CAZyme Information: MGYG000000287_03298

Basic Information

• Species: Robinsoniella sp900539655
• Lineage: Bacteria; Firmicutes_A; Clostridia; Lachnospirales; Lachnospiraceae; Robinsoniella; Robinsoniella sp900539655
• CAZyme ID: MGYG000000287_03298
• CAZy Family: GH84
• CAZyme Description: hypothetical protein

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
1989		220017.78	4.7514

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000000287	7249439	MAG	Sweden	Europe

• Gene Location: Start: 4885; End: 10854 Strand: +

Full Sequence      

MKRLRKALSFLLIIVLTMTSVQLSGISETLHASAAENAAYEIYPTPHDVEYANGSLTLSN
EVNVVFGEGIDQATKDKVDDVLKTASVTHVVTDASAEGKTNLLVGIHKSEGAKDAADQFA
ADHTAVTEDHFNQTDAYVLTAQDNTIAAVGKDTNAAFYALSTLKMVLRQITGKQLQNFTV
TDFSDTGLRGFIEGYYGLPWSNEDRMNLMKFGGEIKCNAYVFAPKDDPYHNSKWREMYPE
EKLAEIREMAEVGRESKCDFVWTIHPFMSDRITESNYDDSLATIKKKFQQLYDAGVRRFG
VLADDAGGVSVDLIAKLLNDLTDWCKSKNDVKDILYCPPEYNWAFAGYSWTNLRNANRLV
NDDVDFFWTGQSVCGHVTKETVDGFVNGLTSDEKAGRKPLFWLNWPVNDINKVRMLMGKG
EMLNTDVTNLAGVITNPMQQSEPSKVAIFAVADYAWNIQGFNRDKSWADSFKYVEPDASE
ALHTMAKHLSDPAPNGHGLVLGESEELAPLLEEFMTLYQSGASIQEKGSLLADEFQKIAD
AADVFDRDSQNENLKSQLSPWTDSLRYLAKAAINYINTAIAYEKNDYENVWSYFSEASSY
YGLSKQCPVVNIDAVPNVEAGAKRLIPFVKNMSDVLGPKVNSVVNPNADKIRPFVPVDAE
LMKSDGINGTYNEYTEGKATDKDDNTFAWFKTTPNDSFPAGGWLGLDLGREVTLGTIKFL
QGGTTTAQSDLISNAVLEYSLDGENYTELESFKDKRDIYFNAEELGVKARYVRVRAKAAT
GKWLAVREFAVSEGEVLPSTVAYTNVASLSRRSVTATADTSVLDVNDRDITLSKNQYLGI
MLPRIRNVIEVNADYTQQNKLTMEASVNGTEWQTIDPGNLKGGLDVRYIRLINKSNDPVT
FQLSGFVIKSKEIAAPHYMESNIPASGITGADQAVDGDRSTKANFSVAQKEGQYIIYDLG
QTIPLKKLKMVQHDSEVDFIRNGKVSISADLENWTDVLTIGSSDKDPGQVTIDECFPDHE
ISYYTLSTSEPVNQETRYVKISVTKDYSARWIRFNELEINDNEYIPVENNPSFVTSDVEE
KGHRPSYLTDNNVSTTYRSSKKEGEGYLTYRVSGNTKVTAITILQSPNTISDADVSIRYG
KDQWKSIGTLGKSLNVFEDLGQDVNNVFEIKISWTHSAPELHEMYLVTADPDPVTPADKS
SLIEKYTQALAIDGKYYTKETYAVLKNAIDDAEGVIGYAEASQEDVEAALAAITDALDKL
EDIPADRTELQEAVAKAKELDAKLYTEDSYKVVTQAIAAAEESLEKDGVKQKELDEKLAE
LAEAVNALVIKGTLTKINSSELSATAGSQEGSGTDGNADQAVDGNETTYWHSNWSGGAAA
KPDIPNNIRNEFTIDVGKNRILRKLEYVPRSNNINGRILGYKLYYSPTENGDDFIEVPGG
AGTWSDNADKKEVVFNTVNARRIQIRATSTRGDSGNDKFISAAEFYVYEIIPDEEEQTCH
VTFEGGEGTTGDAPESMTAKEGEQIVLPDNTFTKEGFTFLGWTDGEEVYQPGDTYTIPSY
DVIFTAQWQEEIPEIYTLSFEGGEGALGEAPVSMTGEEGAQVVLPDNTFVKEGFAFNGWN
DGNGIYQPGDTYIIPNMDKVFTAEWISEVPENEVTAYFEGGDGALGESPEPVTVLAGSSF
ILPDNSYTKEGFVFQGWNDGMNTYQPGSEYKLVQDITFTAVWGKKPIPTYVVNFNANGGN
TSTTGITVNEGGVISVLPTASRDGYQFLGWFMAPDGGTQFTSSTKITTNVTVYAHWKQIV
AVPSKVTGLKTSYNKTKSIKITWEKAANAKGYYVYRYDSRRKEWKKIKTTTSTSYKNTGL
KEGTNYTYKVKAFNQIGSEIKEGSFSTTLKTASAPAKPSLKVSQIGSGKVKITWKKTSRC
DGVEIFMKVKNKKKYTKIASKSKSASSLKKSGLKKGTIYHFRMRRYKKAGSKKIYSNYSS
VKKVKINNK

Enzyme Prediction     

No EC number prediction in MGYG000000287_03298.

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GH84	189	486	3.9e-98	0.9932203389830508
CBM32	1346	1486	6.1e-17	0.9354838709677419
CBM32	670	790	3.1e-16	0.8870967741935484

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
pfam07555	NAGidase	6.90e-123	189	484	1	292	beta-N-acetylglucosaminidase. This family has previously been described as a hyaluronidase. However, more recently it has been shown that this family has beta-N-acetylglucosaminidase activity.
pfam02838	Glyco_hydro_20b	3.25e-20	39	182	1	123	Glycosyl hydrolase family 20, domain 2. This domain has a zincin-like fold.
pfam09479	Flg_new	7.44e-15	1730	1795	1	65	Listeria-Bacteroides repeat domain (List_Bact_rpt). This model describes a conserved core region of about 43 residues, which occurs in at least two families of tandem repeats. These include 78-residue repeats which occur from 2 to 15 times in some proteins of Bacteroides forsythus ATCC 43037, and 70-residue repeats found in families of internalins of Listeria species. Single copies are found in proteins of Fibrobacter succinogenes, Geobacter sulfurreducens, and a few other bacteria.
pfam00754	F5_F8_type_C	2.34e-14	1343	1485	1	127	F5/8 type C domain. This domain is also known as the discoidin (DS) domain family.
cd00063	FN3	1.57e-10	1803	1888	1	88	Fibronectin type 3 domain; One of three types of internal repeats found in the plasma protein fibronectin. Its tenth fibronectin type III repeat contains an RGD cell recognition sequence in a flexible loop between 2 strands. Approximately 2% of all animal proteins contain the FN3 repeat; including extracellular and intracellular proteins, membrane spanning cytokine receptors, growth hormone receptors, tyrosine phosphatase receptors, and adhesion molecules. FN3-like domains are also found in bacterial glycosyl hydrolases.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
AYE33607.1	0.0	18	1336	16	1466
QAS61771.1	0.0	18	1336	16	1466
QPS12841.1	4.32e-263	35	1304	33	1431
QMW73892.1	4.32e-263	35	1304	33	1431
QQV04689.1	6.04e-263	35	1304	33	1431

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
6PWI_A	1.23e-187	40	637	34	625	Structureof CpGH84D [Clostridium perfringens ATCC 13124],6PWI_B Structure of CpGH84D [Clostridium perfringens ATCC 13124]
6PV4_A	2.33e-148	40	645	32	653	Structureof CpGH84A [Clostridium perfringens ATCC 13124],6PV4_B Structure of CpGH84A [Clostridium perfringens ATCC 13124],6PV4_C Structure of CpGH84A [Clostridium perfringens ATCC 13124],6PV4_D Structure of CpGH84A [Clostridium perfringens ATCC 13124]
5MI4_A	1.98e-75	98	570	58	518	BtGH84mutant with covalent modification by MA3 [Bacteroides thetaiotaomicron VPI-5482],5MI5_A BtGH84 mutant with covalent modification by MA3 in complex with PUGNAc [Bacteroides thetaiotaomicron VPI-5482],5MI6_A BtGH84 mutant with covalent modification by MA3 in complex with Thiamet G [Bacteroides thetaiotaomicron VPI-5482],5MI7_A BtGH84 mutant with covalent modification by MA4 in complex with PUGNAc [Bacteroides thetaiotaomicron VPI-5482]
2J4G_A	2.82e-75	98	570	46	506	Bacteroidesthetaiotaomicron GH84 O-GlcNAcase in complex with n-butyl- thiazoline inhibitor [Bacteroides thetaiotaomicron VPI-5482],2J4G_B Bacteroides thetaiotaomicron GH84 O-GlcNAcase in complex with n-butyl- thiazoline inhibitor [Bacteroides thetaiotaomicron VPI-5482]
2CHO_A	2.88e-75	98	570	47	507	Bacteroidesthetaiotaomicron hexosaminidase with O-GlcNAcase activity [Bacteroides thetaiotaomicron VPI-5482],2CHO_B Bacteroides thetaiotaomicron hexosaminidase with O-GlcNAcase activity [Bacteroides thetaiotaomicron VPI-5482]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
P26831	8.14e-248	40	1329	39	1491	Hyaluronoglucosaminidase OS=Clostridium perfringens (strain 13 / Type A) OX=195102 GN=nagH PE=1 SV=2
Q89ZI2	2.42e-74	98	570	68	528	O-GlcNAcase BT_4395 OS=Bacteroides thetaiotaomicron (strain ATCC 29148 / DSM 2079 / JCM 5827 / CCUG 10774 / NCTC 10582 / VPI-5482 / E50) OX=226186 GN=BT_4395 PE=1 SV=1
Q8XL08	5.62e-62	44	774	48	741	O-GlcNAcase NagJ OS=Clostridium perfringens (strain 13 / Type A) OX=195102 GN=nagJ PE=1 SV=1
Q0TR53	2.35e-61	44	856	48	813	O-GlcNAcase NagJ OS=Clostridium perfringens (strain ATCC 13124 / DSM 756 / JCM 1290 / NCIMB 6125 / NCTC 8237 / Type A) OX=195103 GN=nagJ PE=1 SV=1
Q8VIJ5	1.60e-26	188	470	61	348	Protein O-GlcNAcase OS=Rattus norvegicus OX=10116 GN=Oga PE=1 SV=1

SignalP and Lipop Annotations

This protein is predicted as SP

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
0.000637	0.983326	0.015382	0.000251	0.000194	0.000178

TMHMM  Annotations     

start	end
7	26