CAZyme Information: MGYG000000405_01708

Basic Information

• Species: CAG-390 sp000437015
• Lineage: Bacteria; Firmicutes_A; Clostridia; Oscillospirales; CAG-272; CAG-390; CAG-390 sp000437015
• CAZyme ID: MGYG000000405_01708
• CAZy Family: GT8
• CAZyme Description: hypothetical protein

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
285		32924.14	9.2924

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000000405	2251507	MAG	Sweden	Europe

• Gene Location: Start: 68402; End: 69259 Strand: -

Full Sequence      

MNILYCGDKNIADGLTLSVLSLIGHTREPLNILILTADLAAGNRRCVALPEDFAEFLDMR
LKVFDPRHSVRLFNVTPLFGAEPPTANLGTRFTPCCMLRLYADLVPDVPDRVLYLDNDVV
CRGDFSELYYTDLGGAELAGVPDYYGRWFFRRRVIVHDYLNSGVLLLDMKKIRADGLFRR
CRRMCAAKRMFMPDQSALNKLAKSKKIMPRRYNEQRRQQNNTVFRHFTTTFRLFPTFHTV
SVKPWQEDKMHEVLKIYEYDALIAESGRLMADYRAFRDRSGGISS

Enzyme Prediction     

No EC number prediction in MGYG000000405_01708.

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GT8	2	248	2.6e-34	0.8754863813229572

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
cd04194	GT8_A4GalT_like	2.96e-49	1	246	1	247	A4GalT_like proteins catalyze the addition of galactose or glucose residues to the lipooligosaccharide (LOS) or lipopolysaccharide (LPS) of the bacterial cell surface. The members of this family of glycosyltransferases catalyze the addition of galactose or glucose residues to the lipooligosaccharide (LOS) or lipopolysaccharide (LPS) of the bacterial cell surface. The enzymes exhibit broad substrate specificities. The known functions found in this family include: Alpha-1,4-galactosyltransferase, LOS-alpha-1,3-D-galactosyltransferase, UDP-glucose:(galactosyl) LPS alpha1,2-glucosyltransferase, UDP-galactose: (glucosyl) LPS alpha1,2-galactosyltransferase, and UDP-glucose:(glucosyl) LPS alpha1,2-glucosyltransferase. Alpha-1,4-galactosyltransferase from N. meningitidis adds an alpha-galactose from UDP-Gal (the donor) to a terminal lactose (the acceptor) of the LOS structure of outer membrane. LOSs are virulence factors that enable the organism to evade the immune system of host cells. In E. coli, the three alpha-1,2-glycosyltransferases, that are involved in the synthesis of the outer core region of the LPS, are all members of this family. The three enzymes share 40 % of sequence identity, but have different sugar donor or acceptor specificities, representing the structural diversity of LPS.
COG1442	RfaJ	4.80e-26	1	251	3	250	Lipopolysaccharide biosynthesis protein, LPS:glycosyltransferase [Cell wall/membrane/envelope biogenesis].
pfam01501	Glyco_transf_8	1.05e-25	2	245	1	248	Glycosyl transferase family 8. This family includes enzymes that transfer sugar residues to donor molecules. Members of this family are involved in lipopolysaccharide biosynthesis and glycogen synthesis. This family includes Lipopolysaccharide galactosyltransferase, lipopolysaccharide glucosyltransferase 1, and glycogenin glucosyltransferase.
cd00505	Glyco_transf_8	4.32e-17	5	213	6	212	Members of glycosyltransferase family 8 (GT-8) are involved in lipopolysaccharide biosynthesis and glycogen synthesis. Members of this family are involved in lipopolysaccharide biosynthesis and glycogen synthesis. GT-8 comprises enzymes with a number of known activities: lipopolysaccharide galactosyltransferase, lipopolysaccharide glucosyltransferase 1, glycogenin glucosyltransferase, and N-acetylglucosaminyltransferase. GT-8 enzymes contains a conserved DXD motif which is essential in the coordination of a catalytic divalent cation, most commonly Mn2+.
PRK15171	PRK15171	2.85e-10	1	247	26	272	lipopolysaccharide 3-alpha-galactosyltransferase.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
ANZ62930.1	1.45e-104	1	265	1	265
ANZ65909.1	1.45e-104	1	265	1	265
ANZ60115.1	1.45e-104	1	265	1	265
QFQ91722.1	1.33e-101	1	269	2	266
AMV65062.1	9.03e-101	1	265	1	265

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
1SS9_A	1.38e-07	1	179	1	171	ChainA, alpha-1,4-galactosyl transferase [Neisseria meningitidis]
1G9R_A	1.43e-06	2	179	2	171	ChainA, GLYCOSYL TRANSFERASE [Neisseria meningitidis],1GA8_A Chain A, GALACTOSYL TRANSFERASE LGTC [Neisseria meningitidis]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
P27129	9.32e-10	99	258	112	285	Lipopolysaccharide 1,2-glucosyltransferase OS=Escherichia coli (strain K12) OX=83333 GN=rfaJ PE=3 SV=2
P19817	1.89e-07	1	213	26	233	Lipopolysaccharide 1,2-glucosyltransferase OS=Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720) OX=99287 GN=rfaJ PE=3 SV=4
Q9M8J2	6.37e-07	8	173	73	243	Probable galacturonosyltransferase-like 4 OS=Arabidopsis thaliana OX=3702 GN=GATL4 PE=2 SV=1
P19816	8.47e-06	100	215	114	241	Lipopolysaccharide 1,3-galactosyltransferase OS=Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720) OX=99287 GN=rfaI PE=3 SV=4

SignalP and Lipop Annotations

This protein is predicted as OTHER

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
1.000055	0.000000	0.000000	0.000000	0.000000	0.000000

TMHMM  Annotations     

There is no transmembrane helices in MGYG000000405_01708.