB OS=Rubrobacter xylanophilus (strain DSM 9941 / NBRC 16129 / PRD-1) OX=266117 GN=glgB PE=3 SV=1
Q8DLB8	1.35e-257	12	756	6	749	1,4-alpha-glucan branching enzyme GlgB OS=Thermosynechococcus vestitus (strain IAM M-273 / NIES-2133 / BP-1) OX=197221 GN=glgB PE=3 SV=1
Q2JT08	7.12e-256	8	757	4	753	1,4-alpha-glucan branching enzyme GlgB OS=Synechococcus sp. (strain JA-3-3Ab) OX=321327 GN=glgB PE=3 SV=1
Q3JCN0	1.98e-255	12	756	17	731	1,4-alpha-glucan branching enzyme GlgB OS=Nitrosococcus oceani (strain ATCC 19707 / BCRC 17464 / JCM 30415 / NCIMB 11848 / C-107) OX=323261 GN=glgB PE=3 SV=1
Q7NL20	4.60e-255	14	756	10	727	1,4-alpha-glucan branching enzyme GlgB OS=Gloeobacter violaceus (strain ATCC 29082 / PCC 7421) OX=251221 GN=glgB PE=3 SV=1

SignalP and Lipop Annotations

This protein is predicted as OTHER

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
1.000040	0.000000	0.000000	0.000000	0.000000	0.000000

TMHMM  Annotations     

There is no transmembrane helices in MGYG000000282_00069.