CAZyme Information: MGYG000000339_01022

Basic Information

• Lineage: Bacteria; Firmicutes_A; Clostridia; Oscillospirales; Oscillospiraceae; UBA9475
• CAZyme ID: MGYG000000339_01022
• CAZy Family: GH2
• CAZyme Description: Beta-galactosidase

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
1098		119251.05	4.2852

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000000339	1808008	MAG	Sweden	Europe

• Gene Location: Start: 47; End: 3343 Strand: +

Full Sequence      

MNATVYLNGHELGFHPYGYTSFAFELPQEYLNYEGDNVLAVKVEHQTPSSRWYSGSGIYR
SVHLTVTDPVHVDRNGTYITTPNLADSQGKDGTVNVVTTVANQSGEAANVVVRQSIYELG
GEQSVASVTGETVTVDANSTKDVTLTTKVDSPKLWDTDNPNLYTAVTEVLVDDQVVDTYT
TEFGFRWLTFNNDSTGFELNGVPTKLKGVCMHHDQGALGSEAWCRAIERQVEILKEMGCN
AIRVTHNPAADELVEIANRHGIMLIDEAFDTWTNPKNGNTQDYSKWFNVTIEEGNNILGG
ETGMTWAEYDVKAMAERDKNAPSVIMYSLGNEIFEGLSHANVSNYPQIARDLCTWLQEVD
TTRPPTFGQNSNTDSIAQQVAAVLNEFGGITGLNYAGSSRYDQWVNRGLKVYYSETASAV
NSRGVYDRKANNNDGGKGDYLLTSYDKSAVGWGALASDAWWRVLERDTSMGEFVWTGFDY
IGEPTPWNGTGAGAVGTWPKSPKSSYFGIIDTNGLPKDSYYLYQSMWNEDVTTLHVLPTW
NREDIVVDGNGKVEVVVYSDAPQVKVYLNGEEVGTAKTTETTTNAGYTYRTFTSGTGAYT
QNSGHQSLYATFMVPYAEGTLEVKAFDAEGNQITDTEGRSVVKTTSSASQLTLSADRDSI
VADGDDLSYVTIDVKDTNGNLVNTDDVNVQLSIEGEGTIVGVDNGRQPDHTSYQSLSRNV
GAGQLVAIVQSTDKAGEFTVTATADGLKAGSVTVTTTPVENEGGNNPVVSYDISRYHYVK
VGNAPELPDTVVVTYKDGTTKELGVTWNDYNEDLIQEVGAFTISGVIDEVNVTTTVNVNM
LDQVAALLNYSAAVRKDGQITLPASRPAVLADGTILNAEFPVEWNIPEDLSATVGTKVVQ
GISQVFGEEITVTASIRVAEGDVTIGGNVASVAADLTQSIPENLQSDTLEAIRNGSTEVD
PNLDGGPNESTWSNYDSAQAGNKSASITFRYDTAQFLGQVVLFFATDSFSAALPSDVQME
VSQNGEQWTTLDITKGEATNPHNNVTRIRSHRPSGCRVLPPDGHQQGGRSRFRRFLLLRQ
PHRGGAECGSDQLPHQQL

Enzyme Prediction     

EC	3.2.1.23

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GH2	2	675	8.6e-97	0.6502659574468085

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
COG3250	LacZ	1.89e-62	3	664	93	676	Beta-galactosidase/beta-glucuronidase [Carbohydrate transport and metabolism].
pfam18565	Glyco_hydro2_C5	3.12e-37	651	753	1	103	Glycoside hydrolase family 2 C-terminal domain 5. Domain 5 is found in dimeric beta-D-galactosidase from Paracoccus sp. 32d, which contributes to stabilization of the functional dimer. It is suggested that the location of this domain 5, may be one of the factors responsible for the creation of a functional dimer and cold-adaptation of this enzyme.
PRK10150	PRK10150	9.51e-36	2	367	94	447	beta-D-glucuronidase; Provisional
PRK10340	ebgA	4.07e-28	5	363	141	470	cryptic beta-D-galactosidase subunit alpha; Reviewed
PRK09525	lacZ	5.35e-23	5	364	153	484	beta-galactosidase.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
QRT48501.1	0.0	1	1035	148	1186
ASM70369.1	0.0	1	1033	170	1200
BAI94821.1	0.0	1	1035	148	1188
ALE10930.1	0.0	1	1035	148	1188
AFL04054.1	0.0	1	1035	148	1188

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
7NIT_A	0.0	1	1035	116	1156	ChainA, Beta-galactosidase [Bifidobacterium bifidum],7NIT_B Chain B, Beta-galactosidase [Bifidobacterium bifidum],7NIT_C Chain C, Beta-galactosidase [Bifidobacterium bifidum],7NIT_D Chain D, Beta-galactosidase [Bifidobacterium bifidum],7NIT_E Chain E, Beta-galactosidase [Bifidobacterium bifidum],7NIT_F Chain F, Beta-galactosidase [Bifidobacterium bifidum]
5DMY_A	1.46e-254	1	778	122	904	Beta-galactosidase- construct 33-930 [Bifidobacterium bifidum],5DMY_B Beta-galactosidase - construct 33-930 [Bifidobacterium bifidum],5DMY_C Beta-galactosidase - construct 33-930 [Bifidobacterium bifidum]
6QUB_B	2.49e-251	1	759	93	856	Truncatedbeta-galactosidase III from Bifidobacterium bifidum in complex with galactose [Bifidobacterium bifidum]
6QUB_A	6.27e-251	1	759	116	879	Truncatedbeta-galactosidase III from Bifidobacterium bifidum in complex with galactose [Bifidobacterium bifidum],6QUC_A Truncated beta-galactosidase III from Bifidobacterium bifidum [Bifidobacterium bifidum],6QUC_B Truncated beta-galactosidase III from Bifidobacterium bifidum [Bifidobacterium bifidum],6QUD_A 2-deoxy-galactose reaction intermediate of a Truncated beta-galactosidase III from Bifidobacterium bifidum [Bifidobacterium bifidum]
7CWD_A	3.25e-172	1	757	92	802	ChainA, beta-glalactosidase [Niallia circulans],7CWI_A Chain A, beta-galactosidase [Niallia circulans]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
A7LXS9	1.82e-121	1	748	130	840	Beta-galactosidase BoGH2A OS=Bacteroides ovatus (strain ATCC 8483 / DSM 1896 / JCM 5824 / BCRC 10623 / CCUG 4943 / NCTC 11153) OX=411476 GN=BACOVA_02645 PE=1 SV=1
T2KM09	2.11e-61	1	712	134	775	Putative beta-glucuronidase OS=Formosa agariphila (strain DSM 15362 / KCTC 12365 / LMG 23005 / KMM 3901 / M-2Alg 35-1) OX=1347342 GN=BN863_22050 PE=2 SV=2
T2KPJ7	8.71e-33	1	577	131	676	Putative beta-glucuronidase OS=Formosa agariphila (strain DSM 15362 / KCTC 12365 / LMG 23005 / KMM 3901 / M-2Alg 35-1) OX=1347342 GN=BN863_21970 PE=2 SV=1
P77989	3.74e-29	3	738	86	719	Beta-galactosidase OS=Thermoanaerobacter pseudethanolicus (strain ATCC 33223 / 39E) OX=340099 GN=lacZ PE=3 SV=2
O52847	4.63e-23	5	363	165	502	Beta-galactosidase OS=Priestia megaterium (strain DSM 319 / IMG 1521) OX=592022 GN=bgaM PE=3 SV=1

SignalP and Lipop Annotations

This protein is predicted as OTHER

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
1.000030	0.000001	0.000000	0.000000	0.000000	0.000000

TMHMM  Annotations     

There is no transmembrane helices in MGYG000000339_01022.