CAZyme Information: MGYG000000487_01175

Basic Information

• Species: CAG-115 sp900768125
• Lineage: Bacteria; Firmicutes_A; Clostridia; Oscillospirales; Ruminococcaceae; CAG-115; CAG-115 sp900768125
• CAZyme ID: MGYG000000487_01175
• CAZy Family: GT51
• CAZyme Description: Penicillin-binding protein 4

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
765	MGYG000000487_9|CGC1	85814.71	4.956

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000000487	2815466	MAG	Fiji	Oceania

• Gene Location: Start: 77747; End: 80044 Strand: +

Full Sequence      

MNKKKIKYIRIREEQPKKRSSKKSRSRAAKNFGHAMTIIGTTFSAMLLILVIMVCIVATV
LAVYVLDFADNSYDANLRDIEMKYTSFIYGYDEDDQLVEIKRLATEQNRVWVDYEDIPQC
LVDAAVASEDKRFYTHKGVDWYRTAGAFFSNGDSGGGSTITQQLVKNLTGDKEVSWERKL
REIFRALSLEEKYTKIDILESYLNQIWFDGMVYGVGAASEYYFGKSVSDLNIGEAAVLAG
MIRNPYKMSPYYDLARSKQQQNYSLRCMYEQGMITTKEYEDYRYNRIQVHFAKPVYGDAY
GYIDERTLNDSTDDSDLDPSVQDKPYEAYKWNGDYTVSQDWYVDAAIDQVINDFADLKGI
TYTSAKNELYNGGYKIYINENMKLQKILEEKYRDPYTVLSSYNPAAAEKDLIQSAFIIID
YTGTVQAVVGGLGEKPGDGAFNRATQATRSPGSTMKPISTYSLAVQKNLITYSTLIPDEA
INIGTADEPQIWPQNYGEIWGDGTLRPAWYGVQHSLNTLAVRVGRMNTTRAMYTQLTQML
GFTTLVESDIALSPLCLGALTNGAKLVEVAGAYQIFGNGGIYYRPMFYSKVEDSKGNVIM
EQDFYGTQAVDSDTAWVTNRMIYKVVNGDSGTGPKAKLPNVEVVGKTGTSNAGTDLLFMG
LTPEYIGGLWIGYDDSRVIGGADGWKAVAQIWHDVMIDIQDTSSVLKFSPDPSVVERQYC
TMTGLLATNKCDSTSTGYYRTSNLPDYCNGDHGTIRKQIKANNPQ

Enzyme Prediction     

No EC number prediction in MGYG000000487_01175.

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GT51	100	269	8e-61	0.9717514124293786

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
TIGR02074	PBP_1a_fam	4.97e-148	108	696	1	525	penicillin-binding protein, 1A family. Bacterial that synthesize a cell wall of peptidoglycan (murein) generally have several transglycosylases and transpeptidases for the task. This family consists of bifunctional transglycosylase/transpeptidase penicillin-binding proteins (PBP). In the Proteobacteria, this family includes PBP 1A but not the paralogous PBP 1B (TIGR02071). This family also includes related proteins, often designated PBP 1A, from other bacterial lineages. [Cell envelope, Biosynthesis and degradation of murein sacculus and peptidoglycan]
COG0744	MrcB	8.86e-144	47	696	14	593	Membrane carboxypeptidase (penicillin-binding protein) [Cell wall/membrane/envelope biogenesis].
COG5009	MrcA	6.95e-116	46	739	8	758	Membrane carboxypeptidase/penicillin-binding protein [Cell wall/membrane/envelope biogenesis].
pfam00912	Transgly	1.76e-72	106	269	10	177	Transglycosylase. The penicillin-binding proteins are bifunctional proteins consisting of transglycosylase and transpeptidase in the N- and C-terminus respectively. The transglycosylase domain catalyzes the polymerization of murein glycan chains.
COG4953	PbpC	6.45e-62	56	733	8	594	Membrane carboxypeptidase/penicillin-binding protein PbpC [Cell wall/membrane/envelope biogenesis].

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
CBL33874.1	1.86e-238	18	752	23	758
CBK97274.1	7.79e-235	18	752	23	758
CCO04758.1	1.61e-149	17	752	10	740
ADU22273.1	2.47e-133	23	748	10	737
BCI59848.1	1.10e-128	36	755	38	743

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
3ZG8_B	8.76e-51	189	676	3	435	CrystalStructure of Penicillin Binding Protein 4 from Listeria monocytogenes in the Ampicillin bound form [Listeria monocytogenes],3ZG9_B Crystal Structure of Penicillin-Binding Protein 4 from Listeria monocytogenes in the Cefuroxime bound form [Listeria monocytogenes],3ZGA_B Crystal Structure of Penicillin-Binding Protein 4 from Listeria monocytogenes in the Carbenicillin bound form [Listeria monocytogenes]
7U4H_A	5.23e-50	92	739	25	801	ChainA, Penicillin-binding protein 1A (Pbp1a) [Chlamydia trachomatis D/UW-3/CX],7U4H_B Chain B, Penicillin-binding protein 1A (Pbp1a) [Chlamydia trachomatis D/UW-3/CX]
3ZG7_B	3.49e-46	189	676	3	435	CrystalStructure of Penicillin-Binding Protein 4 from Listeria monocytogenes in the apo form [Listeria monocytogenes]
2JE5_A	1.97e-41	100	724	45	662	StructuralAnd Mechanistic Basis Of Penicillin Binding Protein Inhibition By Lactivicins [Streptococcus pneumoniae R6],2JE5_B Structural And Mechanistic Basis Of Penicillin Binding Protein Inhibition By Lactivicins [Streptococcus pneumoniae R6]
5FGZ_A	4.27e-41	94	690	141	687	E.coli PBP1b in complex with FPI-1465 [Escherichia coli K-12],5HL9_A E. coli PBP1b in complex with acyl-ampicillin and moenomycin [Escherichia coli K-12],5HLA_A E. coli PBP1b in complex with acyl-cephalexin and moenomycin [Escherichia coli K-12],5HLB_A E. coli PBP1b in complex with acyl-aztreonam and moenomycin [Escherichia coli K-12],5HLD_A E. coli PBP1b in complex with acyl-CENTA and moenomycin [Escherichia coli K-12],6YN0_A Structure of E. coli PBP1b with a FtsN peptide activating transglycosylase activity [Escherichia coli K-12],7LQ6_A Chain A, Penicillin-binding protein 1B [Escherichia coli K-12]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
A7GHV1	3.16e-72	91	728	60	672	Penicillin-binding protein 1A OS=Clostridium botulinum (strain Langeland / NCTC 10281 / Type F) OX=441772 GN=pbpA PE=3 SV=1
Q891X1	4.61e-72	91	745	64	706	Penicillin-binding protein 1A OS=Clostridium tetani (strain Massachusetts / E88) OX=212717 GN=pbpA PE=3 SV=1
A7FY32	8.64e-72	91	728	60	672	Penicillin-binding protein 1A OS=Clostridium botulinum (strain ATCC 19397 / Type A) OX=441770 GN=pbpA PE=3 SV=1
A5I6G4	8.64e-72	91	728	60	672	Penicillin-binding protein 1A OS=Clostridium botulinum (strain Hall / ATCC 3502 / NCTC 13319 / Type A) OX=441771 GN=pbpA PE=3 SV=1
Q8XJ01	1.44e-68	59	684	44	651	Penicillin-binding protein 1A OS=Clostridium perfringens (strain 13 / Type A) OX=195102 GN=pbpA PE=3 SV=1

SignalP and Lipop Annotations

This protein is predicted as OTHER

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
0.999199	0.000801	0.000002	0.000002	0.000001	0.000002

TMHMM  Annotations     

start	end
43	65