CAZyme Information: MGYG000000440_02091

Basic Information

• Lineage: Bacteria; Firmicutes_A; Clostridia; Oscillospirales; CAG-272; UMGS1865
• CAZyme ID: MGYG000000440_02091
• CAZy Family: GH2
• CAZyme Description: Beta-galactosidase

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
587	MGYG000000440_21|CGC1	65825.71	5.1383

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000000440	2422037	MAG	Sweden	Europe

• Gene Location: Start: 8472; End: 10235 Strand: -

Full Sequence      

MTQVPRGEYPRPQLVRENWQCLNGTWEFEIDAGESGEARGLPTAERLGGKITVPFCPESR
LSGVAHTDFMRCVWYARDLDIPAAWKGKRILLHFGAVDYLATVWINGKKVGTHAGGYTPF
SFDITDFLNFEHDRVTLSAFDDVRSHNQPAGKQSSRYASHGCSYTRTTGIWQTVWMEAVE
DLHIKSLHTTPDTAACAVTTECRLAGDPMGADLSVRVTFGGKEVGTATAKGITGNSVTVT
VPLSEEHLWDVGVGNLYDVEYILARGGKTVDRVTSYFGLRSVALVGRTFRVNGRVVFGRW
VLDQGFYPDGIYTAPTDEDLKNDILKSMELGFNGARLHEKVFEPRFLLWADRLGYLCWGE
QANWGLDITEAGAIANFLPEWLEAVERDRNHPSVIGWCPFNETWDFCGRRQADSVLAQVY
RVTKALDATRPVIDTSGNFHVETDIFDVHDYEQDPAKFAACYAQIDAGIVNDQCERSADR
SRSGRQHYDGKSPVFVSEYGGIRWTTEDGWGYGKGPETEEEFLARYKGLTDALLDNANIM
GFCYTQLYDVEQEQNGLMTYGREFKFDPAVIRAINTRAAAIEKKYNR

Enzyme Prediction     

No EC number prediction in MGYG000000440_02091.

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GH2	15	512	1.8e-99	0.5837765957446809

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
COG3250	LacZ	1.34e-45	22	567	15	556	Beta-galactosidase/beta-glucuronidase [Carbohydrate transport and metabolism].
PRK10150	PRK10150	6.46e-39	9	432	3	445	beta-D-glucuronidase; Provisional
PRK09525	lacZ	6.30e-15	75	432	124	485	beta-galactosidase.
PRK10340	ebgA	6.66e-15	51	446	99	486	cryptic beta-D-galactosidase subunit alpha; Reviewed
pfam02837	Glyco_hydro_2_N	1.05e-14	22	140	4	134	Glycosyl hydrolases family 2, sugar binding domain. This family contains beta-galactosidase, beta-mannosidase and beta-glucuronidase activities and has a jelly-roll fold. The domain binds the sugar moiety during the sugar-hydrolysis reaction.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
ALS26546.1	1.58e-250	4	584	9	588
QOS82722.1	1.05e-246	1	582	1	579
QLG42947.1	2.56e-242	1	582	1	578
ANY74929.1	5.73e-242	2	582	5	581
AWP26385.1	6.16e-241	2	582	4	579

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
7SF2_A	7.47e-92	8	584	28	588	ChainA, Glycosyl hydrolase family 2, sugar binding domain protein [Bacteroides cellulosilyticus DSM 14838],7SF2_B Chain B, Glycosyl hydrolase family 2, sugar binding domain protein [Bacteroides cellulosilyticus DSM 14838],7SF2_C Chain C, Glycosyl hydrolase family 2, sugar binding domain protein [Bacteroides cellulosilyticus DSM 14838],7SF2_D Chain D, Glycosyl hydrolase family 2, sugar binding domain protein [Bacteroides cellulosilyticus DSM 14838],7SF2_E Chain E, Glycosyl hydrolase family 2, sugar binding domain protein [Bacteroides cellulosilyticus DSM 14838],7SF2_F Chain F, Glycosyl hydrolase family 2, sugar binding domain protein [Bacteroides cellulosilyticus DSM 14838]
3K4A_A	1.31e-34	22	448	17	464	Crystalstructure of selenomethionine substituted E. coli beta-glucuronidase [Escherichia coli K-12],3K4A_B Crystal structure of selenomethionine substituted E. coli beta-glucuronidase [Escherichia coli K-12]
4JHZ_A	2.36e-34	22	448	17	464	Structureof E. coli beta-Glucuronidase bound with a novel, potent inhibitor 2-[4-(1,3-benzodioxol-5-ylmethyl)piperazin-1-yl]-N-[(1S,2S,5S)-2,5-dimethoxycyclohexyl]acetamide [Escherichia coli K-12],4JHZ_B Structure of E. coli beta-Glucuronidase bound with a novel, potent inhibitor 2-[4-(1,3-benzodioxol-5-ylmethyl)piperazin-1-yl]-N-[(1S,2S,5S)-2,5-dimethoxycyclohexyl]acetamide [Escherichia coli K-12]
3LPF_A	2.40e-34	22	448	17	464	Structureof E. coli beta-Glucuronidase bound with a novel, potent inhibitor 1-((6,7-dimethyl-2-oxo-1,2-dihydroquinolin-3-yl)methyl)-1-(2-hydroxyethyl)-3-(3-methoxyphenyl)thiourea [Escherichia coli K-12],3LPF_B Structure of E. coli beta-Glucuronidase bound with a novel, potent inhibitor 1-((6,7-dimethyl-2-oxo-1,2-dihydroquinolin-3-yl)methyl)-1-(2-hydroxyethyl)-3-(3-methoxyphenyl)thiourea [Escherichia coli K-12],3LPG_A Structure of E. coli beta-Glucuronidase bound with a novel, potent inhibitor 3-(2-fluorophenyl)-1-(2-hydroxyethyl)-1-((6-methyl-2-oxo-1,2-dihydroquinolin-3-yl)methyl)urea [Escherichia coli K-12],3LPG_B Structure of E. coli beta-Glucuronidase bound with a novel, potent inhibitor 3-(2-fluorophenyl)-1-(2-hydroxyethyl)-1-((6-methyl-2-oxo-1,2-dihydroquinolin-3-yl)methyl)urea [Escherichia coli K-12],5CZK_A Structure of E. coli beta-glucuronidase bound with a novel, potent inhibitor 1-((6,8-dimethyl-2-oxo-1,2-dihydroquinolin-3-yl)methyl)-1-(2-hydroxyethyl)-3-(4-hydroxyphenyl)thiourea [Escherichia coli K-12],5CZK_B Structure of E. coli beta-glucuronidase bound with a novel, potent inhibitor 1-((6,8-dimethyl-2-oxo-1,2-dihydroquinolin-3-yl)methyl)-1-(2-hydroxyethyl)-3-(4-hydroxyphenyl)thiourea [Escherichia coli K-12]
6LEM_B	7.71e-34	22	448	13	460	ChainB, Beta-D-glucuronidase [Escherichia coli]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
P05804	4.29e-33	22	448	15	462	Beta-glucuronidase OS=Escherichia coli (strain K12) OX=83333 GN=uidA PE=1 SV=2
P77989	7.05e-27	22	431	19	411	Beta-galactosidase OS=Thermoanaerobacter pseudethanolicus (strain ATCC 33223 / 39E) OX=340099 GN=lacZ PE=3 SV=2
O97524	7.39e-26	9	451	29	503	Beta-glucuronidase OS=Felis catus OX=9685 GN=GUSB PE=1 SV=1
O18835	1.31e-25	9	451	29	503	Beta-glucuronidase OS=Canis lupus familiaris OX=9615 GN=GUSB PE=1 SV=1
Q4FAT7	2.27e-23	9	432	30	479	Beta-glucuronidase OS=Sus scrofa OX=9823 GN=GUSB PE=3 SV=1

SignalP and Lipop Annotations

This protein is predicted as OTHER

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
1.000087	0.000000	0.000000	0.000000	0.000000	0.000000

TMHMM  Annotations     

There is no transmembrane helices in MGYG000000440_02091.