CAZyme Information: MGYG000000289_01163

Basic Information

• Species: Prevotella buccae
• Lineage: Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Bacteroidaceae; Prevotella; Prevotella buccae
• CAZyme ID: MGYG000000289_01163
• CAZy Family: GH127
• CAZyme Description: Non-reducing end beta-L-arabinofuranosidase

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
678	MGYG000000289_13|CGC1	75502.74	8.4363

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000000289	3001000	MAG	Sweden	Europe

• Gene Location: Start: 40209; End: 42245 Strand: +

Full Sequence      

MRKIISTVVVAAVMTLPAMAQKTSDYGYPFTQVPFTSVRIAPNTFWGDRLKAAREVTVPL
AFSKCKSEGRYDNFVKAARPDEKYDVSKFMGFSFDDTDVYKTIEGASYILQTYPDKRLKA
YIDSVLDVVGAAQEPDGYLYTARTINPKKPHKWSGSKRWEKVEVLSHELYNLGHMVDAAC
AHYQATGSTKFLDIAKRYADCVIREVGPGQGQATVVPGHQIAEMALARLYTVTGDKRYLD
EAKYFLDYRGKTHIRDLYSQSDKPVTEQREAWGHAVRAGYMYAGMADVAALTKDSAYIKA
IDRIWNNIVGRKYYLTGGVGARHSGEAFGADYELPNMTAYNETCAAIAQVYLNERMFLLH
GESKYIDCLERTLYNGVISGMSMDGGRFFYPNPLSSDGKYKFNADGTTTRQPWFGCACCP
SNLSRFIPSLPGYLYGVKGNDIYVNLFAGNTSTISVNGKEVVLEETTQYPWEGDISIKVA
KSSAKVANLLIRIPGWVRNRVVPSDLYRYSDDEKPAYSVTVNGKRVEDDPDANKGYLPVR
GIRKGDVIRIHFDMPVRTVVASDRVADDRGKVAVERGPLVYCAESADNGGAAVLHAVVNK
KPAFSVVNNYSIDNTEAHAAPFTVKAIKTPAQILRESADGGMEVEKLDLTLIPYYAWNHR
GANQMNVWFVQNLSLLDK

Enzyme Prediction     

No EC number prediction in MGYG000000289_01163.

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GH127	43	582	6.2e-199	0.9904580152671756

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
pfam07944	Glyco_hydro_127	0.0	37	582	1	503	Beta-L-arabinofuranosidase, GH127. One member of this family, from Bidobacterium longicum, UniProtKB:E8MGH8, has been characterized as an unusual beta-L-arabinofuranosidase enzyme, EC:3.2.1.185. It rleases l-arabinose from the l-arabinofuranose (Araf)-beta1,2-Araf disaccharide and also transglycosylates 1-alkanols with retention of the anomeric configuration. Terminal beta-l-arabinofuranosyl residues have been found in arabinogalactan proteins from a mumber of different plantt species. beta-l-Arabinofuranosyl linkages with 1-4 arabinofuranosides are also found in the sugar chains of extensin and solanaceous lectins, hydroxyproline (Hyp)2-rich glycoproteins that are widely observed in plant cell wall fractions. The critical residue for catalytic activity is Glu-338, in a ET/SCAS sequence context.
COG3533	COG3533	1.26e-153	30	668	9	584	Uncharacterized conserved protein, DUF1680 family [Function unknown].
cd04791	LanC_SerThrkinase	5.74e-05	183	318	99	243	Lanthionine synthetase C-like domain associated with serine/threonine kinases. Some members of this subgroup lack the zinc binding site and the active site residues, and therefore are most likely inactive. The function of this domain is unknown.
COG1331	YyaL	0.001	225	373	419	564	Uncharacterized conserved protein YyaL, SSP411 family, contains thoiredoxin and six-hairpin glycosidase-like domains [General function prediction only].
COG1331	YyaL	0.002	173	304	414	563	Uncharacterized conserved protein YyaL, SSP411 family, contains thoiredoxin and six-hairpin glycosidase-like domains [General function prediction only].

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
QNT67047.1	0.0	1	678	1	691
BCS84603.1	0.0	27	674	13	659
VEH14288.1	0.0	1	668	1	666
AVM58298.1	2.31e-312	18	668	19	654
AVM52620.1	8.23e-310	3	668	2	654

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
6EX6_A	1.92e-286	25	674	2	628	TheGH127, Beta-arabinofuranosidase, BT3674 [Bacteroides thetaiotaomicron VPI-5482],6EX6_B The GH127, Beta-arabinofuranosidase, BT3674 [Bacteroides thetaiotaomicron VPI-5482]
4QJY_A	8.38e-132	45	668	22	644	Crystalstructure of native Ara127N, a GH127 beta-L-arabinofuranosidase from Geobacillus Stearothermophilus T6 [Geobacillus stearothermophilus],4QJY_B Crystal structure of native Ara127N, a GH127 beta-L-arabinofuranosidase from Geobacillus Stearothermophilus T6 [Geobacillus stearothermophilus]
4QK0_A	1.92e-126	45	668	22	644	Crystalstructure of Ara127N-Se, a GH127 beta-L-arabinofuranosidase from Geobacillus Stearothermophilus T6 [Geobacillus stearothermophilus],4QK0_B Crystal structure of Ara127N-Se, a GH127 beta-L-arabinofuranosidase from Geobacillus Stearothermophilus T6 [Geobacillus stearothermophilus],4QK0_C Crystal structure of Ara127N-Se, a GH127 beta-L-arabinofuranosidase from Geobacillus Stearothermophilus T6 [Geobacillus stearothermophilus],4QK0_D Crystal structure of Ara127N-Se, a GH127 beta-L-arabinofuranosidase from Geobacillus Stearothermophilus T6 [Geobacillus stearothermophilus]
3WRE_A	2.03e-113	88	668	67	655	Thecrystal structure of native HypBA1 from Bifidobacterium longum JCM 1217 [Bifidobacterium longum subsp. longum JCM 1217],3WRG_A The complex structure of HypBA1 with L-arabinose [Bifidobacterium longum subsp. longum JCM 1217]
3WKW_A	2.72e-113	88	668	67	655	Crystalstructure of GH127 beta-L-arabinofuranosidase HypBA1 from Bifidobacterium longum ligand free form [Bifidobacterium longum subsp. longum JCM 1217],3WKX_A Crystal structure of GH127 beta-L-arabinofuranosidase HypBA1 from Bifidobacterium longum arabinose complex form [Bifidobacterium longum subsp. longum JCM 1217],7BZL_A Chain A, Non-reducing end beta-L-arabinofuranosidase [Bifidobacterium longum subsp. longum JCM 1217],7DIF_A Chain A, Non-reducing end beta-L-arabinofuranosidase [Bifidobacterium longum subsp. longum JCM 1217],7EXV_A Chain A, Non-reducing end beta-L-arabinofuranosidase [Bifidobacterium longum subsp. longum JCM 1217],7EXW_A Chain A, Non-reducing end beta-L-arabinofuranosidase [Bifidobacterium longum subsp. longum JCM 1217]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
E8MGH8	1.11e-112	88	668	67	655	Non-reducing end beta-L-arabinofuranosidase OS=Bifidobacterium longum subsp. longum (strain ATCC 15707 / DSM 20219 / JCM 1217 / NCTC 11818 / E194b) OX=565042 GN=hypBA1 PE=1 SV=1

SignalP and Lipop Annotations

This protein is predicted as SP

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
0.000262	0.999060	0.000173	0.000173	0.000156	0.000140

TMHMM  Annotations     

There is no transmembrane helices in MGYG000000289_01163.