CAZyme Information: MGYG000000295_01080

Basic Information

• Species: Clostridium_E sporosphaeroides
• Lineage: Bacteria; Firmicutes_A; Clostridia; Oscillospirales; Acutalibacteraceae; Clostridium_E; Clostridium_E sporosphaeroides
• CAZyme ID: MGYG000000295_01080
• CAZy Family: GT2
• CAZyme Description: hypothetical protein

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
775		89221.85	7.3991

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000000295	2812533	MAG	Sweden	Europe

• Gene Location: Start: 4437; End: 6764 Strand: -

Full Sequence      

MEKNLHGLGERMVRAGLINKEQLADALLWQEQENTLLGETLVNHGIITGEQLFRFIFTSP
GATVGEVLIGHGYIDREQLAEALRYQQKYGGRLGAICVSLGFLTAQQLDSALAQRVAPKG
RLGDELLRLGIITQEQLDTALETQRRSGGRLGEILLFLEYVTEEQLYRALATQGEIGRLG
SDVRLSDVKALPYGLANRYAAIVIREEKQYTVVAVESKLSEAAVKDIEAYLEKPIEQVLM
TNAERLRFWEQAYRRDEILGSVFALYDEQPENSAIETFTTPQLVTLLVLLAVFVVSLAAN
WFVTLLILVIIFQIIYLAMAIAKFWMVIKGARHNAQLRFSPEEVAAVDETKLPVYTLLIP
VYKEKEVLPHLLKRIQNLDYPKYKLDVRILLEENDPETIELVRAMRLPSYFTPIVVPASM
PQTKPKACNYGLLQAKGEYVVIYDAEDRPERDQLKKAYLAFQKLPEEYVCIQAKLNYFNS
RQNLLTKLFTQEYSMWFELLLVGIMQMDIPIPLGGTSNHFKMDFLRQVGGWDPFNVTEDA
DLGIRLYKHRYKTAILDSRTWEEANSNVRNWVRQRSRWIKGYMQTFFVHMREPAKFYRQV
GFRGMLGYLAMILGTPLLPLLNPIFWGMMVVWLIFHPFWVQMFFPGPIYYISVIQLILGN
FIFMYMNIVGTYYVIRDCAIKKDQPFSYGLIRSGLLTPLYWILMSVAAYKALIQLIVKPH
YWEKTHHGLTEHSDVAHLQYIEQLLTEEQVKNRKDKSVPSNSLESKGTLHETKSH

Enzyme Prediction     

No EC number prediction in MGYG000000295_01080.

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GT2	439	636	1.9e-23	0.949238578680203

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
cd06427	CESA_like_2	1.89e-150	353	593	1	241	CESA_like_2 is a member of the cellulose synthase superfamily. The cellulose synthase (CESA) superfamily includes a wide variety of glycosyltransferase family 2 enzymes that share the common characteristic of catalyzing the elongation of polysaccharide chains. The members include cellulose synthase catalytic subunit, chitin synthase, Glucan Biosynthesis protein and other families of CESA-like proteins. Cellulose synthase catalyzes the polymerization reaction of cellulose, an aggregate of unbranched polymers of beta-1,4-linked glucose residues in plants, most algae, some bacteria and fungi, and even some animals. In bacteria, algae and lower eukaryotes, there is a second unrelated type of cellulose synthase (Type II), which produces acylated cellulose, a derivative of cellulose. Chitin synthase catalyzes the incorporation of GlcNAc from substrate UDP-GlcNAc into chitin, which is a linear homopolymer of beta-(1,4)-linked GlcNAc residues and Glucan Biosynthesis protein catalyzes the elongation of beta-1,2 polyglucose chains of glucan.
COG1215	BcsA	6.51e-54	302	704	3	395	Glycosyltransferase, catalytic subunit of cellulose synthase and poly-beta-1,6-N-acetylglucosamine synthase [Cell motility].
cd06423	CESA_like	5.71e-37	357	539	1	180	CESA_like is the cellulose synthase superfamily. The cellulose synthase (CESA) superfamily includes a wide variety of glycosyltransferase family 2 enzymes that share the common characteristic of catalyzing the elongation of polysaccharide chains. The members include cellulose synthase catalytic subunit, chitin synthase, glucan biosynthesis protein and other families of CESA-like proteins. Cellulose synthase catalyzes the polymerization reaction of cellulose, an aggregate of unbranched polymers of beta-1,4-linked glucose residues in plants, most algae, some bacteria and fungi, and even some animals. In bacteria, algae and lower eukaryotes, there is a second unrelated type of cellulose synthase (Type II), which produces acylated cellulose, a derivative of cellulose. Chitin synthase catalyzes the incorporation of GlcNAc from substrate UDP-GlcNAc into chitin, which is a linear homopolymer of beta-(1,4)-linked GlcNAc residues and Glucan Biosynthesis protein catalyzes the elongation of beta-1,2 polyglucose chains of Glucan.
cd06437	CESA_CaSu_A2	1.94e-20	353	581	1	231	Cellulose synthase catalytic subunit A2 (CESA2) is a catalytic subunit or a catalytic subunit substitute of the cellulose synthase complex. Cellulose synthase (CESA) catalyzes the polymerization reaction of cellulose using UDP-glucose as the substrate. Cellulose is an aggregate of unbranched polymers of beta-1,4-linked glucose residues, which is an abundant polysaccharide produced by plants and in varying degrees by several other organisms including algae, bacteria, fungi, and even some animals. Genomes from higher plants harbor multiple CESA genes. There are ten in Arabidopsis. At least three different CESA proteins are required to form a functional complex. In Arabidopsis, CESA1, 3 and 6 and CESA4, 7 and 8, are required for cellulose biosynthesis during primary and secondary cell wall formation. CESA2 is very closely related to CESA6 and is viewed as a prime substitute for CESA6. They functionally compensate each other. The cesa2 and cesa6 double mutant plants were significantly smaller, while the single mutant plants were almost normal.
cd06421	CESA_CelA_like	2.52e-20	353	586	1	234	CESA_CelA_like are involved in the elongation of the glucan chain of cellulose. Family of proteins related to Agrobacterium tumefaciens CelA and Gluconacetobacter xylinus BscA. These proteins are involved in the elongation of the glucan chain of cellulose, an aggregate of unbranched polymers of beta-1,4-linked glucose residues. They are putative catalytic subunit of cellulose synthase, which is a glycosyltransferase using UDP-glucose as the substrate. The catalytic subunit is an integral membrane protein with 6 transmembrane segments and it is postulated that the protein is anchored in the membrane at the N-terminal end.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
QAT49194.1	5.21e-257	1	734	1	738
QNK42121.1	9.37e-256	8	731	8	735
AKA67429.1	3.37e-253	4	731	7	734
AWI06117.1	1.10e-251	4	731	7	734
AZV55411.1	9.16e-251	1	730	1	730

PDB Hits     

has no PDB hit.

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
P96587	3.58e-20	351	598	47	293	Uncharacterized glycosyltransferase YdaM OS=Bacillus subtilis (strain 168) OX=224308 GN=ydaM PE=3 SV=1
Q9ZQB9	9.87e-16	352	619	240	514	Probable xyloglucan glycosyltransferase 12 OS=Arabidopsis thaliana OX=3702 GN=CSLC12 PE=1 SV=1
Q9SB75	4.68e-14	317	586	191	467	Probable xyloglucan glycosyltransferase 5 OS=Arabidopsis thaliana OX=3702 GN=CSLC5 PE=1 SV=1
Q9SJA2	6.16e-14	350	592	224	473	Probable xyloglucan glycosyltransferase 8 OS=Arabidopsis thaliana OX=3702 GN=CSLC8 PE=2 SV=1
Q7PC69	4.51e-13	317	586	222	501	Probable xyloglucan glycosyltransferase 3 OS=Oryza sativa subsp. japonica OX=39947 GN=CSLC3 PE=2 SV=1

SignalP and Lipop Annotations

This protein is predicted as OTHER

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
1.000057	0.000001	0.000000	0.000000	0.000000	0.000000

TMHMM  Annotations     

start	end
283	302
306	328
606	628
643	665
686	708