CAZyme Information: MGYG000000294_01060

Basic Information

• Species: Phascolarctobacterium_A sp900544885
• Lineage: Bacteria; Firmicutes_C; Negativicutes; Acidaminococcales; Acidaminococcaceae; Phascolarctobacterium_A; Phascolarctobacterium_A sp900544885
• CAZyme ID: MGYG000000294_01060
• CAZy Family: GT51
• CAZyme Description: Penicillin-binding protein 1F

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
687		75456.16	7.8721

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000000294	1781860	MAG	Sweden	Europe

• Gene Location: Start: 6969; End: 9032 Strand: +

Full Sequence      

MLKILKTIIICLLSLGIMGVSILYGFSSFFAPPNMDETLIPDAASQFYDINGNAIYTTLS
EERRIPVTINDIPKHVQQAFIAIEDNRFYEHGGIDYRGTARALVSTLSGSEVQGGSTITQ
QLAKNAFLTQERSIIRKIKEAFIAKELEHKYTKDEILTMYLNQIYFGQGAYGIESASMYY
FGKHVKQLDVAEAATLAAIPKSPNYFNPFENPQESKKRQELVIDQMVKYGLISAEDGAAA
KAEKLSFNTYHKKSTDPRSYFFDMITQKVIEEVGADALYKGGLKIYTTLDSDMQRAAERA
MKHLPTYYTDGKKITQPQMALVAIDPTTGYVKAMIGGRGDDKFNRATLAVRQPGSAFKPF
VYLTAMQNGYTPASVIEDKVEEFSPGWKPQNSDRKEHGKVSLRSALIRSLNVPTVKLAQE
VGVDKIIANAEKMGISTMVESGAYSDANLAMALGGLSKGVNPLEMAAAYGVLATNGMYCK
PIALLKIVDREGKVLYEAKKSEAKRVIDAEAAYLTTNMMQDVFVSGTASGMGIGRQAAGK
TGTTDTYIDAWFVGYTPNLSTAVWVGDDNNKPMNYMYGSGAPLSIWHDFMVNALASAPAA
SFVNPGVTVPPEPVIKQDPKDGKDKKKDKDKDKNKGKDSDVIEKEEDETQEPSVDSEPVK
PARRSMKDRVNQVLGKPVVSNQPTPAK

Enzyme Prediction     

No EC number prediction in MGYG000000294_01060.

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GT51	54	227	7.5e-76	0.9774011299435028

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
COG0744	MrcB	0.0	3	613	9	616	Membrane carboxypeptidase (penicillin-binding protein) [Cell wall/membrane/envelope biogenesis].
COG5009	MrcA	0.0	1	605	1	730	Membrane carboxypeptidase/penicillin-binding protein [Cell wall/membrane/envelope biogenesis].
TIGR02074	PBP_1a_fam	0.0	63	595	1	530	penicillin-binding protein, 1A family. Bacterial that synthesize a cell wall of peptidoglycan (murein) generally have several transglycosylases and transpeptidases for the task. This family consists of bifunctional transglycosylase/transpeptidase penicillin-binding proteins (PBP). In the Proteobacteria, this family includes PBP 1A but not the paralogous PBP 1B (TIGR02071). This family also includes related proteins, often designated PBP 1A, from other bacterial lineages. [Cell envelope, Biosynthesis and degradation of murein sacculus and peptidoglycan]
TIGR02071	PBP_1b	1.19e-140	60	614	143	695	penicillin-binding protein 1B. Bacterial that synthesize a cell wall of peptidoglycan (murein) generally have several transglycosylases and transpeptidases for the task. This family consists of a particular bifunctional transglycosylase/transpeptidase in E. coli and other Proteobacteria, designated penicillin-binding protein 1B. [Cell envelope, Biosynthesis and degradation of murein sacculus and peptidoglycan]
TIGR02073	PBP_1c	2.23e-112	48	611	15	559	penicillin-binding protein 1C. This subfamily of the penicillin binding proteins includes the member from E. coli designated penicillin-binding protein 1C. Members have both transglycosylase and transpeptidase domains and are involved in forming cross-links in the late stages of peptidoglycan biosynthesis. All members of this subfamily are presumed to have the same basic function. [Cell envelope, Biosynthesis and degradation of murein sacculus and peptidoglycan]

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
QTV77229.1	0.0	1	685	1	681
QNP76735.1	7.71e-310	3	620	3	620
BBG64167.1	7.71e-310	3	620	3	620
AEQ22139.1	8.11e-222	9	623	32	640
QJW47990.1	4.08e-221	8	616	25	642

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
4OON_A	2.03e-91	48	605	24	734	Crystalstructure of PBP1a in complex with compound 17 ((4Z,8S,11E,14S)-5-(2-amino-1,3-thiazol-4-yl)-14-(5,6-dihydroxy-1,3-dioxo-1,3-dihydro-2H-isoindol-2-yl)-8-formyl-2-methyl-6-oxo-3,10-dioxa-4,7,11-triazatetradeca-4,11-diene-2,12,14-tricarboxylic acid) [Pseudomonas aeruginosa PAO1]
3UDF_A	2.23e-87	65	600	40	722	ChainA, Penicillin-binding protein 1a [Acinetobacter baumannii],3UDF_B Chain B, Penicillin-binding protein 1a [Acinetobacter baumannii],3UDI_A Chain A, Penicillin-binding protein 1a [Acinetobacter baumannii],3UDI_B Chain B, Penicillin-binding protein 1a [Acinetobacter baumannii],3UDX_A Chain A, Penicillin-binding protein 1a [Acinetobacter baumannii],3UDX_B Chain B, Penicillin-binding protein 1a [Acinetobacter baumannii],3UE0_A Chain A, Penicillin-binding protein 1a [Acinetobacter baumannii],3UE0_B Chain B, Penicillin-binding protein 1a [Acinetobacter baumannii],3UE1_A Chain A, Penicillin-binding protein 1a [Acinetobacter baumannii],3UE1_B Chain B, Penicillin-binding protein 1a [Acinetobacter baumannii]
3ZG8_B	5.08e-85	145	602	1	469	CrystalStructure of Penicillin Binding Protein 4 from Listeria monocytogenes in the Ampicillin bound form [Listeria monocytogenes],3ZG9_B Crystal Structure of Penicillin-Binding Protein 4 from Listeria monocytogenes in the Cefuroxime bound form [Listeria monocytogenes],3ZGA_B Crystal Structure of Penicillin-Binding Protein 4 from Listeria monocytogenes in the Carbenicillin bound form [Listeria monocytogenes]
5FGZ_A	6.28e-85	62	592	154	692	E.coli PBP1b in complex with FPI-1465 [Escherichia coli K-12],5HL9_A E. coli PBP1b in complex with acyl-ampicillin and moenomycin [Escherichia coli K-12],5HLA_A E. coli PBP1b in complex with acyl-cephalexin and moenomycin [Escherichia coli K-12],5HLB_A E. coli PBP1b in complex with acyl-aztreonam and moenomycin [Escherichia coli K-12],5HLD_A E. coli PBP1b in complex with acyl-CENTA and moenomycin [Escherichia coli K-12],6YN0_A Structure of E. coli PBP1b with a FtsN peptide activating transglycosylase activity [Escherichia coli K-12],7LQ6_A Chain A, Penicillin-binding protein 1B [Escherichia coli K-12]
3VMA_A	9.57e-85	62	592	175	713	CrystalStructure of the Full-Length Transglycosylase PBP1b from Escherichia coli [Escherichia coli K-12]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
P38050	6.67e-120	36	593	40	597	Penicillin-binding protein 1F OS=Bacillus subtilis (strain 168) OX=224308 GN=pbpF PE=2 SV=2
O66874	9.93e-119	13	621	4	691	Penicillin-binding protein 1A OS=Aquifex aeolicus (strain VF5) OX=224324 GN=mrcA PE=1 SV=1
P70997	4.29e-104	48	612	58	630	Penicillin-binding protein 2D OS=Bacillus subtilis (strain 168) OX=224308 GN=pbpG PE=2 SV=3
O87579	2.41e-99	10	605	16	717	Penicillin-binding protein 1A OS=Neisseria lactamica OX=486 GN=mrcA PE=3 SV=1
P0A0Z6	2.61e-97	11	594	17	706	Penicillin-binding protein 1A OS=Neisseria meningitidis serogroup B (strain MC58) OX=122586 GN=mrcA PE=3 SV=1

SignalP and Lipop Annotations

This protein is predicted as OTHER

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
0.905011	0.090306	0.001393	0.000613	0.000291	0.002415

TMHMM  Annotations     

start	end
7	29