CAZyme Information: MGYG000000287_04321

Basic Information

• Species: Robinsoniella sp900539655
• Lineage: Bacteria; Firmicutes_A; Clostridia; Lachnospirales; Lachnospiraceae; Robinsoniella; Robinsoniella sp900539655
• CAZyme ID: MGYG000000287_04321
• CAZy Family: GH43
• CAZyme Description: hypothetical protein

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
3042		333768.62	4.9387

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000000287	7249439	MAG	Sweden	Europe

• Gene Location: Start: 14398; End: 23526 Strand: -

Full Sequence      

MKKRKIFSRLVAGAMAGIMAVTALPQLSMPDVKAPLQFRADQELIAHYPLETDALDVSGN
EYHGEVLGEGVTFSGDSLNLPGGKSDSTDYVALPQGIFDNQNQLTISMWIKNKDTKGNYA
AFFFGTKARGVLPVNYFLLNPCNPKGTYKSVFTDSVNESAPYNTEAGITNTVSTTEYMNQ
WAYYTVVLNTARENEGASGGKTVTAYLNGQNIGTQNLSRSVSDFGEGLVSLIGKSEYLED
PLFKGSFRDLRIYGKCLDDTQVNGLYETAFNRNELLEAKEGLSLLDTSKVIANLELPETV
GNDVSVTWESSNPAVIDNTGKVTMSDLEQNVTLTATLMRNGETVTKEFSVTVIAADGVLA
EAMSRVTVPYAITDNTILPTSLGDGITVKWECSDPSVIAADGTITAQDENMQLELKAAIA
KDDQLAYKEFVVLVMEKSASYVMGYTRNGNTELTDSMHLAYSTDGDKYTSLNYNTGILYP
KADLEENASNTIAGTTKRLLSPYLFRMADGKIGVAAVRNDAAGDKNAKGKILFYTTDDLM
EYEDCGLLPLHTELAVTEPRCEYDGVNEVYKITWKGSDGKLYCNTTTDFSEISTPEEITG
RENISVSSGITGAVLGNVLPITKAEAQGITGKLLPIENTGVEEQAYTFSTKAGQELAQSD
LDSVIKANYSDGTKKDIKVNWDAGSLKAINFNKEGTYQISGKAKLTRYPVLNGRADPDIY
FYNGKYYFIATGETANQSQINIREADIPLGVFSAADHELIPNVKKPRWAPELHEIDGKLY
IFLAVGDDWKQVQSSIMELKEGGNPLNKNDWKEAVRVRKQDGSYLYEYGITLDMTYFEVN
GISYVCWAQREMTTDRGFGTSDLYIATIDKENPYQLTSDPMCILRPQYGWDRFDATVDEG
PYVIQRDGTVYMTFSGSSVSNTYCVGLLTANEKSDLLDAASWRETGYPILQAESVSGEGG
PGHSAFTVDEDGNDLFVYHMKPGGGTRSASARRVHWAADGTPVLDMTVDRELKEEYRTVT
GTIIVEEGDVPEEERIVTADKEALVLNGVTENIVKGNLTLPAEGKNGSEITWFSDKEEVI
STASSANKDYAATPAGTVTRGSKDQKVTLRAVIRYGTFEDSKTFSVTVKAKETEKDYVGY
LYAYFSGDESRLDDQEIYFALSKDGITWSDLNENDPVLTSELGDKSVRDPYIIRSAEGDR
FYLIATDLDIRNSKYGNQWWTSRDGSKDLIIWESTDLVNWTNHRAVDVGSSIQADHVWAP
EAIYDEVTGEYLVYWSSSVKTDQYEKSRIYVAKTRDFYTFTEPEVYSDDPNGNIDASILK
VDQKYYRLIKDDREAYVTLESADRLLSYEQGEKNRGSVFKKVKNTELESYKGYYEGPTMF
RFINENKWCALVDEYGGAKRGYIPFVSENIGAQNSLHLMKDDTYLMPTGAKHGTVIPVTQ
EEYDALQEKWIVKSNMEQQEEPVLEYNFEEKSAGTMTDVSGNGNHGKTYGNAVFKKDTEK
GQVLYLNGSSGTYAEFPRGFFDGCDEMTLVMDVKPEITSNFFFTFGVGQDSSKYMFLRTK
PGNIRNAITTQGNGKERDVQINGSYQNKWSKVAIVMNGHSMSLYIDGVLADKRDYVRSIR
DLGSDLLAYLGKSFYSNDPYFKGSFDNIKVYNRVLSAKELGGDGPEPDISKDLLAHYKLE
ADGKEAGGRQKDAVLGGKGTHFTDGILRLDGGSKSDKNYVTLPEGLFDGQNSTTISMWIK
NKDTKMNTAAFSFSGKKKTGSYPADYFILNPCNLDGYYKAVFTDPAAGSTANPWTTEVGI
NNSSQNSISTVDYMNQWAYYTVTITKDRITGYLNGKKVGTSEIHNKTVADFGEKLTAYIG
ASEYADDTFAGDFCDLRIYSREFNLTEVSDLYQKSLPVIKVKEARASLKIGDGLSVIEDL
NLPAMAGECSVKWKSSKPAVVEENGTVHFPKEETKVTLTAVISSGAYAEEAVYELTIASE
ADAAAGRYRTALNIPKYITGNMQSTVNGETVIWSSEPKGVIDADGTVHRPAGDQENQQVV
VKAVIGNTVITKQAEVLAAGGQILSYVTEGGNLYTGEGDLLAASDSRRSDALFLAAKESQ
SGSFVPLNKGKAILYVKWDKDQKTNPDNQMGSPVLFRYKDGSLGAAASGNNKKDGIYIWD
SSENINFKNERYLVLNTDGKKVQDPDIVYDSLAECYKVFWTDEKGTSYVSMLNNLNAGAA
PVITIETDYEKTLVSAGIPENAVKEQASVFEADKEEYEALTRKYATVHNTGMESVVIRAN
AGDKVTLPETVTADYSDGSTKNLGVKWNQQDLDKIASGQAGTYEISGEVQQDSYAYPFIE
ERADPHIFYNEDDGYYYATGSYYEKNSNWNNENEARNNSYRKLDIRRARTIEGLKTADEH
YIMESKIGDRWGSFIWAPEFHKINGKWYCIVGAHDFGSAGIKSDTDWGWCSRSILIPYEG
SEEQMKNGGMLDAGQWGTPIEMNLPAPANASFDVSYYEDDGGQGYYIVPSAANLYVVKAK
GGEGVVPQPEGSPQLIKSGQWPWEYGVYEGSITANNPEGNDQLVVEGPYLFSYGDKVYIS
YSAATVDKFYTLGLMMADKGSDIMNPDSWTNINYPVLSSYDTVDGSIGGGEHVGGGHNSV
VLDEYGNLALVYHARPYPDPHEGKVGAGGLFDPCRHTVVKSINVAYDGTLIFNMTAEEEL
DPANKNIKAVVYISGKEHADKTALEVLIQNAKKTDTAKYTEASVKAMLDALSKAEEVKNR
ADASQSEVAQAAAALKNAMAKLEPRKDVPVPEKIAVQGVKLNRTSVTMKVKSKVTLTASI
SPSNAANKGVSFTSSNKSVASVNGNGTITAGRSGTAVITVVTADGGKKATCKITVKAPVI
KLNASSITLQVKKTTTTVKIKTSSPAGEKISAAKSGNKKLVSVKVKKGKMTITGKKKGRT
YITVTSTLGGKVKLKVKVQTGKVATKSIKLDKKLSIKKGKSLSLNVVRNPITATEKLEWT
SSNKKVAKVTSKGLVRAKKKGTTTITVKSSNGKSAKCKIKVE

Enzyme Prediction     

No EC number prediction in MGYG000000287_04321.

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GH43	714	1002	8.3e-92	0.9966555183946488
GH43	1185	1395	5.7e-57	0.9856459330143541
GH43	2336	2689	6.9e-51	0.9863945578231292

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
cd18818	GH43_GbtXyl43B-like	1.47e-146	715	981	1	273	Glycosyl hydrolase family 43 such as Geobacillus thermoleovorans IT-08 beta-xylosidase/exo-xylanase (GbtXyl43B). This glycosyl hydrolase family 43 (GH43) subgroup includes the characterized enzymes Geobacillus thermoleovorans IT-08 beta-xylosidase (EC 3.2.1.37) / exo-xylanase (GbtXyl43B), and Paenibacillus sp. strain E18 alpha-L-arabinofuranosidase (EC 3.2.1.55) Abf43B. It belongs to the glycosyl hydrolase clan F (according to carbohydrate-active enzymes database (CAZY)) which includes family 43 (GH43) and 62 (GH62) families. GH43 are inverting enzymes (i.e. they invert the stereochemistry of the anomeric carbon atom of the substrate) that have an aspartate as the catalytic general base, a glutamate as the catalytic general acid and another aspartate that is responsible for pKa modulation and orienting the catalytic acid. Many GH43 enzymes display both alpha-L-arabinofuranosidase and beta-D-xylosidase activity using aryl-glycosides as substrates. A common structural feature of GH43 enzymes is a 5-bladed beta-propeller domain that contains the catalytic acid and catalytic base. A long V-shaped groove, partially enclosed at one end, forms a single extended substrate-binding surface across the face of the propeller.
cd08983	GH43_Bt3655-like	2.02e-96	1170	1450	1	262	Glycosyl hydrolase family 43 protein such as Bacteroides thetaiotaomicron VPI-5482 arabinofuranosidase Bt3655. This glycosyl hydrolase family 43 (GH43)-like family includes the characterized arabinofuranosidases (EC 3.2.1.55): Bacteroides thetaiotaomicron VPI-5482 (Bt3655;BT_3655) and Penicillium chrysogenum 31B Abf43B, as well as Bifidobacterium adolescentis ATCC 15703 beta-xylosidase (EC 3.2.1.37) BAD_1527. It belongs to the glycosyl hydrolase clan F (according to carbohydrate-active enzymes database (CAZY)) which includes family 43 (GH43) and 62 (GH62) families. GH43 includes enzymes with beta-xylosidase (EC 3.2.1.37), beta-1,3-xylosidase (EC 3.2.1.-), alpha-L-arabinofuranosidase (EC 3.2.1.55), arabinanase (EC 3.2.1.99), xylanase (EC 3.2.1.8), endo-alpha-L-arabinanases (beta-xylanases) and galactan 1,3-beta-galactosidase (EC 3.2.1.145) activities. GH43 are inverting enzymes (i.e. they invert the stereochemistry of the anomeric carbon atom of the substrate) that have an aspartate as the catalytic general base, a glutamate as the catalytic general acid and another aspartate that is responsible for pKa modulation and orienting the catalytic acid. Many GH43 enzymes display both alpha-L-arabinofuranosidase and beta-D-xylosidase activity using aryl-glycosides as substrates. A common structural feature of GH43 enzymes is a 5-bladed beta-propeller domain that contains the catalytic acid and catalytic base. A long V-shaped groove, partially enclosed at one end, forms a single extended substrate-binding surface across the face of the propeller.
cd18819	GH43_LbAraf43-like	1.15e-72	715	979	1	275	Glycosyl hydrolase family 43 proteins similar to Lactobacillus brevis alpha-L-arabinofuranosidase LbAraf43 and Geobacillus thermoleovorans GbtXyl43B. This uncharacterized glycosyl hydrolase family 43 (GH43) subgroup belongs to a subgroup which includes enzymes with beta-xylosidase (EC 3.2.1.37), alpha-L-arabinofuranosidase (EC 3.2.1.55) and possibly bifunctional xylosidase/arabinofuranosidase activities, similar to Lactobacillus brevis alpha-L-arabinofuranosidase LbAraf43 and Geobacillus thermoleovorans IT-08 beta-xylosidase / exo-xylanase (GbtXyl43B). It belongs to the glycosyl hydrolase clan F (according to carbohydrate-active enzymes database (CAZY)) which includes family 43 (GH43) and 62 (GH62) families. GH43 are inverting enzymes (i.e. they invert the stereochemistry of the anomeric carbon atom of the substrate) that have an aspartate as the catalytic general base, a glutamate as the catalytic general acid and another aspartate that is responsible for pKa modulation and orienting the catalytic acid. Many GH43 enzymes display both alpha-L-arabinofuranosidase and beta-D-xylosidase activity using aryl-glycosides as substrates. A common structural feature of GH43 enzymes is a 5-bladed beta-propeller domain that contains the catalytic acid and catalytic base. A long V-shaped groove, partially enclosed at one end, forms a single extended substrate-binding surface across the face of the propeller.
cd18819	GH43_LbAraf43-like	1.72e-66	2343	2655	1	277	Glycosyl hydrolase family 43 proteins similar to Lactobacillus brevis alpha-L-arabinofuranosidase LbAraf43 and Geobacillus thermoleovorans GbtXyl43B. This uncharacterized glycosyl hydrolase family 43 (GH43) subgroup belongs to a subgroup which includes enzymes with beta-xylosidase (EC 3.2.1.37), alpha-L-arabinofuranosidase (EC 3.2.1.55) and possibly bifunctional xylosidase/arabinofuranosidase activities, similar to Lactobacillus brevis alpha-L-arabinofuranosidase LbAraf43 and Geobacillus thermoleovorans IT-08 beta-xylosidase / exo-xylanase (GbtXyl43B). It belongs to the glycosyl hydrolase clan F (according to carbohydrate-active enzymes database (CAZY)) which includes family 43 (GH43) and 62 (GH62) families. GH43 are inverting enzymes (i.e. they invert the stereochemistry of the anomeric carbon atom of the substrate) that have an aspartate as the catalytic general base, a glutamate as the catalytic general acid and another aspartate that is responsible for pKa modulation and orienting the catalytic acid. Many GH43 enzymes display both alpha-L-arabinofuranosidase and beta-D-xylosidase activity using aryl-glycosides as substrates. A common structural feature of GH43 enzymes is a 5-bladed beta-propeller domain that contains the catalytic acid and catalytic base. A long V-shaped groove, partially enclosed at one end, forms a single extended substrate-binding surface across the face of the propeller.
cd08980	GH43_LbAraf43-like	1.41e-59	715	979	1	274	Glycosyl hydrolase family 43 proteins such as Lactobacillus brevis alpha-L-arabinofuranosidase LbAraf43 and Geobacillus thermoleovorans GbtXyl43B. This glycosyl hydrolase family 43 (GH43) subgroup includes enzymes with beta-xylosidase (EC 3.2.1.37), alpha-L-arabinofuranosidase (EC 3.2.1.55) and possibly bifunctional xylosidase/arabinofuranosidase activities. In addition to Lactobacillus brevis alpha-L-arabinofuranosidase LbAraf43 and Geobacillus thermoleovorans IT-08 beta-xylosidase / exo-xylanase (GbtXyl43B). It belongs to the glycosyl hydrolase clan F (according to carbohydrate-active enzymes database (CAZY)) which includes family 43 (GH43) and 62 (GH62) familiesGH43 are inverting enzymes (i.e. they invert the stereochemistry of the anomeric carbon atom of the substrate) that have an aspartate as the catalytic general base, a glutamate as the catalytic general acid and another aspartate that is responsible for pKa modulation and orienting the catalytic acid. Many GH43 enzymes display both alpha-L-arabinofuranosidase and beta-D-xylosidase activity using aryl-glycosides as substrates. A common structural feature of GH43 enzymes is a 5-bladed beta-propeller domain that contains the catalytic acid and catalytic base. A long V-shaped groove, partially enclosed at one end, forms a single extended substrate-binding surface across the face of the propeller.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
QNF28727.1	6.69e-258	41	1068	37	1054
BBF42300.1	1.72e-165	19	1135	17	1157
AXF99322.1	2.39e-163	1039	2740	261	1869
QSG89643.1	4.45e-163	1039	2740	264	1872
VEG80427.1	4.45e-163	1039	2740	264	1872

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
3AKF_A	6.23e-45	709	1002	14	310	Crystalstructure of exo-1,5-alpha-L-arabinofuranosidase [Streptomyces avermitilis MA-4680 = NBRC 14893],3AKG_A Crystal structure of exo-1,5-alpha-L-arabinofuranosidase complexed with alpha-1,5-L-arabinofuranobiose [Streptomyces avermitilis MA-4680 = NBRC 14893],3AKH_A Crystal structure of exo-1,5-alpha-L-arabinofuranosidase complexed with alpha-1,5-L-arabinofuranotriose [Streptomyces avermitilis MA-4680 = NBRC 14893],3AKI_A Crystal structure of exo-1,5-alpha-L-arabinofuranosidase complexed with alpha-L-arabinofuranosyl azido [Streptomyces avermitilis MA-4680 = NBRC 14893]
3K1U_A	1.92e-43	709	1002	12	312	Beta-xylosidase,family 43 glycosyl hydrolase from Clostridium acetobutylicum [Clostridium acetobutylicum]
5M8E_A	2.68e-41	709	981	28	303	Crystalstructure of a GH43 arabonofuranosidase from Weissella sp. strain 142 [Weissella cibaria],5M8E_B Crystal structure of a GH43 arabonofuranosidase from Weissella sp. strain 142 [Weissella cibaria]
5M8B_A	4.19e-38	709	981	30	305	ChainA, Alpha-L-arabinofuranosidase II [Levilactobacillus brevis],5M8B_B Chain B, Alpha-L-arabinofuranosidase II [Levilactobacillus brevis]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
Q82P90	4.46e-44	709	1002	40	336	Extracellular exo-alpha-(1->5)-L-arabinofuranosidase OS=Streptomyces avermitilis (strain ATCC 31267 / DSM 46492 / JCM 5070 / NBRC 14893 / NCIMB 12804 / NRRL 8165 / MA-4680) OX=227882 GN=Araf43A PE=1 SV=1
G4MMH2	1.34e-42	714	1002	49	342	Alpha-L-arabinofuranosidase B OS=Magnaporthe oryzae (strain 70-15 / ATCC MYA-4617 / FGSC 8958) OX=242507 GN=abfB PE=1 SV=1
P82594	8.28e-40	683	981	31	319	Extracellular exo-alpha-(1->5)-L-arabinofuranosidase OS=Streptomyces chartreusis OX=1969 PE=1 SV=1
A0A3R0A696	1.88e-30	938	1333	255	680	Alpha-L-arabinofuranosidase OS=Bifidobacterium longum subsp. longum (strain ATCC 15707 / DSM 20219 / JCM 1217 / NCTC 11818 / E194b) OX=565042 GN=blArafA PE=1 SV=1
P85991	5.31e-06	2794	2875	175	257	Ig-like virion protein OS=Serratia phage KSP90 OX=552528 PE=1 SV=2

SignalP and Lipop Annotations

This protein is predicted as SP

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
0.110106	0.887046	0.001476	0.000676	0.000345	0.000333

TMHMM  Annotations     

start	end
7	29