CAZyme Information: MGYG000000501_00516

Basic Information

• Species: CAG-127 sp900553925
• Lineage: Bacteria; Firmicutes_A; Clostridia; Lachnospirales; Lachnospiraceae; CAG-127; CAG-127 sp900553925
• CAZyme ID: MGYG000000501_00516
• CAZy Family: GT2
• CAZyme Description: hypothetical protein

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
639	MGYG000000501_2|CGC2	73296.96	5.2239

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000000501	2730672	MAG	Fiji	Oceania

• Gene Location: Start: 196456; End: 198375 Strand: +

Full Sequence      

MKFRLTDLNTETIKKGIAHMKETTIPEALSKARSMVTGLDLAYDRFFREELEADEEELQS
QRERMFAYAPFISVIVPVYMTPERSLRAMIESVLHQTYENWELCMVDGSQAKGTPPVLDA
HRTEDGTPTVMEKVYSLETERIIRQYQEDHPQIHYVLMEENRGISGNINYALQMAHGEYV
AFLDHDDVLTEDAIYRIVVKLQEQRYDIVYSDEDRMSENGNQYKEPRFKPDFDMELLRTC
NYIGHFCMIRRSLLLEKGGFRSEYDGAQDYDMLLRCVGHGTKVGHIGRVLYHKRIHDGTS
PERDSKHAQENQAGFAALEDYVGREHLLAGVTMSVRRNVYNIKYDTPGNPLVSIIVTGSG
TREQMTQMLAPFYEKTRYSNFEIILVDEAHSDDGLQKYYQQLQNQRRNVIVVPAQPGKTK
AEYRNLGSLRAGGNYLLFLDAGIGIDAPTALGEMLGICMQEDVAVVSGCVYDEKEQLLYA
GVRLAGKFETPVQGWQRKTEREIDLSDVQNLYQIPYRGMRRIYETDGRAEIRTDETGARR
YYGLNREYQAVLGSCMLVKKKVFAQAGGFSDKFRSELSSLDFCLRIGERGYRIVNAAHAP
WKLSIPSMDVDGEDTLPADEARTEQELFEILWSHILPFC

Enzyme Prediction     

No EC number prediction in MGYG000000501_00516.

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GT2	138	254	1.7e-20	0.7352941176470589

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
cd04184	GT2_RfbC_Mx_like	4.33e-88	70	295	1	202	Myxococcus xanthus RfbC like proteins are required for O-antigen biosynthesis. The rfbC gene encodes a predicted protein of 1,276 amino acids, which is required for O-antigen biosynthesis in Myxococcus xanthus. It is a subfamily of Glycosyltransferase Family GT2, which includes diverse families of glycosyl transferases with a common GT-A type structural fold, which has two tightly associated beta/alpha/beta domains that tend to form a continuous central sheet of at least eight beta-strands. These are enzymes that catalyze the transfer of sugar moieties from activated donor molecules to specific acceptor molecules, forming glycosidic bonds.
cd06433	GT_2_WfgS_like	8.47e-30	73	300	1	200	WfgS and WfeV are involved in O-antigen biosynthesis. Escherichia coli WfgS and Shigella dysenteriae WfeV are glycosyltransferase 2 family enzymes involved in O-antigen biosynthesis. GT-2 enzymes have GT-A type structural fold, which has two tightly associated beta/alpha/beta domains that tend to form a continuous central sheet of at least eight beta-strands. These are enzymes that catalyze the transfer of sugar moieties from activated donor molecules to specific acceptor molecules, forming glycosidic bonds. Glycosyltransferases have been classified into more than 90 distinct sequence based families.
pfam00535	Glycos_transf_2	1.07e-28	73	254	1	164	Glycosyl transferase family 2. Diverse family, transferring sugar from UDP-glucose, UDP-N-acetyl- galactosamine, GDP-mannose or CDP-abequose, to a range of substrates including cellulose, dolichol phosphate and teichoic acids.
cd00761	Glyco_tranf_GTA_type	8.70e-26	74	286	1	156	Glycosyltransferase family A (GT-A) includes diverse families of glycosyl transferases with a common GT-A type structural fold. Glycosyltransferases (GTs) are enzymes that synthesize oligosaccharides, polysaccharides, and glycoconjugates by transferring the sugar moiety from an activated nucleotide-sugar donor to an acceptor molecule, which may be a growing oligosaccharide, a lipid, or a protein. Based on the stereochemistry of the donor and acceptor molecules, GTs are classified as either retaining or inverting enzymes. To date, all GT structures adopt one of two possible folds, termed GT-A fold and GT-B fold. This hierarchy includes diverse families of glycosyl transferases with a common GT-A type structural fold, which has two tightly associated beta/alpha/beta domains that tend to form a continuous central sheet of at least eight beta-strands. The majority of the proteins in this superfamily are Glycosyltransferase family 2 (GT-2) proteins. But it also includes families GT-43, GT-6, GT-8, GT13 and GT-7; which are evolutionarily related to GT-2 and share structure similarities.
cd06423	CESA_like	2.84e-18	74	261	1	173	CESA_like is the cellulose synthase superfamily. The cellulose synthase (CESA) superfamily includes a wide variety of glycosyltransferase family 2 enzymes that share the common characteristic of catalyzing the elongation of polysaccharide chains. The members include cellulose synthase catalytic subunit, chitin synthase, glucan biosynthesis protein and other families of CESA-like proteins. Cellulose synthase catalyzes the polymerization reaction of cellulose, an aggregate of unbranched polymers of beta-1,4-linked glucose residues in plants, most algae, some bacteria and fungi, and even some animals. In bacteria, algae and lower eukaryotes, there is a second unrelated type of cellulose synthase (Type II), which produces acylated cellulose, a derivative of cellulose. Chitin synthase catalyzes the incorporation of GlcNAc from substrate UDP-GlcNAc into chitin, which is a linear homopolymer of beta-(1,4)-linked GlcNAc residues and Glucan Biosynthesis protein catalyzes the elongation of beta-1,2 polyglucose chains of Glucan.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
QNL99672.1	3.44e-298	1	636	1	639
QWT52369.1	1.71e-286	1	636	1	639
QNM10257.1	2.06e-101	8	598	13	543
QTU82747.1	1.75e-98	1	637	324	900
CBL21583.1	2.10e-97	8	594	19	546

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
3BCV_A	4.44e-07	70	220	5	128	Crystalstructure of a putative glycosyltransferase from Bacteroides fragilis [Bacteroides fragilis NCTC 9343],3BCV_B Crystal structure of a putative glycosyltransferase from Bacteroides fragilis [Bacteroides fragilis NCTC 9343]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
P55465	1.72e-57	60	627	356	867	Uncharacterized protein y4gI OS=Sinorhizobium fredii (strain NBRC 101917 / NGR234) OX=394 GN=NGR_a03550 PE=4 SV=1
Q50864	6.02e-41	70	597	319	786	O-antigen biosynthesis protein RfbC OS=Myxococcus xanthus OX=34 GN=rfbC PE=4 SV=1
Q58457	1.77e-15	70	305	8	218	Uncharacterized glycosyltransferase MJ1057 OS=Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440) OX=243232 GN=MJ1057 PE=3 SV=2
O32268	2.13e-10	65	220	1	130	Putative teichuronic acid biosynthesis glycosyltransferase TuaG OS=Bacillus subtilis (strain 168) OX=224308 GN=tuaG PE=2 SV=1
P77414	3.23e-08	70	305	5	222	Putative colanic acid biosynthesis glycosyl transferase WcaA OS=Escherichia coli (strain K12) OX=83333 GN=wcaA PE=4 SV=1

SignalP and Lipop Annotations

This protein is predicted as OTHER

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
1.000065	0.000000	0.000000	0.000000	0.000000	0.000000

TMHMM  Annotations     

There is no transmembrane helices in MGYG000000501_00516.