CAZyme Information: MGYG000000342_00665

Basic Information

• Lineage: Bacteria; Proteobacteria; Gammaproteobacteria; Burkholderiales; Burkholderiaceae; Oxalobacter
• CAZyme ID: MGYG000000342_00665
• CAZy Family: GH23
• CAZyme Description: Membrane-bound lytic murein transglycosylase C

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
297		32942.9	10.4303

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000000342	990972	MAG	Sweden	Europe

• Gene Location: Start: 651; End: 1544 Strand: -

Full Sequence      

MFHSFFKQFDLHHLRNRLWHPISTRSIQASLTSNPVIWMFFISLFFVILTLCCIRPDLFK
TVGNSLPIHEAHAAESRTISPDSANLLPVNAIAENEHHFKLDELKQKQRVAAWIAKRYRI
AGAASNLFVSTAYKTAFDLGLDPHLILAVMAIESRFNPYAESAVGAQGLMQVMSRVHADK
FADHGGIQNALDPVVNIKVGSRILRDYVRQKGSVELALKSYVGAAAMSHDGGYGSRVLSE
YRMLKAVASGKRGPSLIHAKSPVKQRATRTTKTAGDTTLSRQKTAESRQDGNTETML

Enzyme Prediction     

No EC number prediction in MGYG000000342_00665.

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GH23	143	244	1.7e-18	0.8

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
cd00254	LT-like	2.32e-21	143	242	1	100	lytic transglycosylase(LT)-like domain. Members include the soluble and insoluble membrane-bound LTs in bacteria and LTs in bacteriophage lambda. LTs catalyze the cleavage of the beta-1,4-glycosidic bond between N-acetylmuramic acid (MurNAc) and N-acetyl-D-glucosamine (GlcNAc), as do "goose-type" lysozymes. However, in addition to this, they also make a new glycosidic bond with the C6 hydroxyl group of the same muramic acid residue.
pfam01464	SLT	1.90e-19	140	221	9	92	Transglycosylase SLT domain. This family is distantly related to pfam00062. Members are found in phages, type II, type III and type IV secretion systems.
cd16896	LT_Slt70-like	6.30e-18	140	221	16	102	uncharacterized lytic transglycosylase subfamily with similarity to Slt70. Uncharacterized lytic transglycosylase (LT) with a conserved sequence pattern suggesting similarity to the Slt70, a 70kda soluble lytic transglycosylase which also has an N-terminal U-shaped U-domain and a linker L-domain. LTs catalyze the cleavage of the beta-1,4-glycosidic bond between N-acetylmuramic acid (MurNAc) and N-acetyl-D-glucosamine (GlcNAc), as do "goose-type" lysozymes. However, in addition to this, they also make a new glycosidic bond with the C6 hydroxyl group of the same muramic acid residue.
cd13401	Slt70-like	9.73e-18	136	221	14	104	70kDa soluble lytic transglycosylase (Slt70) and similar proteins. Catalytic domain of the 70kda soluble lytic transglycosylase (LT)-like proteins, which also have an N-terminal U-shaped U-domain and a linker L-domain. LTs catalyze the cleavage of the beta-1,4-glycosidic bond between N-acetylmuramic acid (MurNAc) and N-acetyl-D-glucosamine (GlcNAc), as do "goose-type" lysozymes. However, in addition to this, they also make a new glycosidic bond with the C6 hydroxyl group of the same muramic acid residue. Proteins similar to this family include the soluble and insoluble membrane-bound LTs in bacteria and the LTs in bacteriophage lambda.
COG0741	MltE	1.18e-17	72	221	76	237	Soluble lytic murein transglycosylase and related regulatory proteins (some contain LysM/invasin domains) [Cell wall/membrane/envelope biogenesis].

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
ARQ45275.1	7.24e-105	1	288	1	287
QDX33894.1	7.24e-105	1	288	1	287
ARQ77567.1	7.24e-105	1	288	1	287
AVR96126.1	4.42e-65	54	262	57	273
AXA91692.1	1.76e-64	55	262	58	273

PDB Hits     

has no PDB hit.

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
P27380	5.54e-09	141	244	20	122	Transglycosylase OS=Enterobacteria phage PRD1 OX=10658 GN=VII PE=1 SV=3
O31976	5.32e-08	122	221	1416	1511	SPbeta prophage-derived uncharacterized transglycosylase YomI OS=Bacillus subtilis (strain 168) OX=224308 GN=yomI PE=3 SV=2
O64046	5.32e-08	122	221	1416	1511	Probable tape measure protein OS=Bacillus phage SPbeta OX=66797 GN=yomI PE=3 SV=1

SignalP and Lipop Annotations

This protein is predicted as OTHER

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
1.000025	0.000027	0.000000	0.000000	0.000000	0.000000

TMHMM  Annotations     

start	end
35	54