CAZyme Information: MGYG000000418_00289

Basic Information

• Species: UBA1820 sp003150615
• Lineage: Bacteria; Bacteroidota; Bacteroidia; Flavobacteriales; UBA1820; UBA1820; UBA1820 sp003150615
• CAZyme ID: MGYG000000418_00289
• CAZy Family: GH20
• CAZyme Description: N,N'-diacetylchitobiase

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
851	MGYG000000418_1|CGC3	94947.8	5.503

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000000418	2050770	MAG	Sweden	Europe

• Gene Location: Start: 325824; End: 328379 Strand: +

Full Sequence      

MLKQLLLSALIIPALAACSSGKKAAEVAPVEVSWAMQGNQKGPNNIYYQTEFCFRNTSDK
PLDNNWAIYYNQFPRVPLHSDSSAVKAQQIVGNFYRIYPTEYYKPIAPGDSLKVTILFRG
SSIKEIEAPMGMYFVPCDADGQELTPMKMAPVKVAPYGNDIHKRNSGDNYPYPTGQFLYA
QDQGIVLGQPLKDYDIIPSVKSAVPGQDTVVIGKKISVSAPEELKNEADFLSGKLKKDYG
AEVGTSEGAYPVKLALDPSLKAKNDEAYAVSLAKDGAVITGATPAAVLLGVQTLRGIIGV
TQLPVSLQSVAIEDQPDFAYRGFMLDIARNFQTKETIEKVLDQMSYYKLNKFHFHFNDDE
SWRLEIPGLPELTEYGARRGHTLDEKNMLHPTYGSGPFADDPTSRGYGYFTREQFIDLLR
YATKLHIDVIPEIETPGHARAAVYSMKARYEKYKDSDMDKAMEYLMSDDADTSKYTSAQG
FHDNVMCVAREGVYNFLDKVVTEIQAMYKEAGAPLYAIQTGGDEVPRGSWLGSPVCQALV
ESGKVESLGDLRDYFTERFKKILDDKGLKYYGWMEIASKKGKPNPKFQNAGFTAYCWDTV
GEWGGEQVPYILANAGIDIILCNAPNLYLDFAYNKHPQEPGLYWGGWVNERNTYDLLPYD
VYRSVRMGMRGDVMDWDKANHKPDGSLKEQLTAEGRKHIRGLQAELWHETVKSEDMMEYY
MFPKILGMAERAWNAVPHWSLVKDAKQRDAEYGLGLGEYLTKIAVKEMPYFQREGLNFRV
ASPGIEIKDGMLYISNSVPGAAVHYTTDGSTPDEKSPVWTAPVAVAGTNVKAVTTAFGKK
STVSEYILPAE

Enzyme Prediction     

No EC number prediction in MGYG000000418_00289.

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GH20	314	735	6.1e-93	0.9703264094955489

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
cd06569	GH20_Sm-chitobiase-like	0.0	314	752	1	434	The chitobiase of Serratia marcescens is a beta-N-1,4-acetylhexosaminidase with a glycosyl hydrolase family 20 (GH20) domain that hydrolyzes the beta-1,4-glycosidic linkages in oligomers derived from chitin. Chitin is degraded by a two step process: i) a chitinase hydrolyzes the chitin to oligosaccharides and disaccharides such as di-N-acetyl-D-glucosamine and chitobiose, ii) chitobiase then further degrades these oligomers into monomers. The GH20 hexosaminidases are thought to act via a catalytic mechanism in which the catalytic nucleophile is not provided by solvent or the enzyme, but by the substrate itself.
cd06563	GH20_chitobiase-like	2.81e-105	318	733	1	342	The chitobiase of Serratia marcescens is a beta-N-1,4-acetylhexosaminidase with a glycosyl hydrolase family 20 (GH20) domain that hydrolyzes the beta-1,4-glycosidic linkages in oligomers derived from chitin. Chitin is degraded by a two step process: i) a chitinase hydrolyzes the chitin to oligosaccharides and disaccharides such as di-N-acetyl-D-glucosamine and chitobiose, ii) chitobiase then further degrades these oligomers into monomers. This GH20 domain family includes an N-acetylglucosamidase (GlcNAcase A) from Pseudoalteromonas piscicida and an N-acetylhexosaminidase (SpHex) from Streptomyces plicatus. SpHex lacks the C-terminal PKD (polycystic kidney disease I)-like domain found in the chitobiases. The GH20 hexosaminidases are thought to act via a catalytic mechanism in which the catalytic nucleophile is not provided by solvent or the enzyme, but by the substrate itself.
pfam00728	Glyco_hydro_20	1.11e-97	318	735	1	345	Glycosyl hydrolase family 20, catalytic domain. This domain has a TIM barrel fold.
COG3525	Chb	1.67e-86	194	834	134	701	N-acetyl-beta-hexosaminidase [Carbohydrate transport and metabolism].
cd06568	GH20_SpHex_like	1.65e-57	318	740	1	323	A subgroup of the Glycosyl hydrolase family 20 (GH20) catalytic domain found in proteins similar to the N-acetylhexosaminidase from Streptomyces plicatus (SpHex). SpHex catalyzes the hydrolysis of N-acetyl-beta-hexosaminides. An Asp residue within the active site plays a critical role in substrate-assisted catalysis by orienting the 2-acetamido group and stabilizing the transition state. The GH20 hexosaminidases are thought to act via a catalytic mechanism in which the catalytic nucleophile is not provided by solvent or the enzyme, but by the substrate itself. Proteins belonging to this subgroup lack the C-terminal PKD (polycystic kidney disease I)-like domain found in the chitobiases.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
VTZ55501.1	2.15e-226	28	841	15	811
QUT34090.1	3.51e-226	28	841	30	826
BBK89309.1	4.31e-226	28	841	15	811
AHF12728.1	1.50e-225	6	849	9	834
QUU00208.1	2.80e-225	28	841	30	826

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
1QBA_A	2.85e-103	65	833	57	845	BACTERIALCHITOBIASE, GLYCOSYL HYDROLASE FAMILY 20 [Serratia marcescens],1QBB_A BACTERIAL CHITOBIASE COMPLEXED WITH CHITOBIOSE (DINAG) [Serratia marcescens]
1C7T_A	1.49e-102	65	833	57	845	ChainA, BETA-N-ACETYLHEXOSAMINIDASE [Serratia marcescens]
1C7S_A	7.79e-102	65	833	57	845	ChainA, BETA-N-ACETYLHEXOSAMINIDASE [Serratia marcescens]
6EZR_A	2.84e-63	311	779	256	638	Crystalstructure of GH20 Exo beta-N-Acetylglucosaminidase from Vibrio harveyi [Vibrio harveyi],6EZR_B Crystal structure of GH20 Exo beta-N-Acetylglucosaminidase from Vibrio harveyi [Vibrio harveyi],6EZS_A Crystal structure of GH20 Exo beta-N-Acetylglucosaminidase from Vibrio harveyi in complex with N-acetylglucosamine [Vibrio harveyi],6EZS_B Crystal structure of GH20 Exo beta-N-Acetylglucosaminidase from Vibrio harveyi in complex with N-acetylglucosamine [Vibrio harveyi],6K35_A Crystal structure of GH20 exo beta-N-acetylglucosaminidase from Vibrio harveyi in complex with NAG-thiazoline [Vibrio harveyi],6K35_B Crystal structure of GH20 exo beta-N-acetylglucosaminidase from Vibrio harveyi in complex with NAG-thiazoline [Vibrio harveyi]
6EZT_A	3.37e-62	311	779	253	635	Crystalstructure of GH20 Exo beta-N-Acetylglucosaminidase D437A inactive mutant from Vibrio harveyi [Vibrio harveyi],6EZT_B Crystal structure of GH20 Exo beta-N-Acetylglucosaminidase D437A inactive mutant from Vibrio harveyi [Vibrio harveyi]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
Q04786	1.10e-107	41	811	34	811	Beta-hexosaminidase OS=Vibrio vulnificus OX=672 GN=hex PE=3 SV=1
P13670	2.57e-104	65	834	82	880	N,N'-diacetylchitobiase OS=Vibrio harveyi OX=669 GN=chb PE=1 SV=1
Q54468	1.03e-101	65	833	84	872	Chitobiase OS=Serratia marcescens OX=615 GN=chb PE=1 SV=1
P49007	1.24e-80	165	773	183	762	Beta-hexosaminidase B OS=Pseudoalteromonas piscicida OX=43662 GN=nag096 PE=3 SV=1
P96155	6.59e-62	310	729	252	601	Beta-hexosaminidase OS=Vibrio furnissii OX=29494 GN=exoI PE=1 SV=1

SignalP and Lipop Annotations

This protein is predicted as LIPO

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
0.000003	0.001514	0.998521	0.000001	0.000001	0.000001

TMHMM  Annotations     

There is no transmembrane helices in MGYG000000418_00289.