CAZyme Information: MGYG000000361_01557

Basic Information

• Lineage: Bacteria; Firmicutes_A; Clostridia; Lachnospirales; Lachnospiraceae; VSOB01
• CAZyme ID: MGYG000000361_01557
• CAZy Family: GH2
• CAZyme Description: Beta-galactosidase large subunit

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
258		30341.82	4.4942

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000000361	1916694	MAG	Sweden	Europe

• Gene Location: Start: 48; End: 824 Strand: +

Full Sequence      

MRRDMKLFLDNNINAVRTCHYPDQLIWYDMCDENGIYMMAETNLESHGSWQKMGAVEPSY
NVPGSLPQWQEAVLDRARSNFETFKNHASILFWSLGNESYAEKDIEEMNRYLKEKQDGRL
VHYEGVFHNRAYEDTISDVESRMYAPPAEVREYLENAPKKPYLLCEYMHDMGNSLGGMAS
YMKFFDEFEGYHGGFIWDFIDQALWVTDEVTGQRVLRYGGDFDDRPSDYEFSGNGIVFAD
RTEKPAMQEVRYYYGLYK

Enzyme Prediction     

EC	3.2.1.23

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
pfam02836	Glyco_hydro_2_C	4.91e-114	1	258	38	301	Glycosyl hydrolases family 2, TIM barrel domain. This family contains beta-galactosidase, beta-mannosidase and beta-glucuronidase activities.
PRK10340	ebgA	6.64e-67	1	251	357	596	cryptic beta-D-galactosidase subunit alpha; Reviewed
PRK09525	lacZ	8.45e-61	1	252	373	623	beta-galactosidase.
COG3250	LacZ	5.38e-54	1	258	323	557	Beta-galactosidase/beta-glucuronidase [Carbohydrate transport and metabolism].
PRK10150	PRK10150	1.08e-04	1	220	315	569	beta-D-glucuronidase; Provisional

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
AQP40966.1	1.74e-150	1	258	376	633
QNM02889.1	1.36e-148	1	255	379	633
VCV22603.1	9.12e-148	1	258	376	633
CBL13227.1	9.12e-148	1	258	376	633
CBL10015.1	9.12e-148	1	258	376	633

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
6S6Z_A	7.76e-60	1	254	349	593	Structureof beta-Galactosidase from Thermotoga maritima [Thermotoga maritima MSB8],6S6Z_B Structure of beta-Galactosidase from Thermotoga maritima [Thermotoga maritima MSB8],6S6Z_C Structure of beta-Galactosidase from Thermotoga maritima [Thermotoga maritima MSB8],6S6Z_D Structure of beta-Galactosidase from Thermotoga maritima [Thermotoga maritima MSB8],6S6Z_E Structure of beta-Galactosidase from Thermotoga maritima [Thermotoga maritima MSB8],6S6Z_F Structure of beta-Galactosidase from Thermotoga maritima [Thermotoga maritima MSB8],6S6Z_G Structure of beta-Galactosidase from Thermotoga maritima [Thermotoga maritima MSB8],6S6Z_H Structure of beta-Galactosidase from Thermotoga maritima [Thermotoga maritima MSB8]
6SD0_A	7.78e-60	1	254	350	594	Structureof beta-galactosidase from Thermotoga maritima. [Thermotoga maritima MSB8],6SD0_B Structure of beta-galactosidase from Thermotoga maritima. [Thermotoga maritima MSB8],6SD0_C Structure of beta-galactosidase from Thermotoga maritima. [Thermotoga maritima MSB8],6SD0_D Structure of beta-galactosidase from Thermotoga maritima. [Thermotoga maritima MSB8]
6CVM_A	1.94e-52	1	252	371	621	Atomicresolution cryo-EM structure of beta-galactosidase [Escherichia coli K-12],6CVM_B Atomic resolution cryo-EM structure of beta-galactosidase [Escherichia coli K-12],6CVM_C Atomic resolution cryo-EM structure of beta-galactosidase [Escherichia coli K-12],6CVM_D Atomic resolution cryo-EM structure of beta-galactosidase [Escherichia coli K-12],6X1Q_A 1.8 Angstrom resolution structure of b-galactosidase with a 200 kV cryoARM electron microscope [Escherichia coli K-12],6X1Q_B 1.8 Angstrom resolution structure of b-galactosidase with a 200 kV cryoARM electron microscope [Escherichia coli K-12],6X1Q_C 1.8 Angstrom resolution structure of b-galactosidase with a 200 kV cryoARM electron microscope [Escherichia coli K-12],6X1Q_D 1.8 Angstrom resolution structure of b-galactosidase with a 200 kV cryoARM electron microscope [Escherichia coli K-12]
1F4A_A	1.94e-52	1	252	370	620	E.COLI (LACZ) BETA-GALACTOSIDASE (NCS CONSTRAINED MONOMER-ORTHORHOMBIC) [Escherichia coli],1F4A_B E. COLI (LACZ) BETA-GALACTOSIDASE (NCS CONSTRAINED MONOMER-ORTHORHOMBIC) [Escherichia coli],1F4A_C E. COLI (LACZ) BETA-GALACTOSIDASE (NCS CONSTRAINED MONOMER-ORTHORHOMBIC) [Escherichia coli],1F4A_D E. COLI (LACZ) BETA-GALACTOSIDASE (NCS CONSTRAINED MONOMER-ORTHORHOMBIC) [Escherichia coli],1F4H_A E. COLI (LACZ) BETA-GALACTOSIDASE (ORTHORHOMBIC) [Escherichia coli],1F4H_B E. COLI (LACZ) BETA-GALACTOSIDASE (ORTHORHOMBIC) [Escherichia coli],1F4H_C E. COLI (LACZ) BETA-GALACTOSIDASE (ORTHORHOMBIC) [Escherichia coli],1F4H_D E. COLI (LACZ) BETA-GALACTOSIDASE (ORTHORHOMBIC) [Escherichia coli]
5A1A_A	1.94e-52	1	252	371	621	2.2A resolution cryo-EM structure of beta-galactosidase in complex with a cell-permeant inhibitor [Escherichia coli K-12],5A1A_B 2.2 A resolution cryo-EM structure of beta-galactosidase in complex with a cell-permeant inhibitor [Escherichia coli K-12],5A1A_C 2.2 A resolution cryo-EM structure of beta-galactosidase in complex with a cell-permeant inhibitor [Escherichia coli K-12],5A1A_D 2.2 A resolution cryo-EM structure of beta-galactosidase in complex with a cell-permeant inhibitor [Escherichia coli K-12]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
O07684	5.29e-136	1	258	371	628	Beta-galactosidase large subunit OS=Lactobacillus acidophilus (strain ATCC 700396 / NCK56 / N2 / NCFM) OX=272621 GN=lacL PE=3 SV=2
Q48846	2.75e-135	4	258	371	625	Beta-galactosidase large subunit OS=Latilactobacillus sakei OX=1599 GN=lacL PE=3 SV=1
Q7WTB4	1.70e-134	1	258	371	628	Beta-galactosidase large subunit OS=Lactobacillus helveticus OX=1587 GN=lacL PE=2 SV=1
Q02603	1.47e-132	1	258	369	626	Beta-galactosidase large subunit OS=Leuconostoc lactis OX=1246 GN=lacL PE=1 SV=1
P24131	5.35e-103	4	254	365	612	Beta-galactosidase OS=Clostridium acetobutylicum OX=1488 GN=cbgA PE=2 SV=2

SignalP and Lipop Annotations

This protein is predicted as OTHER

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
1.000087	0.000000	0.000000	0.000000	0.000000	0.000000

TMHMM  Annotations     

There is no transmembrane helices in MGYG000000361_01557.