CAZyme Information: MGYG000000393_01370

Basic Information

• Lineage: Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; UBA932; RC9
• CAZyme ID: MGYG000000393_01370
• CAZy Family: GH127
• CAZyme Description: hypothetical protein

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
621		69396.22	6.0027

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000000393	2242585	MAG	Sweden	Europe

• Gene Location: Start: 1733; End: 3598 Strand: +

Full Sequence      

MKYRFLTSTALLLGALSSVATATERKPAPEIRNVKIEGYAGDRIDKCIANITMPVDIDRL
VQPFRAQDETQNRWQTEFWGKWVQGAIDSYLYNADTVLYEKIGKSVALMAETQLPDGYIG
NYDKEHQLDGWDVWGRKYTTLGLLKWHGLSGDRKALDAACRLIDYTITQLGPGAVPVYAT
GCYKGMASGSILEPVVMLYNATGNGKYLDFARHLACSLDEAGGPQLISLADTRLDRRFPV
GPDMWWSNDNGQKAYEMMSCYIGFLELYKVTGETRYLDAVEKVVKRIMDEEINVCGSAAA
SECWFGGKLRQTEPNVDAMETCVTFTWMQLNERLLQITGKPEYAGNIEKSMFNALFASMK
HDGSEIVKYLPLEGFRMGADGQCGMPLHCCNANAPRAFAMIPRFAWTVRDSAVKINLFTP
SSAEVKVGKNEARLTMATGYPLDGNSEITVECRKKMSFALRVRIPEWAENAEVKVNGSPL
GCVTAGEYAVIDRTWNNGDKVTVNFEMTPRLVMLDNHQAIVRGPVTLARDSRFNDGFVDE
ACIIPAGSDGKVELRPVAAPDNMWMAYEITVAVGTYGNEGRMPSRTIHFCDFASAGNDWD
FNGRYRVWLPRSVEVRFQPRR

Enzyme Prediction     

No EC number prediction in MGYG000000393_01370.

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GH127	72	527	9.3e-58	0.8893129770992366

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
pfam07944	Glyco_hydro_127	4.07e-64	33	528	1	503	Beta-L-arabinofuranosidase, GH127. One member of this family, from Bidobacterium longicum, UniProtKB:E8MGH8, has been characterized as an unusual beta-L-arabinofuranosidase enzyme, EC:3.2.1.185. It rleases l-arabinose from the l-arabinofuranose (Araf)-beta1,2-Araf disaccharide and also transglycosylates 1-alkanols with retention of the anomeric configuration. Terminal beta-l-arabinofuranosyl residues have been found in arabinogalactan proteins from a mumber of different plantt species. beta-l-Arabinofuranosyl linkages with 1-4 arabinofuranosides are also found in the sugar chains of extensin and solanaceous lectins, hydroxyproline (Hyp)2-rich glycoproteins that are widely observed in plant cell wall fractions. The critical residue for catalytic activity is Glu-338, in a ET/SCAS sequence context.
COG3533	COG3533	5.88e-54	26	537	4	509	Uncharacterized conserved protein, DUF1680 family [Function unknown].
COG1331	YyaL	0.001	142	287	419	567	Uncharacterized conserved protein YyaL, SSP411 family, contains thoiredoxin and six-hairpin glycosidase-like domains [General function prediction only].
COG4225	YesR	0.004	129	278	32	158	Rhamnogalacturonyl hydrolase YesR [Carbohydrate transport and metabolism].

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
ARE60508.1	9.27e-185	31	619	24	616
QIX65272.1	2.33e-42	2	610	3	615
QUR48577.1	3.17e-42	2	610	3	615
BBK93301.1	3.17e-42	2	610	3	615
QJE30089.1	4.33e-42	2	610	3	615

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
6EX6_A	1.27e-35	81	534	74	554	TheGH127, Beta-arabinofuranosidase, BT3674 [Bacteroides thetaiotaomicron VPI-5482],6EX6_B The GH127, Beta-arabinofuranosidase, BT3674 [Bacteroides thetaiotaomicron VPI-5482]
5MQO_A	1.48e-27	81	531	105	610	Glycosidehydrolase BT_1003 [Bacteroides thetaiotaomicron]
3WRE_A	9.37e-19	81	536	80	574	Thecrystal structure of native HypBA1 from Bifidobacterium longum JCM 1217 [Bifidobacterium longum subsp. longum JCM 1217],3WRG_A The complex structure of HypBA1 with L-arabinose [Bifidobacterium longum subsp. longum JCM 1217]
3WKW_A	9.56e-19	81	536	80	574	Crystalstructure of GH127 beta-L-arabinofuranosidase HypBA1 from Bifidobacterium longum ligand free form [Bifidobacterium longum subsp. longum JCM 1217],3WKX_A Crystal structure of GH127 beta-L-arabinofuranosidase HypBA1 from Bifidobacterium longum arabinose complex form [Bifidobacterium longum subsp. longum JCM 1217],7BZL_A Chain A, Non-reducing end beta-L-arabinofuranosidase [Bifidobacterium longum subsp. longum JCM 1217],7DIF_A Chain A, Non-reducing end beta-L-arabinofuranosidase [Bifidobacterium longum subsp. longum JCM 1217],7EXV_A Chain A, Non-reducing end beta-L-arabinofuranosidase [Bifidobacterium longum subsp. longum JCM 1217],7EXW_A Chain A, Non-reducing end beta-L-arabinofuranosidase [Bifidobacterium longum subsp. longum JCM 1217]
7EXU_A	9.56e-19	81	536	80	574	ChainA, Non-reducing end beta-L-arabinofuranosidase [Bifidobacterium longum subsp. longum JCM 1217]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
E8MGH8	5.13e-18	81	536	80	574	Non-reducing end beta-L-arabinofuranosidase OS=Bifidobacterium longum subsp. longum (strain ATCC 15707 / DSM 20219 / JCM 1217 / NCTC 11818 / E194b) OX=565042 GN=hypBA1 PE=1 SV=1

SignalP and Lipop Annotations

This protein is predicted as SP

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
0.000248	0.999141	0.000175	0.000157	0.000142	0.000125

TMHMM  Annotations     

There is no transmembrane helices in MGYG000000393_01370.