CAZyme Information: MGYG000000362_00508

Basic Information

• Species: CAG-115 sp003531585
• Lineage: Bacteria; Firmicutes_A; Clostridia; Oscillospirales; Ruminococcaceae; CAG-115; CAG-115 sp003531585
• CAZyme ID: MGYG000000362_00508
• CAZy Family: GH2
• CAZyme Description: Beta-galactosidase

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
1133	MGYG000000362_4|CGC1	125852.6	4.3965

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000000362	2638970	MAG	Sweden	Europe

• Gene Location: Start: 110423; End: 113824 Strand: -

Full Sequence      

MTAQNILFNDGWQFCLCDIGTELSALPGRHWYDVELPHDWLINDTSKLYETGEGWYRRSL
PCSAEQLSGRVLLNFDGVYMNSTLFVNGKEAGSWTYGYSAFEHDITDFLHEGENELLLRV
SHQSPNTRWYSGAGIFRDVMLKLRPAAYIGTNGVYIHSAPQPEGGWTTEVETDVVGEASD
IRMLLEVFDPAGASMGGYGLEAHFDGGHEKFTASFNSTDPELWSVDDPMLYTLKISLYSG
SELLDCVNVTFGYRTAEFDPDRGFLLNGEPVKLHGVCMHHDLGALGSALNEAALARQLRI
MKEMGVNAIRTSHNMPARQLVQLCDEMGLLVDSEAFDMWEKPKTEFDNHRFFTEHAERDV
RSWIERDRNHPCIIMWSIGNEINDTIDPHGLDITKRLYEYVLKYDPKGNAAPTIGSNYMG
NENAQKCSDVVKLAGYNYSEYLYDEHHAKYPDWVIYGSETASAVRSRGIYRFPAELPLLT
GEDCQCSSLDNSVVGWGSSAMKSWRLDRDCPFCCGQFIWTGFDYIGEPTPYNTKNSYFGI
VDTAGFPKDIYYFYQSVWLSPEQKKVLHIVPSYWDFIPGQEIDVLIYSNARDVELFLNGK
SIGSHVMELETSQDMRAHFKVPFEPGVLRVVGHFADGSECSEVLHTPSDPAAVVMTSDKE
TLLADGRDIAFVEISTVDVNGIPVGNARNLIRVEVSGAGRLVGLDNGDSTDYDSYKGDNR
RLFSGKLLAMIESTLEPGEITVRAYSEGLENAELRLVSENCGEVSGVSVVTENKFPAVCR
AYTGEVPARLLLPEVDVNSFDPERRSAEIRAKLLPENCTYDDISWSVVRRNGVESSLAQV
EGSGRSAVVTAKGDGEFFVRAMVHNGAEHPQVIADIPFTAEGLGAAVRDAYSFISASTLD
SSNVPTNIIEDGALSNFDGRTVMTYSDIDFGKTGSQIISLSVGTCFDMPVEVWEGTPDDG
KLICRVDFGNNGHWCGFAGQDFALAERLTGVRTISVVIDSRVIFGGFSFVPIERAYDTNW
VGEADSVYGDDYRIDGRRVADIGNNVIINYEGLDFGEGGSEALIISGETGNPMNQIQLRY
TPAGGAQKTVLLEFQQDGGREQRFDIPKLSGVNDVSFVFMPGSRFDFDWFRFE

Enzyme Prediction     

EC	3.2.1.23

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GH2	3	627	1.1e-117	0.7007978723404256

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
COG3250	LacZ	4.54e-71	1	558	8	595	Beta-galactosidase/beta-glucuronidase [Carbohydrate transport and metabolism].
PRK10150	PRK10150	6.06e-42	52	381	65	417	beta-D-glucuronidase; Provisional
pfam18565	Glyco_hydro2_C5	4.05e-39	654	755	2	103	Glycoside hydrolase family 2 C-terminal domain 5. Domain 5 is found in dimeric beta-D-galactosidase from Paracoccus sp. 32d, which contributes to stabilization of the functional dimer. It is suggested that the location of this domain 5, may be one of the factors responsible for the creation of a functional dimer and cold-adaptation of this enzyme.
PRK10340	ebgA	3.04e-38	31	381	71	449	cryptic beta-D-galactosidase subunit alpha; Reviewed
PRK09525	lacZ	2.58e-34	54	381	122	462	beta-galactosidase.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
BCN28859.1	0.0	7	1132	8	1153
CBL35301.1	0.0	1	1132	1	1146
QYR22526.1	0.0	4	1132	3	1144
AGA58497.1	0.0	6	1133	5	1140
AZK49101.1	0.0	4	1133	3	1148

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
7CWD_A	1.47e-188	4	756	2	799	ChainA, beta-glalactosidase [Niallia circulans],7CWI_A Chain A, beta-galactosidase [Niallia circulans]
4YPJ_A	1.98e-179	4	751	8	800	ChainA, Beta galactosidase [Niallia circulans],4YPJ_B Chain B, Beta galactosidase [Niallia circulans]
6QUB_B	9.11e-164	8	758	12	853	Truncatedbeta-galactosidase III from Bifidobacterium bifidum in complex with galactose [Bifidobacterium bifidum]
6QUB_A	2.02e-163	8	758	35	876	Truncatedbeta-galactosidase III from Bifidobacterium bifidum in complex with galactose [Bifidobacterium bifidum],6QUC_A Truncated beta-galactosidase III from Bifidobacterium bifidum [Bifidobacterium bifidum],6QUC_B Truncated beta-galactosidase III from Bifidobacterium bifidum [Bifidobacterium bifidum],6QUD_A 2-deoxy-galactose reaction intermediate of a Truncated beta-galactosidase III from Bifidobacterium bifidum [Bifidobacterium bifidum]
5DMY_A	3.44e-163	8	758	41	882	Beta-galactosidase- construct 33-930 [Bifidobacterium bifidum],5DMY_B Beta-galactosidase - construct 33-930 [Bifidobacterium bifidum],5DMY_C Beta-galactosidase - construct 33-930 [Bifidobacterium bifidum]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
A7LXS9	1.23e-166	8	759	47	849	Beta-galactosidase BoGH2A OS=Bacteroides ovatus (strain ATCC 8483 / DSM 1896 / JCM 5824 / BCRC 10623 / CCUG 4943 / NCTC 11153) OX=411476 GN=BACOVA_02645 PE=1 SV=1
T2KM09	1.45e-99	8	742	51	804	Putative beta-glucuronidase OS=Formosa agariphila (strain DSM 15362 / KCTC 12365 / LMG 23005 / KMM 3901 / M-2Alg 35-1) OX=1347342 GN=BN863_22050 PE=2 SV=2
P77989	4.56e-55	9	746	20	725	Beta-galactosidase OS=Thermoanaerobacter pseudethanolicus (strain ATCC 33223 / 39E) OX=340099 GN=lacZ PE=3 SV=2
T2KPJ7	6.89e-54	2	626	52	696	Putative beta-glucuronidase OS=Formosa agariphila (strain DSM 15362 / KCTC 12365 / LMG 23005 / KMM 3901 / M-2Alg 35-1) OX=1347342 GN=BN863_21970 PE=2 SV=1
T2KN75	2.44e-42	3	758	45	795	Beta-glucuronidase OS=Formosa agariphila (strain DSM 15362 / KCTC 12365 / LMG 23005 / KMM 3901 / M-2Alg 35-1) OX=1347342 GN=BN863_22060 PE=1 SV=1

SignalP and Lipop Annotations

This protein is predicted as OTHER

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
1.000055	0.000000	0.000000	0.000000	0.000000	0.000000

TMHMM  Annotations     

There is no transmembrane helices in MGYG000000362_00508.