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CAZyme Information: MGYG000000475_01296
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Basic Information |
Genomic context |
Full Sequence |
Enzyme annotations |
CAZy signature domains |
CDD domains |
CAZyme hits |
PDB hits |
Swiss-Prot hits |
SignalP and Lipop annotations |
TMHMM annotations
Basic Information help
| Species | Duodenibacillus sp900544335 | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Lineage | Bacteria; Proteobacteria; Gammaproteobacteria; Burkholderiales; Burkholderiaceae; Duodenibacillus; Duodenibacillus sp900544335 | |||||||||||
| CAZyme ID | MGYG000000475_01296 | |||||||||||
| CAZy Family | GH13 | |||||||||||
| CAZyme Description | Glycogen debranching enzyme | |||||||||||
| CAZyme Property |
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| Genome Property |
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| Gene Location | Start: 25253; End: 28720 Strand: + | |||||||||||
CAZyme Signature Domains help
| Family | Start | End | Evalue | family coverage |
|---|---|---|---|---|
| GH77 | 669 | 1149 | 5.5e-158 | 0.9898785425101214 |
| GH13 | 163 | 507 | 2.8e-152 | 0.9971509971509972 |
CDD Domains download full data without filtering help
| Cdd ID | Domain | E-Value | qStart | qEnd | sStart | sEnd | Domain Description |
|---|---|---|---|---|---|---|---|
| cd11326 | AmyAc_Glg_debranch | 0.0 | 126 | 548 | 1 | 433 | Alpha amylase catalytic domain found in glycogen debranching enzymes. Debranching enzymes facilitate the breakdown of glycogen through glucosyltransferase and glucosidase activity. These activities are performed by a single enzyme in mammals, yeast, and some bacteria, but by two distinct enzymes in Escherichia coli and other bacteria. Debranching enzymes perform two activities: 4-alpha-D-glucanotransferase (EC 2.4.1.25) and amylo-1,6-glucosidase (EC 3.2.1.33). 4-alpha-D-glucanotransferase catalyzes the endohydrolysis of 1,6-alpha-D-glucoside linkages at points of branching in chains of 1,4-linked alpha-D-glucose residues. Amylo-alpha-1,6-glucosidase catalyzes the endohydrolysis of 1,6-alpha-D-glucoside linkages at points of branching in chains of 1,4-linked alpha-D-glucose residues. In Escherichia coli, GlgX is the debranching enzyme and malQ is the 4-alpha-glucanotransferase. TreX, an archaeal glycogen-debranching enzyme has dual activities like mammals and yeast, but is structurally similar to GlgX. TreX exists in two oligomeric states, a dimer and tetramer. Isoamylase (EC 3.2.1.68) is one of the starch-debranching enzymes that catalyzes the hydrolysis of alpha-1,6-glucosidic linkages specific in alpha-glucans such as amylopectin or glycogen and their beta-limit dextrins. The Alpha-amylase family comprises the largest family of glycoside hydrolases (GH), with the majority of enzymes acting on starch, glycogen, and related oligo- and polysaccharides. These proteins catalyze the transformation of alpha-1,4 and alpha-1,6 glucosidic linkages with retention of the anomeric center. The protein is described as having 3 domains: A, B, C. A is a (beta/alpha) 8-barrel; B is a loop between the beta 3 strand and alpha 3 helix of A; C is the C-terminal extension characterized by a Greek key. The majority of the enzymes have an active site cleft found between domains A and B where a triad of catalytic residues (Asp, Glu and Asp) performs catalysis. Other members of this family have lost the catalytic activity as in the case of the human 4F2hc, or only have 2 residues that serve as the catalytic nucleophile and the acid/base, such as Thermus A4 beta-galactosidase with 2 Glu residues (GH42) and human alpha-galactosidase with 2 Asp residues (GH31). The family members are quite extensive and include: alpha amylase, maltosyltransferase, cyclodextrin glycotransferase, maltogenic amylase, neopullulanase, isoamylase, 1,4-alpha-D-glucan maltotetrahydrolase, 4-alpha-glucotransferase, oligo-1,6-glucosidase, amylosucrase, sucrose phosphorylase, and amylomaltase. |
| pfam02446 | Glyco_hydro_77 | 0.0 | 669 | 1132 | 1 | 458 | 4-alpha-glucanotransferase. These enzymes EC:2.4.1.25 transfer a segment of a (1,4)-alpha-D-glucan to a new 4-position in an acceptor, which may be glucose or (1,4)-alpha-D-glucan. |
| PRK14510 | PRK14510 | 0.0 | 18 | 1153 | 17 | 1220 | bifunctional glycogen debranching protein GlgX/4-alpha-glucanotransferase. |
| PRK14508 | PRK14508 | 0.0 | 661 | 1153 | 3 | 496 | 4-alpha-glucanotransferase; Provisional |
| PRK03705 | PRK03705 | 0.0 | 17 | 623 | 12 | 627 | glycogen debranching protein GlgX. |
CAZyme Hits help
| Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End |
|---|---|---|---|---|---|
| QKS28039.1 | 2.36e-214 | 5 | 657 | 6 | 672 |
| ABG03293.1 | 9.46e-187 | 18 | 595 | 17 | 625 |
| QJE01551.1 | 1.63e-186 | 6 | 654 | 4 | 694 |
| APW38239.1 | 1.66e-185 | 18 | 631 | 30 | 684 |
| BAU48061.1 | 6.56e-185 | 18 | 670 | 17 | 728 |
PDB Hits download full data without filtering help
| Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
|---|---|---|---|---|---|---|
| 2WSK_A | 4.42e-172 | 18 | 626 | 13 | 630 | Crystalstructure of Glycogen Debranching Enzyme GlgX from Escherichia coli K-12 [Escherichia coli K-12] |
| 2VNC_A | 2.77e-159 | 22 | 642 | 27 | 700 | Crystalstructure of Glycogen Debranching enzyme TreX from Sulfolobus solfataricus [Saccharolobus solfataricus],2VNC_B Crystal structure of Glycogen Debranching enzyme TreX from Sulfolobus solfataricus [Saccharolobus solfataricus],2VR5_A Crystal structure of Trex from Sulfolobus Solfataricus in complex with acarbose intermediate and glucose [Saccharolobus solfataricus],2VR5_B Crystal structure of Trex from Sulfolobus Solfataricus in complex with acarbose intermediate and glucose [Saccharolobus solfataricus],2VUY_A Crystal structure of Glycogen Debranching exzyme TreX from Sulfolobus solfatarius [Saccharolobus solfataricus],2VUY_B Crystal structure of Glycogen Debranching exzyme TreX from Sulfolobus solfatarius [Saccharolobus solfataricus],7EAV_A Chain A, Glycogen debranching enzyme [Saccharolobus solfataricus],7EAV_B Chain B, Glycogen debranching enzyme [Saccharolobus solfataricus] |
| 2X1I_A | 1.00e-137 | 661 | 1153 | 3 | 499 | glycosidehydrolase family 77 4-alpha-glucanotransferase from thermus brockianus [Thermus brockianus] |
| 1CWY_A | 4.60e-134 | 661 | 1153 | 3 | 499 | CrystalStructure Of Amylomaltase From Thermus Aquaticus, A Glycosyltransferase Catalysing The Production Of Large Cyclic Glucans [Thermus aquaticus],1ESW_A X-Ray Structure Of Acarbose Bound To Amylomaltase From Thermus Aquaticus. Implications For The Synthesis Of Large Cyclic Glucans [Thermus aquaticus] |
| 1FP8_A | 6.44e-134 | 661 | 1153 | 3 | 499 | StructureOf The Amylomaltase From Thermus Thermophilus Hb8 In Space Group P21212 [Thermus thermophilus],1FP9_A Structure Of Amylomaltase From Thermus Thermophilus Hb8 In Space Group C2 [Thermus thermophilus] |
Swiss-Prot Hits download full data without filtering help
| Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
|---|---|---|---|---|---|---|
| A6TF50 | 1.23e-179 | 7 | 626 | 1 | 630 | Glycogen debranching enzyme OS=Klebsiella pneumoniae subsp. pneumoniae (strain ATCC 700721 / MGH 78578) OX=272620 GN=glgX PE=3 SV=1 |
| B5XTQ8 | 8.16e-177 | 7 | 626 | 1 | 630 | Glycogen debranching enzyme OS=Klebsiella pneumoniae (strain 342) OX=507522 GN=glgX PE=3 SV=1 |
| A9MTV3 | 1.62e-176 | 18 | 626 | 13 | 630 | Glycogen debranching enzyme OS=Salmonella paratyphi B (strain ATCC BAA-1250 / SPB7) OX=1016998 GN=glgX PE=3 SV=1 |
| B5FKF6 | 2.28e-176 | 18 | 626 | 13 | 630 | Glycogen debranching enzyme OS=Salmonella dublin (strain CT_02021853) OX=439851 GN=glgX PE=3 SV=1 |
| Q8ZLG6 | 2.28e-176 | 18 | 626 | 13 | 630 | Glycogen debranching enzyme OS=Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720) OX=99287 GN=glgX PE=3 SV=1 |
SignalP and Lipop Annotations help
This protein is predicted as OTHER
| Other | SP_Sec_SPI | LIPO_Sec_SPII | TAT_Tat_SPI | TATLIP_Sec_SPII | PILIN_Sec_SPIII |
|---|---|---|---|---|---|
| 0.998900 | 0.000997 | 0.000103 | 0.000002 | 0.000001 | 0.000003 |