CAZyme Information: MGYG000000297_00885

Basic Information

• Species: UBA7057 sp900543675
• Lineage: Bacteria; Firmicutes; Bacilli; RF39; UBA660; UBA7057; UBA7057 sp900543675
• CAZyme ID: MGYG000000297_00885
• CAZy Family: CBM50
• CAZyme Description: Cell division suppressor protein YneA

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
464		51221.36	4.7137

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000000297	1541132	MAG	Sweden	Europe

• Gene Location: Start: 19519; End: 20913 Strand: -

Full Sequence      

MNEQRVTGVVVDAGHGGDDPGAVSGGLREKDFTLEAANYMYNRFKELGVPVAITRDSDVT
LSRQQRLNTMNNSFGTDPKVIILSNHINSGGGEGAEIIYPLRSSSTLPRQILEEIGSEGQ
PTRTYYQKRLPEDPSKDYYYIMRETPDTTSLLIEYGFIDNPNDVKRLQNNLLDYVEAVVR
AVTRYIGVPYVAPDGISEETYVVQKGDSLYSIAQKFGTTVDQLKKWNNLTSNILQIGQVL
IVSEKEETAPPSENYINYTVKSGDTLYKIANEFNTSVQQIIDLNDLGTTVLSIGQTLRIP
TSGSSTTNTYTVKSGDSLWKIANQFGVSVDDLINANNLTSTVLQVGQQLIIPNQGSSTPP
IGENTPISYTVKSGDSLWSIANRYNTTVAELRRLNNLTSDVLQIGQVLRIPNSSMPNPIT
YTVKSGDSLWSIANRYNTTVAELRRLNNLTSDVLQIGQQLIIPQ

Enzyme Prediction     

No EC number prediction in MGYG000000297_00885.

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
CBM50	369	411	3.2e-18	0.975
CBM50	421	463	6.5e-18	0.975
CBM50	201	242	1.6e-17	0.95
CBM50	310	352	1.7e-17	0.975
CBM50	258	300	5.1e-16	0.975

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
PRK06347	PRK06347	7.92e-49	197	462	329	591	1,4-beta-N-acetylmuramoylhydrolase.
PRK06347	PRK06347	4.95e-37	301	460	324	521	1,4-beta-N-acetylmuramoylhydrolase.
cd02696	MurNAc-LAA	2.44e-34	9	184	2	169	N-acetylmuramoyl-L-alanine amidase or MurNAc-LAA (also known as peptidoglycan aminohydrolase, NAMLA amidase, NAMLAA, Amidase 3, and peptidoglycan amidase; EC 3.5.1.28) is an autolysin that hydrolyzes the amide bond between N-acetylmuramoyl and L-amino acids in certain cell wall glycopeptides. These proteins are Zn-dependent peptidases with highly conserved residues involved in cation co-ordination. MurNAc-LAA in this family is one of several peptidoglycan hydrolases (PGHs) found in bacterial and bacteriophage or prophage genomes that are involved in the degradation of the peptidoglycan. In Escherichia coli, there are five MurNAc-LAAs present: AmiA, AmiB, AmiC and AmiD that are periplasmic, and AmpD that is cytoplasmic. Three of these (AmiA, AmiB and AmiC) belong to this family, the other two (AmiD and AmpD) do not. E. coli AmiA, AmiB and AmiC play an important role in cleaving the septum to release daughter cells after cell division. In general, bacterial MurNAc-LAAs are members of the bacterial autolytic system and carry a signal peptide in their N-termini that allows their transport across the cytoplasmic membrane. However, the bacteriophage MurNAc-LAAs are endolysins since these phage-encoded enzymes break down bacterial peptidoglycan at the terminal stage of the phage reproduction cycle. As opposed to autolysins, almost all endolysins have no signal peptides and their translocation through the cytoplasmic membrane is thought to proceed with the help of phage-encoded holin proteins. The amidase catalytic module is fused to another functional module (cell wall binding module or CWBM) either at the N- or C-terminus, which is responsible for high affinity binding of the protein to the cell wall.
pfam01520	Amidase_3	1.59e-30	9	186	1	172	N-acetylmuramoyl-L-alanine amidase. This enzyme domain cleaves the amide bond between N-acetylmuramoyl and L-amino acids in bacterial cell walls.
COG0860	AmiC	2.62e-27	9	187	45	229	N-acetylmuramoyl-L-alanine amidase [Cell wall/membrane/envelope biogenesis].

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
QVK20078.1	1.60e-66	9	300	2	284
AXY24739.1	2.18e-65	199	462	602	883
QUF66404.1	3.37e-58	199	462	27	331
AEK88055.1	4.62e-58	199	462	27	343
AEB23056.1	4.62e-58	199	462	27	343

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
4B8V_A	1.98e-19	303	464	37	218	ChainA, Extracellular Protein 6 [Fulvia fulva],4B9H_A Chain A, Extracellular Protein 6 [Fulvia fulva]
5J72_A	2.54e-15	9	167	454	616	ChainA, Putative N-acetylmuramoyl-L-alanine amidase,autolysin cwp6 [Clostridioides difficile 630],5J72_B Chain B, Putative N-acetylmuramoyl-L-alanine amidase,autolysin cwp6 [Clostridioides difficile 630]
4UZ2_A	6.40e-10	368	412	4	48	Crystalstructure of the N-terminal LysM domains from the putative NlpC/P60 D,L endopeptidase from T. thermophilus [Thermus thermophilus HB8],4UZ2_B Crystal structure of the N-terminal LysM domains from the putative NlpC/P60 D,L endopeptidase from T. thermophilus [Thermus thermophilus HB8],4UZ2_C Crystal structure of the N-terminal LysM domains from the putative NlpC/P60 D,L endopeptidase from T. thermophilus [Thermus thermophilus HB8],4UZ2_D Crystal structure of the N-terminal LysM domains from the putative NlpC/P60 D,L endopeptidase from T. thermophilus [Thermus thermophilus HB8],4UZ3_A Crystal structure of the N-terminal LysM domains from the putative NlpC/P60 D,L endopeptidase from T. thermophilus bound to N-acetyl-chitohexaose [Thermus thermophilus HB8],4UZ3_B Crystal structure of the N-terminal LysM domains from the putative NlpC/P60 D,L endopeptidase from T. thermophilus bound to N-acetyl-chitohexaose [Thermus thermophilus HB8],4UZ3_C Crystal structure of the N-terminal LysM domains from the putative NlpC/P60 D,L endopeptidase from T. thermophilus bound to N-acetyl-chitohexaose [Thermus thermophilus HB8]
4XCM_A	8.61e-09	368	412	4	48	Crystalstructure of the putative NlpC/P60 D,L endopeptidase from T. thermophilus [Thermus thermophilus HB8],4XCM_B Crystal structure of the putative NlpC/P60 D,L endopeptidase from T. thermophilus [Thermus thermophilus HB8]
5EMI_A	1.36e-07	9	186	7	178	ChainA, Cell wall hydrolase/autolysin [Nostoc punctiforme PCC 73102]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
O07532	2.17e-56	200	462	28	350	Peptidoglycan endopeptidase LytF OS=Bacillus subtilis (strain 168) OX=224308 GN=lytF PE=1 SV=2
O31852	5.58e-48	241	462	14	268	D-gamma-glutamyl-meso-diaminopimelic acid endopeptidase CwlS OS=Bacillus subtilis (strain 168) OX=224308 GN=cwlS PE=1 SV=1
P37710	1.39e-39	201	460	431	734	Autolysin OS=Enterococcus faecalis (strain ATCC 700802 / V583) OX=226185 GN=EF_0799 PE=1 SV=2
P39046	4.20e-39	201	464	338	666	Muramidase-2 OS=Enterococcus hirae (strain ATCC 9790 / DSM 20160 / JCM 8729 / LMG 6399 / NBRC 3181 / NCIMB 6459 / NCDO 1258 / NCTC 12367 / WDCM 00089 / R) OX=768486 GN=EHR_05900 PE=1 SV=1
P54421	8.36e-31	288	460	9	190	Probable peptidoglycan endopeptidase LytE OS=Bacillus subtilis (strain 168) OX=224308 GN=lytE PE=1 SV=1

SignalP and Lipop Annotations

This protein is predicted as OTHER

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
1.000068	0.000000	0.000000	0.000000	0.000000	0.000000

TMHMM  Annotations     

There is no transmembrane helices in MGYG000000297_00885.