CAZyme Information: MGYG000000501_01850

Basic Information

• Species: CAG-127 sp900553925
• Lineage: Bacteria; Firmicutes_A; Clostridia; Lachnospirales; Lachnospiraceae; CAG-127; CAG-127 sp900553925
• CAZyme ID: MGYG000000501_01850
• CAZy Family: CBM48
• CAZyme Description: 1,4-alpha-glucan branching enzyme GlgB

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
787	MGYG000000501_11|CGC3	90447.98	6.5471

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000000501	2730672	MAG	Fiji	Oceania

• Gene Location: Start: 106940; End: 109303 Strand: +

Full Sequence      

MNKRLEDWVNWILIDELKNGIINAPKHLLGNHDYGDGQVIMVYRPHAEKVCVVSPTGKNR
DEMECLLDGVYGFYSAKKKYKGTKYRIETTYQDGTTVVTADAYAFDSQITDFDTYLFAEG
KHYDVYEKFGAHPKTIDGVKGTYFAVWAPHARRVSVVGDFNMWDGALNPMQMMQTSGIYE
LFIPDVAPGAVYKYQILTRDGEILYKADPYGNQCQVRPDNANVVADLTGYKWKDTGWIDN
RKKQTRETELKKPMAIYECHLGSWKKKIEDSDFGFYTYREMAKMLCDYVKKMGYTHVELM
GIAEYPFDGSWGYQVTNYYAPTSRYGSPEDFMYFVDHMHENGIGVILDWVPAHFPRDAHG
LGRFDGMPLYEHPDPRRGEHPDWGTYIFDFGRNEVSNFLTANALFWVEKFHVDALRVDAV
ASMLYLDYGKQDGQWLPNKDGGNENYDAIELLRKINTVMEERNPGAFLIAEESTAWAGVT
APASMKGLGFLYKWNMGWMNDFLEYMKMDPYFRSFNHNRLTFSLSYTYAENYVLVISHDE
VVHLKCSMLNKMPGTEMDKFANLRTAYGFMYGHPGKKLLFMGQEFGQLREWSEARSLDWF
LLDQPLHKKMQKWVADLNHMYTTYDACYYNDNNQMGFEWTKVDDANTSIVAFVRRGKTVK
DQLLFVCNFVPVERKDYWIGVPCLTEYEEIINSDAKIYGGSGTKNGTVKAFEEKCDRMPY
AISIDIAPLSMMVFKYDYVDKKPAVADAPATAAIEKKPEPKKAATAKKVAAVKTSAGKKP
VEKKTTK

Enzyme Prediction     

EC	2.4.1.18

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GH13	287	587	7.2e-154	0.9966777408637874
CBM48	128	213	1.7e-18	0.8947368421052632

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
PRK12313	PRK12313	0.0	107	736	5	627	1,4-alpha-glucan branching protein GlgB.
PRK14705	PRK14705	0.0	14	735	508	1221	glycogen branching enzyme; Provisional
COG0296	GlgB	0.0	104	736	1	628	1,4-alpha-glucan branching enzyme [Carbohydrate transport and metabolism].
PRK14706	PRK14706	0.0	129	736	27	621	glycogen branching enzyme; Provisional
PRK05402	PRK05402	0.0	14	737	6	724	1,4-alpha-glucan branching protein GlgB.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
QNL99342.1	0.0	1	787	1	767
QWT52740.1	0.0	1	761	1	761
CBK82088.1	0.0	1	769	1	762
BBF44560.1	0.0	1	742	1	742
ANV77640.1	5.54e-291	14	735	7	730

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
5GR5_A	1.27e-243	14	737	30	774	Crystalstructure of branching enzyme W610A mutant from Cyanothece sp. ATCC 51142 [Crocosphaera subtropica ATCC 51142]
5GR1_A	2.54e-243	14	737	30	774	Crystalstructure of branching enzyme Y500A/D501A mutant from Cyanothece sp. ATCC 51142 in complex with maltoheptaose [Crocosphaera subtropica ATCC 51142],5GR6_A Crystal structure of branching enzyme Y500A/D501A double mutant from Cyanothece sp. ATCC 51142 [Crocosphaera subtropica ATCC 51142]
5GR2_A	5.10e-243	14	737	30	774	Crystalstructure of branching enzyme L541A mutant from Cyanothece sp. ATCC 51142 [Crocosphaera subtropica ATCC 51142],5GR4_A Crystal structure of branching enzyme L541A mutant from Cyanothece sp. ATCC 51142 in complex with maltoheptaose [Crocosphaera subtropica ATCC 51142]
5GQZ_A	5.10e-243	14	737	30	774	Crystalstructure of branching enzyme Y500A mutant from Cyanothece sp. ATCC 51142 [Crocosphaera subtropica ATCC 51142]
5GR0_A	7.21e-243	14	737	30	774	Crystalstructure of branching enzyme D501A mutant from Cyanothece sp. ATCC 51142 [Crocosphaera subtropica ATCC 51142]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
P30539	6.38e-266	109	736	7	632	1,4-alpha-glucan branching enzyme GlgB OS=Butyrivibrio fibrisolvens OX=831 GN=glgB PE=1 SV=1
Q7NL20	1.29e-256	14	735	10	727	1,4-alpha-glucan branching enzyme GlgB OS=Gloeobacter violaceus (strain ATCC 29082 / PCC 7421) OX=251221 GN=glgB PE=3 SV=1
Q1AZ86	2.03e-255	25	735	18	718	1,4-alpha-glucan branching enzyme GlgB OS=Rubrobacter xylanophilus (strain DSM 9941 / NBRC 16129 / PRD-1) OX=266117 GN=glgB PE=3 SV=1
P52981	1.49e-254	9	735	5	751	1,4-alpha-glucan branching enzyme GlgB OS=Synechocystis sp. (strain PCC 6803 / Kazusa) OX=1111708 GN=glgB PE=3 SV=1
Q8DLB8	6.00e-253	14	737	8	751	1,4-alpha-glucan branching enzyme GlgB OS=Thermosynechococcus vestitus (strain IAM M-273 / NIES-2133 / BP-1) OX=197221 GN=glgB PE=3 SV=1

SignalP and Lipop Annotations

This protein is predicted as OTHER

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
1.000056	0.000000	0.000000	0.000000	0.000000	0.000000

TMHMM  Annotations     

There is no transmembrane helices in MGYG000000501_01850.