CAZyme Information: MGYG000000343_01860

Basic Information

• Species: Odoribacter sp900548135
• Lineage: Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Marinifilaceae; Odoribacter; Odoribacter sp900548135
• CAZyme ID: MGYG000000343_01860
• CAZy Family: GH2
• CAZyme Description: Beta-galactosidase

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
852		98865.31	7.317

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000000343	3841231	MAG	Sweden	Europe

• Gene Location: Start: 43335; End: 45893 Strand: -

Full Sequence      

MKIVLLLLILLYWGVGRGQKKAETILLNQNWYFAQDGKGDWLEAEVPGTIHQDLIRHQLL
PDPFYGINEEKIQWVEDKDWEYKTVFIVTEEQLGRDAAILNLKGLDTYADVYLNGALILQ
SDNMFVGHQVPVKDKLRKGENYLHVYFHSPIKQTLPQWRSNGFNYPADNDHRAERLSVFT
RKAPYSYGWDWGIRMVTCGIWRPVVLTFQDVVSIEDFYVQQLELKTEKAVLVANLQLKSL
TTFPRTVKVKVSCTTPAGKHQSVEQEMILKNGEEHFHIPVEIQHPELWMPNGWGKPALYN
FRVQIDFEGLTVAEQSQRIGLRTIRVVQEKDSIGESFYFEVNGVPMFAKGANYIPADAML
PAITTARYETLFRDIREANMNMIRVWGGGTYEDDRFYELADENGILIWQDFMFGCTTYPA
DAVFLRRVEEEAAYNIRRLRHHACLAMWCGNNEIKEGLLYWGWKKKYGPEIYQEMCEGYE
RIFEQLLPAKVKELDSGRFYMHGSPYFANWGRPESWKVGDSHNWGVWYGRKPFESLDAEI
PRFMSEFGFQSFPEMKTIASFAAEGDYQLESQVMNAHQKSTIGNALIRTYMERDYILPKK
FEDFVYIGLVMQGHGIRRGLEAHRRNRPYCMGTLYWQLNDSWPVVSWSGIDYFGNWKALH
YQAKRAFAPLKMNAIQVGDSLQIWLLSDRLEKLNGLTLELQRTDFYGKVQKKQVIRVEAA
ANCSRMVHQMALSDWGIPGQTHKCYLLLTLKDKAGKKIDEEPYFFSRPRELDLPQPHLHW
QVKTMDGQCEVTLSSRELVKDVFVEVPAQGARFSDNFFDLLPGTTRKITITSALFQKGKS
PGIKIKHLKSIY

Enzyme Prediction     

No EC number prediction in MGYG000000343_01860.

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GH2	19	730	9.6e-106	0.6981382978723404

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
COG3250	LacZ	1.65e-110	19	852	7	806	Beta-galactosidase/beta-glucuronidase [Carbohydrate transport and metabolism].
pfam17753	Ig_mannosidase	1.79e-16	775	848	1	76	Ig-fold domain. This Ig-like fold domain is found in mannosidase enzymes.
pfam00703	Glyco_hydro_2	4.00e-15	212	322	1	106	Glycosyl hydrolases family 2. This family contains beta-galactosidase, beta-mannosidase and beta-glucuronidase activities.
pfam17786	Mannosidase_ig	1.13e-13	679	770	1	91	Mannosidase Ig/CBM-like domain. This domain corresponds to domain 4 in the structure of Bacteroides thetaiotaomicron beta-mannosidase, BtMan2A. This domain has an Ig-like fold.
PRK10150	PRK10150	0.001	280	498	237	443	beta-D-glucuronidase; Provisional

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
QRP90544.1	0.0	16	852	18	855
QCQ41352.1	0.0	16	852	18	855
QKH84210.1	0.0	14	852	14	855
CUA18251.1	0.0	16	852	18	855
BAD48480.1	0.0	16	852	18	855

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
2VJX_A	0.0	17	852	1	838	Structuraland biochemical evidence for a boat-like transition state in beta-mannosidases [Bacteroides thetaiotaomicron VPI-5482],2VJX_B Structural and biochemical evidence for a boat-like transition state in beta-mannosidases [Bacteroides thetaiotaomicron VPI-5482],2VL4_A Structural and biochemical evidence for a boat-like transition state in beta-mannosidases [Bacteroides thetaiotaomicron VPI-5482],2VL4_B Structural and biochemical evidence for a boat-like transition state in beta-mannosidases [Bacteroides thetaiotaomicron VPI-5482],2VMF_A Structural and biochemical evidence for a boat-like transition state in beta-mannosidases [Bacteroides thetaiotaomicron VPI-5482],2VMF_B Structural and biochemical evidence for a boat-like transition state in beta-mannosidases [Bacteroides thetaiotaomicron VPI-5482],2VO5_A Structural and biochemical evidence for a boat-like transition state in beta-mannosidases [Bacteroides thetaiotaomicron VPI-5482],2VO5_B Structural and biochemical evidence for a boat-like transition state in beta-mannosidases [Bacteroides thetaiotaomicron VPI-5482],2VOT_A Structural and biochemical evidence for a boat-like transition state in beta-mannosidases [Bacteroides thetaiotaomicron VPI-5482],2VOT_B Structural and biochemical evidence for a boat-like transition state in beta-mannosidases [Bacteroides thetaiotaomicron VPI-5482],2VQT_A Structural and biochemical evidence for a boat-like transition state in beta-mannosidases [Bacteroides thetaiotaomicron],2VQT_B Structural and biochemical evidence for a boat-like transition state in beta-mannosidases [Bacteroides thetaiotaomicron],2VR4_A Transition-state mimicry in mannoside hydrolysis: characterisation of twenty six inhibitors and insight into binding from linear free energy relationships and 3-D structure [Bacteroides thetaiotaomicron VPI-5482],2VR4_B Transition-state mimicry in mannoside hydrolysis: characterisation of twenty six inhibitors and insight into binding from linear free energy relationships and 3-D structure [Bacteroides thetaiotaomicron VPI-5482]
7OP6_A	0.0	15	852	1	840	ChainA, Beta-mannosidase [Bacteroides thetaiotaomicron VPI-5482],7OP6_B Chain B, Beta-mannosidase [Bacteroides thetaiotaomicron VPI-5482],7OP7_A Chain A, Beta-mannosidase [Bacteroides thetaiotaomicron VPI-5482],7OP7_B Chain B, Beta-mannosidase [Bacteroides thetaiotaomicron VPI-5482]
2WBK_A	0.0	17	852	1	838	Structureof the Michaelis complex of beta-mannosidase, Man2A, provides insight into the conformational itinerary of mannoside hydrolysis [Bacteroides thetaiotaomicron VPI-5482],2WBK_B Structure of the Michaelis complex of beta-mannosidase, Man2A, provides insight into the conformational itinerary of mannoside hydrolysis [Bacteroides thetaiotaomicron VPI-5482]
2JE8_A	0.0	15	852	1	840	Structureof a beta-mannosidase from Bacteroides thetaiotaomicron [Bacteroides thetaiotaomicron VPI-5482],2JE8_B Structure of a beta-mannosidase from Bacteroides thetaiotaomicron [Bacteroides thetaiotaomicron VPI-5482]
2VQU_A	0.0	17	852	1	838	Structuraland biochemical evidence for a boat-like transition state in beta-mannosidases [Bacteroides thetaiotaomicron],2VQU_B Structural and biochemical evidence for a boat-like transition state in beta-mannosidases [Bacteroides thetaiotaomicron]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
Q0CCA0	2.60e-130	27	848	9	841	Beta-mannosidase B OS=Aspergillus terreus (strain NIH 2624 / FGSC A1156) OX=341663 GN=mndB PE=3 SV=2
A1D911	6.09e-128	21	842	3	834	Beta-mannosidase B OS=Neosartorya fischeri (strain ATCC 1020 / DSM 3700 / CBS 544.65 / FGSC A1164 / JCM 1740 / NRRL 181 / WB 181) OX=331117 GN=mndB PE=3 SV=1
Q4WAH4	1.20e-127	21	842	3	834	Beta-mannosidase B OS=Neosartorya fumigata (strain ATCC MYA-4609 / Af293 / CBS 101355 / FGSC A1100) OX=330879 GN=mndB PE=3 SV=1
B0YBU9	4.61e-127	21	842	3	834	Beta-mannosidase B OS=Neosartorya fumigata (strain CEA10 / CBS 144.89 / FGSC A1163) OX=451804 GN=mndB PE=3 SV=1
I2C092	1.23e-125	24	851	6	855	Beta-mannosidase B OS=Thermothelomyces thermophilus OX=78579 GN=man9 PE=1 SV=1

SignalP and Lipop Annotations

This protein is predicted as SP

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
0.000278	0.999149	0.000168	0.000135	0.000132	0.000127

TMHMM  Annotations     

There is no transmembrane helices in MGYG000000343_01860.