CAZyme Information: MGYG000000445_01380

Basic Information

• Lineage: Bacteria; Proteobacteria; Alphaproteobacteria; RF32; CAG-239; RUG410
• CAZyme ID: MGYG000000445_01380
• CAZy Family: GT19
• CAZyme Description: Lipid-A-disaccharide synthase

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
384		43668.77	9.5005

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000000445	1525621	MAG	Sweden	Europe

• Gene Location: Start: 3297; End: 4451 Strand: -

Full Sequence      

MKYYLIAGEPSGDSLGARLIQALRRKDKNAEFYGVGGESMQAEGVKSLFNISDLAIMGLA
EVIPSIPRVLKRINETVADIQRVRPDAVITIDSWSFSARVHKKLRQLKLNIPQVHYVAPQ
VWAWKKKRARTMHKYIDLLLTLFPQEPKYFTPYNLATEFVGHPVIESEVVNGVKGDFKKR
HHIPEENRLLLVLPGSRHNEVERLLPTFLEVVKQLHERYNDFSFVIPTVSTVAAQVKEMV
KQSGLPIEIVEGTKERHDAFKSGDVAIAASGTVALELAIVDVPHIIAYKVPKLTEWLARH
FIHIQFVNLSNILLGKEVVPELLQEDCSVEKIMYYVEQFMKHKPVYNQQMDGFEKVRKLL
GMGQQTPSDNAAEAIIRAVENYKK

Enzyme Prediction     

No EC number prediction in MGYG000000445_01380.

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GT19	4	360	2.1e-122	0.9887005649717514

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
PRK00025	lpxB	4.76e-148	1	381	2	377	lipid-A-disaccharide synthase; Reviewed
COG0763	LpxB	1.58e-140	1	376	2	376	Lipid A disaccharide synthetase [Cell wall/membrane/envelope biogenesis].
pfam02684	LpxB	6.53e-106	4	375	2	374	Lipid-A-disaccharide synthetase. This is a family of lipid-A-disaccharide synthetases, EC:2.4.2.128. These enzymes catalyze the reaction: UDP-2,3-bis(3-hydroxytetradecanoyl) glucosamine + 2,3-bis(3-hydroxytetradecanoyl)-beta-D-glucosaminyl 1-phosphate <=> UDP + 2,3-bis(3-hydroxytetradecanoyl)-D-glucosaminyl-1,6 -beta-D-2,3-bis(3-hydroxytetradecanoyl)-beta-D-glucosaminyl 1-phosphate. These enzymes catalyze the fist disaccharide step in the synthesis of lipid-A-disaccharide.
TIGR00215	lpxB	2.00e-78	5	332	10	337	lipid-A-disaccharide synthase. Lipid-A precursor biosynthesis producing lipid A disaccharide in a condensation reaction. transcribed as part of an operon including lpxA [Cell envelope, Biosynthesis and degradation of surface polysaccharides and lipopolysaccharides]
PRK14089	PRK14089	5.74e-52	1	333	2	310	lipid-A-disaccharide synthase.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
QIM10250.1	2.43e-174	1	380	5	385
ARJ65449.1	2.82e-113	4	384	9	391
BAE51288.1	3.88e-112	1	384	1	386
CUW40282.1	1.29e-111	4	384	9	391
AXO15096.1	1.08e-108	2	379	11	385

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
5W8N_A	3.35e-61	5	380	11	382	LipidA Disaccharide Synthase (LpxB)-6 solubilizing mutations [Escherichia coli BL21(DE3)]
5W8S_A	3.35e-61	5	380	11	382	LipidA Disaccharide Synthase (LpxB)-7 solubilizing mutations [Escherichia coli BL21(DE3)],5W8X_A Lipid A Disaccharide Synthase (LpxB)-7 solubilizing mutations-Bound to UDP [Escherichia coli BL21(DE3)]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
Q2W4D7	7.76e-113	1	384	1	386	Lipid-A-disaccharide synthase OS=Magnetospirillum magneticum (strain AMB-1 / ATCC 700264) OX=342108 GN=lpxB PE=3 SV=1
B6IST7	7.76e-105	4	379	7	383	Lipid-A-disaccharide synthase OS=Rhodospirillum centenum (strain ATCC 51521 / SW) OX=414684 GN=lpxB PE=3 SV=1
Q5FUA3	2.09e-88	4	376	32	402	Lipid-A-disaccharide synthase OS=Gluconobacter oxydans (strain 621H) OX=290633 GN=lpxB PE=3 SV=1
Q0BS63	4.32e-82	4	379	7	367	Lipid-A-disaccharide synthase OS=Granulibacter bethesdensis (strain ATCC BAA-1260 / CGDNIH1) OX=391165 GN=lpxB PE=3 SV=1
Q1QMM4	5.77e-77	2	379	12	389	Lipid-A-disaccharide synthase OS=Nitrobacter hamburgensis (strain DSM 10229 / NCIMB 13809 / X14) OX=323097 GN=lpxB PE=3 SV=1

SignalP and Lipop Annotations

This protein is predicted as OTHER

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
1.000066	0.000000	0.000000	0.000000	0.000000	0.000000

TMHMM  Annotations     

There is no transmembrane helices in MGYG000000445_01380.