CAZyme Information: MGYG000000289_00182

Basic Information

• Species: Prevotella buccae
• Lineage: Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Bacteroidaceae; Prevotella; Prevotella buccae
• CAZyme ID: MGYG000000289_00182
• CAZy Family: GH115
• CAZyme Description: hypothetical protein

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
763	MGYG000000289_1|CGC4	86514.59	7.6091

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000000289	3001000	MAG	Sweden	Europe

• Gene Location: Start: 192039; End: 194330 Strand: +

Full Sequence      

MKKIVWSIVVALLTVRAGAFELKSGQTVTVSCDTGRTEAVVKSALRMLTGDMAKVLSAKV
CIGKEGSIIVRQDGRALNNRKEAFRLQVSGDTLRITGSDAHGVAYGLLEVSRLLGVSPWE
WWADATPAKLSAFKLPDGYVNEQAPDVEFRGIFINDEDWGLMPWASQTYEPGAKGRIGPK
ANARIFELLLRLRANTYWPAMHECTQPFFLTEGNRETAKEYGIYIGGSHCEPMASSTAGE
WPRRGVGEYNYVTNRDNVCHFWESRVREVAGQPTLYTIGMRGLHDGAMNGAKTIGEQKTV
LERVFVDQRQLLGRYVNRDVTKVPQVFIPYKEVLDVYNAGLQVPEDVCLMWCDDNYGYIR
HFPTEAERWRKGGNGIYYHVSYWGRPHDYLWLGTFSPALLYQQMATAYDRGIRKIWILNV
GDIKPCEYQTELFMDMAWSMEAVRRQGPGAHLGSFLRREFGDKVGAQLLPVMWEHYRLAY
IRKPEFMGGTRTEEPDRTYWNTVRDLPWSNDYITRRLSDYQTIENAAEALAGEIREDARD
EYFQLVKYPVLAAAEMNKKMLYAQQARHGLCGWEKSDAAHDSIVRLTQTYNALRDGKWQR
MMDCQPRRLPVFNPVAHTRAEAPMPQEYAYLYRWNGSECAGGDFTLCEGLGYEGKAVQVG
KGKSVLFSFHATGIDSVSVEVSLLPVHPLTGKSIRFRLSLDGTESPVTDYATQGRSEEWK
TNVLCNRAIRRFILPLKRQAAHQLILTALDDGVVIDEVMLGDR

Enzyme Prediction     

EC	3.2.1.-

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GH115	19	758	3.6e-216	0.9956958393113343

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
pfam15979	Glyco_hydro_115	1.71e-177	143	486	1	334	Glycosyl hydrolase family 115. Glyco_hydro_115 is a family of glycoside hydrolases likely to have the activity of xylan a-1,2-glucuronidase, EC:3.2.1.131, or a-(4-O-methyl)-glucuronidase EC:3.2.1.-.
pfam17829	GH115_C	1.49e-08	648	756	31	154	Gylcosyl hydrolase family 115 C-terminal domain. This domain is found at the C-terminus of glycosyl hydrolase family 115 proteins. This domain has a beta-sandwich fold.
pfam02838	Glyco_hydro_20b	2.48e-05	13	107	11	101	Glycosyl hydrolase family 20, domain 2. This domain has a zincin-like fold.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
QJR62690.1	0.0	1	760	1	763
QJR71288.1	0.0	1	760	1	763
QJR66948.1	0.0	1	760	1	763
QJR75325.1	0.0	1	760	1	763
AII67826.1	0.0	1	760	1	763

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
5BY3_A	0.0	20	760	20	773	Anovel family GH115 4-O-Methyl-alpha-glucuronidase, BtGH115A, with specificity for decorated arabinogalactans [Bacteroides thetaiotaomicron VPI-5482]
4C90_A	5.66e-90	57	602	91	640	Evidencethat GH115 alpha-glucuronidase activity is dependent on conformational flexibility [Bacteroides ovatus],4C90_B Evidence that GH115 alpha-glucuronidase activity is dependent on conformational flexibility [Bacteroides ovatus],4C91_A Evidence that GH115 alpha-glucuronidase activity is dependent on conformational flexibility [Bacteroides ovatus],4C91_B Evidence that GH115 alpha-glucuronidase activity is dependent on conformational flexibility [Bacteroides ovatus]
4ZMH_A	2.76e-83	62	598	73	606	Crystalstructure of a five-domain GH115 alpha-Glucuronidase from the Marine Bacterium Saccharophagus degradans 2-40T [Saccharophagus degradans 2-40],4ZMH_B Crystal structure of a five-domain GH115 alpha-Glucuronidase from the Marine Bacterium Saccharophagus degradans 2-40T [Saccharophagus degradans 2-40]
7PUG_A	5.42e-82	91	602	114	625	ChainA, xylan alpha-1,2-glucuronidase [uncultured bacterium]
7PXQ_A	1.02e-81	91	602	113	624	ChainA, xylan alpha-1,2-glucuronidase [uncultured bacterium]

Swiss-Prot Hits     

has no Swissprot hit.

SignalP and Lipop Annotations

This protein is predicted as SP

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
0.000271	0.999046	0.000172	0.000160	0.000164	0.000153

TMHMM  Annotations     

There is no transmembrane helices in MGYG000000289_00182.