CAZyme Information: MGYG000000493_00406

Basic Information

• Species: UBA2883 sp002103075
• Lineage: Bacteria; Cyanobacteria; Vampirovibrionia; Gastranaerophilales; Gastranaerophilaceae; UBA2883; UBA2883 sp002103075
• CAZyme ID: MGYG000000493_00406
• CAZy Family: GT9
• CAZyme Description: Lipopolysaccharide core heptosyltransferase RfaQ

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
328	MGYG000000493_2|CGC2	36949.37	8.8039

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000000493	1815977	MAG	Fiji	Oceania

• Gene Location: Start: 122608; End: 123594 Strand: -

Full Sequence      

MIKRILAINFGGLGDEILFLPALISLKKEFPYAKITLALEPRSKAIQQLSDMVDDVILVD
LKRKNKYIELFGLVTKAIFGDFQMVIASGANPMMSVIETMTFIPKRYGYDTGKLSRWLLT
KAIPLNKNQYAGNMYHDLVSDVTDCKTELPEINSPKVDKIPNTVLIHPGVSLMSKEKGCI
KSITSREWAKLIDMLAQRGKRVLLAGGPDDKQCIEEIVQYSQTKQFENLFGKTKNLQDLA
KLISASERFVCSDSAPLHIAVALKTRTFAFFGPTDDRKLIPQNEFVTALKTNDGCPLKPC
LWERRMTSCEELKCLKYDFDKLVETILG

Enzyme Prediction     

No EC number prediction in MGYG000000493_00406.

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GT9	81	303	5.3e-30	0.8977777777777778

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
COG0859	RfaF	3.39e-44	2	295	1	301	ADP-heptose:LPS heptosyltransferase [Cell wall/membrane/envelope biogenesis].
cd03789	GT9_LPS_heptosyltransferase	1.17e-41	4	316	1	264	lipopolysaccharide heptosyltransferase and similar proteins. Lipopolysaccharide heptosyltransferase (2.4.99.B6) is involved in the biosynthesis of lipooligosaccharide (LOS). Lipopolysaccharide (LPS) is a major component of the outer membrane of gram-negative bacteria. LPS heptosyltransferase transfers heptose molecules from ADP-heptose to 3-deoxy-D-manno-octulosonic acid (KDO), a part of the inner core component of LPS. This family also contains lipopolysaccharide 1,2-N-acetylglucosaminetransferase EC 2.4.1.56 and belongs to the GT-B structural superfamily of glycoslytransferases, which have characteristic N- and C-terminal domains each containing a typical Rossmann fold. The two domains have high structural homology despite minimal sequence homology. The large cleft that separates the two domains includes the catalytic center and permits a high degree of flexibility.
pfam01075	Glyco_transf_9	5.52e-18	95	313	26	246	Glycosyltransferase family 9 (heptosyltransferase). Members of this family belong to glycosyltransferase family 9. Lipopolysaccharide is a major component of the outer leaflet of the outer membrane in Gram-negative bacteria. It is composed of three domains; lipid A, Core oligosaccharide and the O-antigen. All of these enzymes transfer heptose to the lipopolysaccharide core.
PRK10422	PRK10422	7.15e-18	3	275	6	292	lipopolysaccharide core biosynthesis protein; Provisional
TIGR02195	heptsyl_trn_II	6.07e-14	103	316	101	318	lipopolysaccharide heptosyltransferase II. This family consists of examples of ADP-heptose:LPS heptosyltransferase II, an enzyme of LPS inner core region biosynthesis. LPS, composed of lipid A, a core region, and O antigen, is found in the outer membrane of Gram-negative bacteria. [Cell envelope, Biosynthesis and degradation of surface polysaccharides and lipopolysaccharides]

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
AOR38411.1	3.52e-138	4	328	2	328
AFY67702.1	6.45e-50	4	291	2	299
BAI93916.1	4.32e-47	4	294	2	303
QNH55727.1	4.32e-47	4	294	2	303
AMW31030.1	4.32e-47	4	294	2	303

PDB Hits     

has no PDB hit.

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
Q9R9D5	2.29e-14	14	275	5	280	Lipopolysaccharide core heptosyltransferase RfaQ OS=Escherichia coli OX=562 GN=rfaQ PE=1 SV=1
P25742	3.57e-13	14	275	5	280	Lipopolysaccharide core heptosyltransferase RfaQ OS=Escherichia coli (strain K12) OX=83333 GN=rfaQ PE=3 SV=2

SignalP and Lipop Annotations

This protein is predicted as OTHER

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
1.000054	0.000000	0.000000	0.000000	0.000000	0.000000

TMHMM  Annotations     

There is no transmembrane helices in MGYG000000493_00406.