CAZyme Information: MGYG000000338_02052

Basic Information

• Species: Anaerobiospirillum sp900543125
• Lineage: Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales; Succinivibrionaceae; Anaerobiospirillum; Anaerobiospirillum sp900543125
• CAZyme ID: MGYG000000338_02052
• CAZy Family: GT51
• CAZyme Description: Monofunctional biosynthetic peptidoglycan transglycosylase

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
880		97199.69	8.5936

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000000338	3410865	MAG	Sweden	Europe

• Gene Location: Start: 76; End: 2718 Strand: +

Full Sequence      

MNKDGYHRILTTQSQVDPLYIKMLIASEDERFYSHPGVDVISIGRALWSNITSGSIVSGA
STIAMQVCRMLEPKERSIWSKAKEALGALYLTHYYGRDQILSLYLTLAPFGGNIEGVNAA
SYIYFNHSPKNLTPAEAALLVALPRSPEVIRPDRHPKSARYYRNEVLKKAVTDKIIKQDI
LAFATKEPIPNVRHALPQDAYYLGQSIFTSKLITPEIKALRELTACIIGKQVISSEQDIK
SSHSAKDNQDQSPTLGKEDLENNSQELASAKGNTLGQDLSASAQGAQLVVHNQEQKETIN
QVIEVQLSGASIKPDQYEAFNKAALQDGLNKFVNVDSSKFGQGHGNSHGQGQGLSQRSHS
TEFERKLKMLNSLGPLPRELYTTIDPKVQELLINAVEEYRHSFLGEDKQDSLSIVVVDND
TFEVLGYVGALGTDISYVDSALALRSPGSALKPFAYALAFDQGLLHPNSILLDKGQIYDG
YQPRNYDRQFYGELTAAKALQASLNLPAVEVMRAIGPDNFINMINNTKNDIDLYDHSQEL
NSYVHGSLVLPKQSKPSLSLVLGGCGISLFDLTQLYASLAHDGSSAPLRVLSAKRPSSAM
VRKHNYKYHFDAENKKEKTSVRTVGNKPERSYQSLKENHIHYQTMVRPAAARATYNILQG
TPAPFGFGNNYFSLGPKVSYKTGTSYKNRDAWALGSSNNITVGVWTGRNDGSPSYQRTGF
NHAAPILFKVITKLTSNDMRKKRIEDDVLLHARPPMALSKIQVKQLGLVTKKEDGYQSYN
ANLTSDTKDNQVHKFGSKSTIEPLAIAFPTDNSRLSLGSSSEVLIKFSGGKAPYYVLIND
ELNDNLESFKPTHDGFYQITIIDSLGNSVSSQVYVQGLDQ

Enzyme Prediction     

No EC number prediction in MGYG000000338_02052.

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GT51	7	169	8.2e-49	0.9209039548022598

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
COG4953	PbpC	3.67e-151	2	877	52	733	Membrane carboxypeptidase/penicillin-binding protein PbpC [Cell wall/membrane/envelope biogenesis].
TIGR02073	PBP_1c	5.96e-144	3	876	28	727	penicillin-binding protein 1C. This subfamily of the penicillin binding proteins includes the member from E. coli designated penicillin-binding protein 1C. Members have both transglycosylase and transpeptidase domains and are involved in forming cross-links in the late stages of peptidoglycan biosynthesis. All members of this subfamily are presumed to have the same basic function. [Cell envelope, Biosynthesis and degradation of murein sacculus and peptidoglycan]
PRK11240	PRK11240	1.70e-101	2	747	56	576	penicillin-binding protein 1C; Provisional
COG0744	MrcB	2.65e-84	5	737	73	599	Membrane carboxypeptidase (penicillin-binding protein) [Cell wall/membrane/envelope biogenesis].
TIGR02074	PBP_1a_fam	5.92e-80	7	726	1	520	penicillin-binding protein, 1A family. Bacterial that synthesize a cell wall of peptidoglycan (murein) generally have several transglycosylases and transpeptidases for the task. This family consists of bifunctional transglycosylase/transpeptidase penicillin-binding proteins (PBP). In the Proteobacteria, this family includes PBP 1A but not the paralogous PBP 1B (TIGR02071). This family also includes related proteins, often designated PBP 1A, from other bacterial lineages. [Cell envelope, Biosynthesis and degradation of murein sacculus and peptidoglycan]

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
BAQ16181.1	4.91e-96	4	876	48	675
AUN31587.1	2.52e-95	4	876	47	668
ARQ01853.1	3.45e-94	3	876	58	687
BBK44303.1	2.62e-92	4	876	58	688
AMJ63698.1	1.23e-91	1	876	53	726

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
4OON_A	1.62e-26	20	524	51	514	Crystalstructure of PBP1a in complex with compound 17 ((4Z,8S,11E,14S)-5-(2-amino-1,3-thiazol-4-yl)-14-(5,6-dihydroxy-1,3-dioxo-1,3-dihydro-2H-isoindol-2-yl)-8-formyl-2-methyl-6-oxo-3,10-dioxa-4,7,11-triazatetradeca-4,11-diene-2,12,14-tricarboxylic acid) [Pseudomonas aeruginosa PAO1]
5FGZ_A	4.08e-25	4	189	152	342	E.coli PBP1b in complex with FPI-1465 [Escherichia coli K-12],5HL9_A E. coli PBP1b in complex with acyl-ampicillin and moenomycin [Escherichia coli K-12],5HLA_A E. coli PBP1b in complex with acyl-cephalexin and moenomycin [Escherichia coli K-12],5HLB_A E. coli PBP1b in complex with acyl-aztreonam and moenomycin [Escherichia coli K-12],5HLD_A E. coli PBP1b in complex with acyl-CENTA and moenomycin [Escherichia coli K-12],6YN0_A Structure of E. coli PBP1b with a FtsN peptide activating transglycosylase activity [Escherichia coli K-12],7LQ6_A Chain A, Penicillin-binding protein 1B [Escherichia coli K-12]
3VMA_A	4.28e-25	4	189	173	363	CrystalStructure of the Full-Length Transglycosylase PBP1b from Escherichia coli [Escherichia coli K-12]
3UDF_A	2.09e-24	15	516	46	492	ChainA, Penicillin-binding protein 1a [Acinetobacter baumannii],3UDF_B Chain B, Penicillin-binding protein 1a [Acinetobacter baumannii],3UDI_A Chain A, Penicillin-binding protein 1a [Acinetobacter baumannii],3UDI_B Chain B, Penicillin-binding protein 1a [Acinetobacter baumannii],3UDX_A Chain A, Penicillin-binding protein 1a [Acinetobacter baumannii],3UDX_B Chain B, Penicillin-binding protein 1a [Acinetobacter baumannii],3UE0_A Chain A, Penicillin-binding protein 1a [Acinetobacter baumannii],3UE0_B Chain B, Penicillin-binding protein 1a [Acinetobacter baumannii],3UE1_A Chain A, Penicillin-binding protein 1a [Acinetobacter baumannii],3UE1_B Chain B, Penicillin-binding protein 1a [Acinetobacter baumannii]
3FWL_A	3.81e-24	4	189	156	346	CrystalStructure of the Full-Length Transglycosylase PBP1b from Escherichia coli [Escherichia coli]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
P76577	4.43e-68	4	736	60	566	Penicillin-binding protein 1C OS=Escherichia coli (strain K12) OX=83333 GN=pbpC PE=1 SV=1
Q92G78	8.42e-34	8	727	67	709	Penicillin-binding protein 1A OS=Rickettsia conorii (strain ATCC VR-613 / Malish 7) OX=272944 GN=mrcA PE=3 SV=1
Q4UK08	8.42e-34	8	782	67	756	Penicillin-binding protein 1A OS=Rickettsia felis (strain ATCC VR-1525 / URRWXCal2) OX=315456 GN=mrcA PE=3 SV=1
Q68VU2	1.92e-33	8	727	67	709	Penicillin-binding protein 1A OS=Rickettsia typhi (strain ATCC VR-144 / Wilmington) OX=257363 GN=mrcA PE=3 SV=1
Q9ZCE9	4.51e-33	8	727	67	709	Penicillin-binding protein 1A OS=Rickettsia prowazekii (strain Madrid E) OX=272947 GN=mrcA PE=3 SV=1

SignalP and Lipop Annotations

This protein is predicted as OTHER

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
1.000048	0.000000	0.000000	0.000000	0.000000	0.000000

TMHMM  Annotations     

There is no transmembrane helices in MGYG000000338_02052.