CAZyme Information: MGYG000000442_00406

Basic Information

• Species: CAG-462 sp003489705
• Lineage: Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Bacteroidaceae; CAG-462; CAG-462 sp003489705
• CAZyme ID: MGYG000000442_00406
• CAZy Family: GH127
• CAZyme Description: Non-reducing end beta-L-arabinofuranosidase

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
695	MGYG000000442_3|CGC2	78148.77	6.1362

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000000442	3125088	MAG	Sweden	Europe

• Gene Location: Start: 116475; End: 118562 Strand: +

Full Sequence      

MIKKLFSTFALAVCVSGAAVAQSGGLTDMSHSKYSKMVNTPLGAVKWTGGFWGERFDVYS
NTSLQSMWATWSNPDISHGFRNFEIAAGTCEGEHWGPPFHDGDMYKWLEAVAAVYAVNKD
PELDALMDKFIEQVVKAQRADGYIHTPVIIEERNKGIDTHSENRQQTVIGTKVGAEDEKG
AFANRLNFETYNLGHLMMAGITHYRATGKRTLFDAAIKATDFLCHFYEMASAELARNAIC
PSHYMGVVEMYRATGNPRYLELSKNLIDIRGMVENGTDDNQDRIPFREQYNAMGHAVRAN
YLYAGVADVYAETGEEQLMKNLTSIWNDIVNRKMYVTGACGALYDGTSPDGTCYEPDSIQ
KVHQSYGRAYQLPNSTAHNETCANIGNMLFNWRMLESTGDAKYADIVETALYNSVLSGVS
LDGKRYFYTNPLRLSYDFPYTLRWPRERQEYISCFCCPPNTLRTICEAQNYAYTVSENAL
WCNLYGQSELTTKLGKHDLKVSQVTGYPYDGKVVLTFNAVPKKQEMALHLRIPAWCDKAT
VTVNGEPVAVEAKANTYATINRVWKKGDVVTLDMEMRVKLMESNPLVEETRNQVVVKRGP
LVYCLEGMDVADGKRIGDVLIPADIQFTPKEITIDGSKMVALEGEARLMQEDSWDNVLYR
EVGKADQKVKIQLIPYFAWGNRGKSEMTVWMPLAK

Enzyme Prediction     

EC	3.2.1.-

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GH127	45	604	7.9e-193	0.9980916030534351

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
pfam07944	Glyco_hydro_127	0.0	44	604	1	503	Beta-L-arabinofuranosidase, GH127. One member of this family, from Bidobacterium longicum, UniProtKB:E8MGH8, has been characterized as an unusual beta-L-arabinofuranosidase enzyme, EC:3.2.1.185. It rleases l-arabinose from the l-arabinofuranose (Araf)-beta1,2-Araf disaccharide and also transglycosylates 1-alkanols with retention of the anomeric configuration. Terminal beta-l-arabinofuranosyl residues have been found in arabinogalactan proteins from a mumber of different plantt species. beta-l-Arabinofuranosyl linkages with 1-4 arabinofuranosides are also found in the sugar chains of extensin and solanaceous lectins, hydroxyproline (Hyp)2-rich glycoproteins that are widely observed in plant cell wall fractions. The critical residue for catalytic activity is Glu-338, in a ET/SCAS sequence context.
COG3533	COG3533	6.66e-135	49	691	20	585	Uncharacterized conserved protein, DUF1680 family [Function unknown].

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
QCD40914.1	0.0	6	695	5	695
AII62783.1	0.0	11	695	10	698
QEW37287.1	0.0	11	695	10	698
QQY43428.1	0.0	11	695	10	698
ALK84932.1	0.0	11	695	10	698

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
5MQO_A	0.0	3	695	2	698	Glycosidehydrolase BT_1003 [Bacteroides thetaiotaomicron]
4QJY_A	1.32e-128	51	691	22	645	Crystalstructure of native Ara127N, a GH127 beta-L-arabinofuranosidase from Geobacillus Stearothermophilus T6 [Geobacillus stearothermophilus],4QJY_B Crystal structure of native Ara127N, a GH127 beta-L-arabinofuranosidase from Geobacillus Stearothermophilus T6 [Geobacillus stearothermophilus]
4QK0_A	5.33e-124	51	691	22	645	Crystalstructure of Ara127N-Se, a GH127 beta-L-arabinofuranosidase from Geobacillus Stearothermophilus T6 [Geobacillus stearothermophilus],4QK0_B Crystal structure of Ara127N-Se, a GH127 beta-L-arabinofuranosidase from Geobacillus Stearothermophilus T6 [Geobacillus stearothermophilus],4QK0_C Crystal structure of Ara127N-Se, a GH127 beta-L-arabinofuranosidase from Geobacillus Stearothermophilus T6 [Geobacillus stearothermophilus],4QK0_D Crystal structure of Ara127N-Se, a GH127 beta-L-arabinofuranosidase from Geobacillus Stearothermophilus T6 [Geobacillus stearothermophilus]
6EX6_A	2.06e-111	41	693	11	625	TheGH127, Beta-arabinofuranosidase, BT3674 [Bacteroides thetaiotaomicron VPI-5482],6EX6_B The GH127, Beta-arabinofuranosidase, BT3674 [Bacteroides thetaiotaomicron VPI-5482]
7EXU_A	3.62e-87	48	689	6	654	ChainA, Non-reducing end beta-L-arabinofuranosidase [Bifidobacterium longum subsp. longum JCM 1217]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
E8MGH8	4.18e-86	48	689	6	654	Non-reducing end beta-L-arabinofuranosidase OS=Bifidobacterium longum subsp. longum (strain ATCC 15707 / DSM 20219 / JCM 1217 / NCTC 11818 / E194b) OX=565042 GN=hypBA1 PE=1 SV=1

SignalP and Lipop Annotations

This protein is predicted as SP

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
0.000300	0.998884	0.000213	0.000208	0.000202	0.000173

TMHMM  Annotations     

There is no transmembrane helices in MGYG000000442_00406.