CAZyme Information: MGYG000000504_00302

Basic Information

• Species: CAG-312 sp000438015
• Lineage: Bacteria; Verrucomicrobiota; Verrucomicrobiae; Opitutales; CAG-312; CAG-312; CAG-312 sp000438015
• CAZyme ID: MGYG000000504_00302
• CAZy Family: GH33
• CAZyme Description: hypothetical protein

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
595		67329.04	7.3379

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000000504	2519783	MAG	Fiji	Oceania

• Gene Location: Start: 52820; End: 54607 Strand: +

Full Sequence      

MTRAAKILSILFILFCAHLSSALNLSDDWRYAPNGNSIYDNGYVDQPYMLVLPDGRWFCA
FTTAEGEEGSKGQHIVSMVSSDNGKTWSKPVQIEKPDAPSSSWATPFLTDYGRIYVFYDF
NGSKVDSLNGKKIVRDDMLGWYCFRYSDDFGSTWSERHRLPVRITRCDRENDWKGEVQIF
WGIDKPKRLSNGAVAFCFSKLGKYLQEKGEGWMFRCDNIQTERDPKKLDWKMLPEDDEGI
RNPKFGSIQEEHNLVELSDGSLYCVYRTTLGHPACSYSRDGGKTWSEPDFVRYPDGRPLK
TPRACPKLYKAKNGKFLLWFHNHSQRGLINRNPVWISGGVEKNGKIEWSQPEILLHNPED
TTPETGRYSYPDFIENNGRYWVSETQKVKARVREIPAGFLEALWNQADSKAAASGGIFFE
DKDIPKGGKTINAVRLPKLDKSSFTMEFVVSFDKLEKGDAIYRGMNPFGGGTELLVGGNS
SIALTLSNYRINFTYLSDISSIAENKTHHIVLIVDAYARIVSCVIDGKLCDGGEYAYCGW
ARFDKTMTDVNCWAQNSEIGVSENLKVEAVRFYNRALTVSEAIGNFNALKSKGSL

Enzyme Prediction     

No EC number prediction in MGYG000000504_00302.

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GH33	47	376	9.5e-24	0.8538011695906432

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
cd15482	Sialidase_non-viral	1.87e-25	49	382	27	320	Non-viral sialidases. Sialidases or neuraminidases function to bind and hydrolyze terminal sialic acid residues from various glycoconjugates, they play vital roles in pathogenesis, bacterial nutrition and cellular interactions. They have a six-bladed, beta-propeller fold with the non-viral sialidases containing 2-5 Asp-box motifs (most commonly Ser/Thr-X-Asp-[X]-Gly-X-Thr- Trp/Phe). This CD includes eubacterial and eukaryotic sialidases.
pfam13088	BNR_2	9.33e-15	68	380	13	277	BNR repeat-like domain. This family of proteins contains BNR-like repeats suggesting these proteins may act as sialidases.
pfam13088	BNR_2	1.29e-06	46	160	164	257	BNR repeat-like domain. This family of proteins contains BNR-like repeats suggesting these proteins may act as sialidases.
cd08979	GH_J	0.005	33	130	118	211	Glycosyl hydrolase families 32 and 68, which form the clan GH-J. This glycosyl hydrolase family clan J (according to carbohydrate-active enzymes database (CAZY)) includes family 32 (GH32) and 68 (GH68). GH32 enzymes include invertase (EC 3.2.1.26) and other other fructofuranosidases such as inulinase (EC 3.2.1.7), exo-inulinase (EC 3.2.1.80), levanase (EC 3.2.1.65), and transfructosidases such sucrose:sucrose 1-fructosyltransferase (EC 2.4.1.99), fructan:fructan 1-fructosyltransferase (EC 2.4.1.100), sucrose:fructan 6-fructosyltransferase (EC 2.4.1.10), fructan:fructan 6G-fructosyltransferase (EC 2.4.1.243) and levan fructosyltransferases (EC 2.4.1.-). The GH68 family consists of frucosyltransferases (FTFs) that include levansucrase (EC 2.4.1.10, also known as beta-D-fructofuranosyl transferase), beta-fructofuranosidase (EC 3.2.1.26) and inulosucrase (EC 2.4.1.9). GH32 and GH68 family enzymes are retaining enzymes (i.e. they retain the configuration at anomeric carbon atom of the substrate) and catalyze hydrolysis in two steps involving a covalent glycosyl enzyme intermediate: an aspartate located close to the N-terminus acts as the catalytic nucleophile and a glutamate acts as the general acid/base; a conserved aspartate residue in the Arg-Asp-Pro (RDP) motif stabilizes the transition state. A common structural feature of all these enzymes is a 5-bladed beta-propeller domain, similar to GH43, that contains the catalytic acid and catalytic base. A long V-shaped groove, partially enclosed at one end, forms a single extended substrate-binding surface across the face of the propeller.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
QHV94280.1	1.59e-200	35	592	36	588
QIP17358.1	6.80e-197	35	592	36	588
QGY47823.1	2.66e-148	2	588	3	595
BCS36327.1	6.03e-143	26	588	20	572
QDU54552.1	1.56e-114	19	404	41	435

PDB Hits     

has no PDB hit.

Swiss-Prot Hits     

has no Swissprot hit.

SignalP and Lipop Annotations

This protein is predicted as SP

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
0.000239	0.999111	0.000175	0.000160	0.000147	0.000144

TMHMM  Annotations     

There is no transmembrane helices in MGYG000000504_00302.