CAZyme Information: MGYG000000360_03076

Basic Information

• Species: UBA1829 sp900548385
• Lineage: Bacteria; Verrucomicrobiota; Lentisphaeria; Victivallales; UBA1829; UBA1829; UBA1829 sp900548385
• CAZyme ID: MGYG000000360_03076
• CAZy Family: PL8
• CAZyme Description: hypothetical protein

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
847		94258.1	10.3102

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000000360	4492877	MAG	Sweden	Europe

• Gene Location: Start: 209629; End: 212172 Strand: -

Full Sequence      

MNQINFRHLAATGGLAVAVMTIQAFAPLSAAAQSPLSGIPASQLRWRSVPGVTVTGNRVR
VRIPAGSPASRQRNCARSNFDITPFQNRELGISIQMRGKNVSPSRRRWSYNARLQLSYRL
PNGSMRWVEAVTPSGNFNWTSVQLSDWIPAGSSGGIIYLGLDNGSGEIEFNLSTLKISAL
GEEAEQPEAIAPVLARELELIESRMRRMLLSGRIKPEKEVLALIARQRADGTFDGIDYSN
ENRSQWKTAEHLSQTRAIARAYASENHPLYRDAAVGKAIRNAVNWWSSKRPFNSNWWWND
MWVPEALGETLLLASDLFPDGPERSAALRVCRQAKFLPRYTGNNRVYIARNIFLRALLER
NTGPLNKAAAVLSEEIRFAAVENKTKDSFGGIRADGCYHLHGPQVQFGNYGCEFLKNVAN
WANIWRDTHWQFSPEQWEIIRHLAFNGFRWVLWRGRMDLLACGRQLGRNAPAVKGKATLA
AFRLLREADQNHREAYDEVLDQSVDNTDRLVGNRHFWNSDYMLHRRPGWYAAVRMNSVRV
RPIEDGINWDNALGRYFSDGVCLITRTGREYDNITAVWNWTRLPGTTLPATPVTSTRQLR
WTLRPQGRSRLLGESGFVGGVTDGICGVAVFTMNLDGVKAKKAYFFDSDSVRQLVCGINS
TSPFEVATTVNSSLRNGEIRQGPGWFWHDGIGYRGSGMKLTAGVRTGDWRYLEGGLSEPM
PVSRELFTLSIEHGLAPRGASCEYTILPGATPETTAAWQGGKTLANTPQLQAVELRDGRI
GAVFHAPGRLGTFRTDSPGVFLIGKKEVWAADPAARLNRMKLILGGVVKQLSLPGGEMAG
SSVIVKF

Enzyme Prediction     

No EC number prediction in MGYG000000360_03076.

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
PL8	514	749	2.6e-51	0.9879518072289156

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
cd01083	GAG_Lyase	2.88e-103	227	813	32	693	Glycosaminoglycan (GAG) polysaccharide lyase family. This family consists of a group of secreted bacterial lyase enzymes capable of acting on glycosaminoglycans, such as hyaluronan and chondroitin, in the extracellular matrix of host tissues, contributing to the invasive capacity of the pathogen. These are broad-specificity glycosaminoglycan lyases which recognize uronyl residues in polysaccharides and cleave their glycosidic bonds via a beta-elimination reaction to form a double bond between C-4 and C-5 of the non-reducing terminal uronyl residues of released products. Substrates include chondroitin, chondroitin 4-sulfate, chondroitin 6-sulfate, and hyaluronic acid. Family members include chondroitin AC lyase, chondroitin abc lyase, xanthan lyase, and hyalurate lyase.
pfam02278	Lyase_8	5.84e-61	513	751	1	252	Polysaccharide lyase family 8, super-sandwich domain. This family consists of a group of secreted bacterial lyase enzymes EC:4.2.2.1 capable of acting on hyaluronan and chondroitin in the extracellular matrix of host tissues, contributing to the invasive capacity of the pathogen.
pfam08124	Lyase_8_N	7.15e-30	239	500	51	321	Polysaccharide lyase family 8, N terminal alpha-helical domain. This family consists of a group of secreted bacterial lyase enzymes EC:4.2.2.1 capable of acting on hyaluronan and chondroitin in the extracellular matrix of host tissues, contributing to the invasive capacity of the pathogen.
pfam02884	Lyase_8_C	1.42e-05	764	813	1	59	Polysaccharide lyase family 8, C-terminal beta-sandwich domain. This family consists of a group of secreted bacterial lyase enzymes EC:4.2.2.1 capable of acting on hyaluronan and chondroitin in the extracellular matrix of host tissues, contributing to the invasive capacity of the pathogen.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
AVM45527.1	0.0	59	847	51	848
AQT67414.1	1.70e-114	228	826	67	678
QGY42584.1	1.01e-95	217	843	52	686
ACB77597.1	1.44e-94	225	843	71	688
QBG49151.1	2.21e-92	228	846	63	654

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
1CB8_A	4.19e-84	230	846	38	678	CHONDROITINASEAC LYASE FROM FLAVOBACTERIUM HEPARINUM [Pedobacter heparinus]
1HM2_A	6.81e-84	230	846	60	700	ACTIVESITE OF CHONDROITINASE AC LYASE REVEALED BY THE STRUCTURE OF ENZYME-OLIGOSACCHARIDE COMPLEXES AND MUTAGENESIS [Pedobacter heparinus],1HM3_A Active Site Of Chondroitinase Ac Lyase Revealed By The Structure Of Enzyme-Oligosaccharide Complexes And Mutagenesis [Pedobacter heparinus],1HMU_A ACTIVE SITE OF CHONDROITINASE AC LYASE REVEALED BY THE STRUCTURE OF ENZYME-OLIGOSACCHARIDE COMPLEXES AND MUTAGENESIS [Pedobacter heparinus],1HMW_A Active Site Of Chondroitinase Ac Lyase Revealed By The Structure Of Enzyme-oligosaccharide Complexes And Mutagenesis [Pedobacter heparinus]
2WCO_A	5.86e-20	341	806	191	700	Structuresof the Streptomyces coelicolor A3(2) Hyaluronan Lyase in Complex with Oligosaccharide Substrates and an Inhibitor [Streptomyces coelicolor A3(2)],2WDA_A The X-ray structure of the Streptomyces coelicolor A3 Chondroitin AC Lyase in Complex with Chondroitin sulphate [Streptomyces violaceoruber]
2X03_A	7.03e-19	341	806	191	700	TheX-ray structure of the Streptomyces coelicolor A3 Chondroitin AC Lyase Y253A mutant [Streptomyces coelicolor A3(2)],2X03_B The X-ray structure of the Streptomyces coelicolor A3 Chondroitin AC Lyase Y253A mutant [Streptomyces coelicolor A3(2)]
1HN0_A	2.83e-07	391	753	491	884	CRYSTALSTRUCTURE OF CHONDROITIN ABC LYASE I FROM PROTEUS VULGARIS AT 1.9 ANGSTROMS RESOLUTION [Proteus vulgaris]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
Q59288	3.73e-83	230	846	60	700	Chondroitinase-AC OS=Pedobacter heparinus (strain ATCC 13125 / DSM 2366 / CIP 104194 / JCM 7457 / NBRC 12017 / NCIMB 9290 / NRRL B-14731 / HIM 762-3) OX=485917 GN=cslA PE=1 SV=1
P59807	2.04e-06	391	753	491	884	Chondroitin sulfate ABC endolyase OS=Proteus vulgaris OX=585 PE=1 SV=2

SignalP and Lipop Annotations

This protein is predicted as SP

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
0.002516	0.995963	0.000588	0.000479	0.000243	0.000201

TMHMM  Annotations     

There is no transmembrane helices in MGYG000000360_03076.