CAZyme Information: MGYG000000322_02587

Basic Information

• Species: Odoribacter sp900544025
• Lineage: Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Marinifilaceae; Odoribacter; Odoribacter sp900544025
• CAZyme ID: MGYG000000322_02587
• CAZy Family: GH84
• CAZyme Description: O-GlcNAcase

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
840	MGYG000000322_103|CGC1	94945.87	7.3128

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000000322	3470887	MAG	Sweden	Europe

• Gene Location: Start: 5889; End: 8411 Strand: +

Full Sequence      

MRKSIVTLIALTLSCISLYAGEIKVHPIPQQIQGGNQTVQVKEIRLIGGEKADPEAVKKL
QELFKITTDSKAYPVYIGEKGDKAVKKYNSRIPAKDGGYYLNIGDKQMIIAGHEGRGTFY
ALQTLSQLLKQQELPVVEIVDYPSVPYRGTVEGFYGQPWSHRDRISQLNFYGANKLNTYI
YGPKDDPFHSSPNWRQPYPEKEAGQIRELVETAKANKVDFVWAIHPGQDIKWNEADRKLL
LNKFELMYQLGVRSFAVFFDDISGEGTDPNRQADLLNYLHQNFVKVKKDVTPLIMCPTQY
NKSWSDPKPGTYLDILGDKLDPSIRIMWTGDRVISDITTEGLQWINKRLKREAYIWWNFP
VSDYVRDHLLLGRSYGLDTAAGNLMSGFVSNPMDKAEASKIAIYSIADYAWNTPKYNSYQ
NWEDAIRYIMPETAGAFRIFAEHNSDLGKNGHGYRREESVEIKPVADRFLDDLSQGQPDT
KDLARLKIEFIKIEKAPEIIVAGNENPALIEEIRPWLSQFQILGQTGQASLNLLLALNQQ
QPDLVGCWNQYSNLLRLEAQKDNISTKNNQNPYQPGIKTGSLVMQPLVDATLLWNAKQLY
KHLNPGQAKTESRVQTQARLQTDIEQLKQVQLQTADQNIVITPLLEVVKINAGQYFAIEL
PLVVNKGSVQANLNNKEIADWGKIEISEDGKTWQTIDFNLKGTNFTANFENRPVKWVRFT
NAGTSTREFHLRKFILQSEQLSGKGGNSLTALDGDLNTSFVLAGAQTFAKTIDGTPAQVT
PLTAPNPQTNFWVWAIDDQNRKQKLGRVLTAFASFDLPANTTSIEISTPVTIDIHEIIWK

Enzyme Prediction     

No EC number prediction in MGYG000000322_02587.

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GH84	148	441	2.8e-112	0.9932203389830508

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
pfam07555	NAGidase	1.96e-169	148	440	1	293	beta-N-acetylglucosaminidase. This family has previously been described as a hyaluronidase. However, more recently it has been shown that this family has beta-N-acetylglucosaminidase activity.
pfam02838	Glyco_hydro_20b	5.52e-15	22	141	1	123	Glycosyl hydrolase family 20, domain 2. This domain has a zincin-like fold.
COG3525	Chb	1.38e-07	13	215	124	357	N-acetyl-beta-hexosaminidase [Carbohydrate transport and metabolism].
pfam18344	CBM32	6.67e-04	620	660	12	52	Carbohydrate binding module family 32. This domain is found in GH84C present in Clostridium perfringens. GH84C is a beta-N-acetylglucosaminidase. This domain is a family 32 carbohydrate binding module (CBM) which preferentially recognizes the non-reducing terminus of N-acetyllactosamine.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
AUI48059.1	1.69e-276	16	736	17	735
QTO26212.1	4.81e-276	16	736	17	735
QRM68690.1	6.81e-276	16	736	17	735
QCQ48131.1	6.81e-276	16	736	17	735
QLK80951.1	6.81e-276	16	736	17	735

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
2VVN_A	1.30e-271	1	740	1	736	BtGH84in complex with NH-Butylthiazoline [Bacteroides thetaiotaomicron VPI-5482],2VVN_B BtGH84 in complex with NH-Butylthiazoline [Bacteroides thetaiotaomicron VPI-5482],2VVS_A BtGH84 structure in complex with PUGNAc [Bacteroides thetaiotaomicron VPI-5482],2X0H_A BtGH84 Michaelis complex [Bacteroides thetaiotaomicron VPI-5482],2X0H_B BtGH84 Michaelis complex [Bacteroides thetaiotaomicron VPI-5482],4AIS_A A complex structure of BtGH84 [Bacteroides thetaiotaomicron VPI-5482],4AIS_B A complex structure of BtGH84 [Bacteroides thetaiotaomicron VPI-5482],7OU8_AAA Chain AAA, O-GlcNAcase BT_4395 [Bacteroides thetaiotaomicron VPI-5482],7OU8_BBB Chain BBB, O-GlcNAcase BT_4395 [Bacteroides thetaiotaomicron VPI-5482]
2WZI_A	7.44e-271	1	740	1	736	BtGH84D243N in complex with 5F-oxazoline [Bacteroides thetaiotaomicron VPI-5482],2WZI_B BtGH84 D243N in complex with 5F-oxazoline [Bacteroides thetaiotaomicron VPI-5482]
2WZH_A	7.44e-271	1	740	1	736	BtGH84D242N in complex with MeUMB-derived oxazoline [Bacteroides thetaiotaomicron VPI-5482],4AIU_A A complex structure of BtGH84 [Bacteroides thetaiotaomicron VPI-5482]
2J4G_A	3.95e-270	23	740	2	714	Bacteroidesthetaiotaomicron GH84 O-GlcNAcase in complex with n-butyl- thiazoline inhibitor [Bacteroides thetaiotaomicron VPI-5482],2J4G_B Bacteroides thetaiotaomicron GH84 O-GlcNAcase in complex with n-butyl- thiazoline inhibitor [Bacteroides thetaiotaomicron VPI-5482]
2JIW_A	4.09e-270	23	740	3	715	Bacteroidesthetaiotaomicron GH84 O-GlcNAcase in complex with 2- Acetylamino-2-deoxy-1-epivalienamine [Bacteroides thetaiotaomicron VPI-5482],2JIW_B Bacteroides thetaiotaomicron GH84 O-GlcNAcase in complex with 2- Acetylamino-2-deoxy-1-epivalienamine [Bacteroides thetaiotaomicron VPI-5482]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
Q89ZI2	7.14e-271	1	740	1	736	O-GlcNAcase BT_4395 OS=Bacteroides thetaiotaomicron (strain ATCC 29148 / DSM 2079 / JCM 5827 / CCUG 10774 / NCTC 10582 / VPI-5482 / E50) OX=226186 GN=BT_4395 PE=1 SV=1
Q8XL08	2.81e-106	26	536	47	574	O-GlcNAcase NagJ OS=Clostridium perfringens (strain 13 / Type A) OX=195102 GN=nagJ PE=1 SV=1
Q0TR53	2.03e-105	26	536	47	574	O-GlcNAcase NagJ OS=Clostridium perfringens (strain ATCC 13124 / DSM 756 / JCM 1290 / NCIMB 6125 / NCTC 8237 / Type A) OX=195103 GN=nagJ PE=1 SV=1
P26831	3.91e-56	24	758	40	833	Hyaluronoglucosaminidase OS=Clostridium perfringens (strain 13 / Type A) OX=195102 GN=nagH PE=1 SV=2
O60502	9.13e-37	149	409	63	331	Protein O-GlcNAcase OS=Homo sapiens OX=9606 GN=OGA PE=1 SV=2

SignalP and Lipop Annotations

This protein is predicted as SP

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
0.000701	0.705716	0.292852	0.000238	0.000250	0.000225

TMHMM  Annotations     

There is no transmembrane helices in MGYG000000322_02587.