CAZyme Information: MGYG000000287_03540

Basic Information

• Species: Robinsoniella sp900539655
• Lineage: Bacteria; Firmicutes_A; Clostridia; Lachnospirales; Lachnospiraceae; Robinsoniella; Robinsoniella sp900539655
• CAZyme ID: MGYG000000287_03540
• CAZy Family: GH2
• CAZyme Description: Beta-galactosidase

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
2184	MGYG000000287_61|CGC1	239126.72	4.709

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000000287	7249439	MAG	Sweden	Europe

• Gene Location: Start: 18410; End: 24964 Strand: -

Full Sequence      

MGKRRKKLLSMALVVGMVVSNLSPYTNVLAAEPEHVSPGSAETVELSEDPGDALPPGTAK
AIEETESDTGMDSQSVTEAVTEAVSEAVPETETITETQPVETEEETVGETKTQPKEEAEA
ELTEEADNKNANAVNNVDVNGLFAEYYTTSGSGKNVKLDVLKSKGIDYNINYGDLNPKLM
LTTGQDDAAGIRWSGRIRVPETADYTFYGLVDNGIRLWVNGEQLFNYWDGNSWDVAQTSK
AVTLEAGKFYDIKVEYFDYEGGAHATLSWSNNKGLKDKATIPSSAFYLPSNYNGIYIGSI
DTSEANLKEGEDFKGDVTVNGFGLDQVERFEIVKTTGESLKTPVYADVTSKKNEEAKIVI
PNMETGTYKLKIIQSNTAVISKGLIIVKPGTDEVSTRTERPRADWERDSYVNLNGIWEFD
FDTDEEGKNAGWFKPDQEFSRSINVPFCWESSLSGISDPDYKGQAWYKKTVNVDKSWEGK
KIFLKFGAVDWKCKLWVNGEEVGEHIGGYSAFEMDVTEYMNVGEDNVVTLWVEDKGSYGD
DSYPALIGKQGRNAPCGYIHTSGIWQTVGMEARSATYLDNAKAVSDIDNATVTYDLDVTT
DKDQTLTVEYDFASTVYDMDNDVDIPTGSVVEGSQEIQVESGANSVELSPIAIANQKLWN
YDDPNLYQGTLTVKDVDGNVLDELSTYFGLRKVEAKYYNEDLGVKYIYLNDEPVYMSGLL
DQGFWEEGIYTAPSEDALKYDILAMKEAGFNMIRKHLKIEDPLQYYWCDKLGMMVWQDMP
HATAMVPSKEGGEALGRKYYEECLDAAMEMNYNHPSIVSVMLFNETWGLYAAYNDNGKNR
NVKASDGKSTAEWVEYLYNKTKGAYPNMLIEDMSPCNSDHVQPSDLNTYHMYPKSYDGTV
GDVENRVNNAYVGSENNYKFGYKQDGDPLLNSEYGGVAAYDGDYDISYCFKYMTDIQRRY
EKQSGFVYTEPFDVEYERNGILTYDRQMKIFGYDEVAYGGDMGIKDLVQETYIGIVENPI
KNVKPGAKIKTKIMAMSWTNDVPENTVVKWRFDGTDIYGNNISTDLAGTLGMDIIPYEKA
EASLSFHAPAQACVGTLTVWIEGPDGTKIAKNFTNIVVADPSSDNKEMVLDNGKGSITMK
AAVNDRKMVTTKGTGSQDYSYTLPENFNLDSLNGMKIIAEASSYKGKIGTDKNHSSYSSE
FSQTAEGRAVASDMTVSVNGVEIDTVYLPDNPRDMRGTLSLNAPYNGATSAGDFGYLVNL
SVSKEKLAEIKAAMGDSKVMKVTYAVKADAANQNGLRIYNSVYGRYAVNPTIILNPEDIE
KADQVTAVKNIKTTSGNYSAEGVLSGNSNLTVRTEEKEGYIIALANGGSKITVTNKKTNE
VIGEAAELAAGAHHVKVTLFDEQIRVFADNNPEAVINVYDRSGFTGGITVNASRTNPVSD
LVVSPESYEAVKTEVDDTVKDVEVLDDFSDADYTKRYEVMGNAWNGNVVNGALSMSASDQ
GDKIIMKDVSMSDGIYEMDIKVTNSSETHGNVGFVFRSSNYNLGPDGANGYYAGIGDRYI
QLGRMNQGWKELAKVTVPELIVGSTHTLRVAVFGSRIQIYVDDAAVPYVDINDSTYLEGG
VAVRGYRVAATLDNIHVASIPRYSSTFEKGSDEWEANGNWKTVDGAYTSGSKGAYSLIDS
NKIKDALFAADVKPGTEDSVTALLLRAVSSSTGISGYQLVADAKNDKVQIVKSEKGKTTV
LAETGMRLQAGKTYRLTVEMQGSVIKAYKNDSREALVIAEDTAFTAGQIGIMNVNGTTSI
DNAAVNNQFIDGELMKPADPKALDEIIAEAGKVDAAKYTSDSYNKMKTALDAALDADRYD
QDEIDKAVSDLREAINQLVLKPIDLTVLNSKIAEAKKLDPAKYTADSYARVKTALAAAEA
VNKKDQAAVDAAAKALSDAIAQLKTKQADLGALNQKIAEAKKLDAAKYTADSYAKVKTAL
AAAEAVNKNDQAAVNAAVKNLSDAIAQLRVKPVVPAVPKKNAVYTVNSLVYKVTKSSSKY
GTVKVMKAKKKSYTSISVPKTVKLNGYTFKVTEIYKNAFKNNTKLKTVKIADNVSKIGSY
AFAGCKKLKSVTIGKGLKSIGSKAFYNDKVLKSIVIKSTKVNKVYSKAFSGIHKNAYINV
PNKKASDYKKTFKNGGQAKTVKIK

Enzyme Prediction     

No EC number prediction in MGYG000000287_03540.

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GH2	387	889	2e-95	0.538563829787234
CBM66	1490	1635	3.4e-24	0.8903225806451613
CBM66	1654	1802	2.7e-21	0.9548387096774194

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
COG3250	LacZ	2.64e-39	413	842	15	429	Beta-galactosidase/beta-glucuronidase [Carbohydrate transport and metabolism].
PRK10150	PRK10150	9.54e-33	400	825	3	417	beta-D-glucuronidase; Provisional
pfam02837	Glyco_hydro_2_N	1.56e-25	413	568	4	163	Glycosyl hydrolases family 2, sugar binding domain. This family contains beta-galactosidase, beta-mannosidase and beta-glucuronidase activities and has a jelly-roll fold. The domain binds the sugar moiety during the sugar-hydrolysis reaction.
sd00036	LRR_3	7.75e-25	2070	2183	1	113	leucine-rich repeats. A leucine-rich repeat (LRR) is a structural protein motif of 20-30 amino acids that is unusually rich in the hydrophobic amino acid leucine. The conserved eleven-residue sequence motif (LxxLxLxxN/CxL) within the LRRs corresponds to the beta-strand and adjacent loop regions, whereas the remaining parts of the repeats are variable. LRRs fold together to form a solenoid protein domain, termed leucine-rich repeat domain. Leucine-rich repeats are usually involved in protein-protein interactions.
sd00036	LRR_3	3.16e-24	2051	2163	14	139	leucine-rich repeats. A leucine-rich repeat (LRR) is a structural protein motif of 20-30 amino acids that is unusually rich in the hydrophobic amino acid leucine. The conserved eleven-residue sequence motif (LxxLxLxxN/CxL) within the LRRs corresponds to the beta-strand and adjacent loop regions, whereas the remaining parts of the repeats are variable. LRRs fold together to form a solenoid protein domain, termed leucine-rich repeat domain. Leucine-rich repeats are usually involved in protein-protein interactions.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
AIQ74895.1	1.91e-131	397	1309	15	911
AWV34213.1	1.91e-131	397	1309	15	911
AWV99395.1	5.44e-127	399	1315	26	907
QGQ94319.1	1.49e-124	411	1309	29	907
AIQ24463.1	1.98e-124	397	1309	10	906

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
7SF2_A	1.58e-67	388	995	6	572	ChainA, Glycosyl hydrolase family 2, sugar binding domain protein [Bacteroides cellulosilyticus DSM 14838],7SF2_B Chain B, Glycosyl hydrolase family 2, sugar binding domain protein [Bacteroides cellulosilyticus DSM 14838],7SF2_C Chain C, Glycosyl hydrolase family 2, sugar binding domain protein [Bacteroides cellulosilyticus DSM 14838],7SF2_D Chain D, Glycosyl hydrolase family 2, sugar binding domain protein [Bacteroides cellulosilyticus DSM 14838],7SF2_E Chain E, Glycosyl hydrolase family 2, sugar binding domain protein [Bacteroides cellulosilyticus DSM 14838],7SF2_F Chain F, Glycosyl hydrolase family 2, sugar binding domain protein [Bacteroides cellulosilyticus DSM 14838]
5Z1A_A	1.03e-24	401	825	16	414	Thecrystal structure of Bacteroides fragilis beta-glucuronidase in complex with uronic isofagomine [Bacteroides fragilis NCTC 9343]
3CMG_A	1.70e-24	411	825	7	395	Crystalstructure of putative beta-galactosidase from Bacteroides fragilis [Bacteroides fragilis NCTC 9343]
5N6U_A	1.06e-23	411	833	27	457	Crystalstructure of Beta-D-Mannosidase from Dictyoglomus thermophilum. [Dictyoglomus thermophilum H-6-12],5N6U_B Crystal structure of Beta-D-Mannosidase from Dictyoglomus thermophilum. [Dictyoglomus thermophilum H-6-12],5N6U_C Crystal structure of Beta-D-Mannosidase from Dictyoglomus thermophilum. [Dictyoglomus thermophilum H-6-12],5N6U_D Crystal structure of Beta-D-Mannosidase from Dictyoglomus thermophilum. [Dictyoglomus thermophilum H-6-12]
5UJ6_A	1.48e-23	442	828	56	414	CrystalStructure of Bacteroides Uniformis beta-glucuronidase [Bacteroides uniformis str. 3978 T3 ii],5UJ6_B Crystal Structure of Bacteroides Uniformis beta-glucuronidase [Bacteroides uniformis str. 3978 T3 ii],6NZG_A Bacteroides uniformis beta-glucuronidase 2 covalently bound to cyclophellitol-6-carboxylate aziridine [Bacteroides uniformis],6NZG_B Bacteroides uniformis beta-glucuronidase 2 covalently bound to cyclophellitol-6-carboxylate aziridine [Bacteroides uniformis]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
T2KPJ7	3.11e-25	425	829	53	434	Putative beta-glucuronidase OS=Formosa agariphila (strain DSM 15362 / KCTC 12365 / LMG 23005 / KMM 3901 / M-2Alg 35-1) OX=1347342 GN=BN863_21970 PE=2 SV=1
P05804	1.98e-22	413	825	15	413	Beta-glucuronidase OS=Escherichia coli (strain K12) OX=83333 GN=uidA PE=1 SV=2
T2KM09	2.20e-20	397	826	41	433	Putative beta-glucuronidase OS=Formosa agariphila (strain DSM 15362 / KCTC 12365 / LMG 23005 / KMM 3901 / M-2Alg 35-1) OX=1347342 GN=BN863_22050 PE=2 SV=2
P77989	2.38e-19	456	817	49	379	Beta-galactosidase OS=Thermoanaerobacter pseudethanolicus (strain ATCC 33223 / 39E) OX=340099 GN=lacZ PE=3 SV=2
A7MN76	9.90e-17	399	826	51	472	Beta-galactosidase OS=Cronobacter sakazakii (strain ATCC BAA-894) OX=290339 GN=lacZ PE=3 SV=1

SignalP and Lipop Annotations

This protein is predicted as SP

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
0.000287	0.998918	0.000219	0.000205	0.000174	0.000150

TMHMM  Annotations     

There is no transmembrane helices in MGYG000000287_03540.