CAZyme Information: MGYG000000516_00765

Basic Information

• Lineage: Bacteria; Firmicutes_A; Clostridia; Oscillospirales; CAG-272; UMGS902
• CAZyme ID: MGYG000000516_00765
• CAZy Family: GH43
• CAZyme Description: hypothetical protein

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
797		85223.33	4.8371

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000000516	1853543	MAG	Fiji	Oceania

• Gene Location: Start: 3092; End: 5485 Strand: +

Full Sequence      

MKKTLLMLACALLLALTMTAAVGAYEFAPGSDAVVYVDGTAGSDANSGATPESAVATPQK
AQSLLPQGGTVVVSGATALTTAYSPDAVNGAVLYTSVYGGTDYRTDGTKLSVGGNMAFSN
DTYFDNIDLAITKSALVFAGKFHNFGFGAGVRCEQAATAATFHYPVILAGVNTPASLAAA
NADADFTLTIAGGTWRSFYGGNRRTSAAQVLGKVSGDSYIRIEGGTFTGSGGEYASLGGA
NIHTGRMVLEITGGVFDCEFFMFRRTQARPTDTSKDAGGNGTAQAVFLIRGGQFNNHIAS
GPNATIAGSLRNYANLTMVVTGGSFADDMKLFNRSVMGTVLIRCSAETEAMLRPHIENIH
NFLFSNDLSAAETETGTAHFAGMISDTPDPFVFEKDGMYYIAFSDGNGISVSASREMVFS
RFTYNLVKVFNNTMTDIANAKHEYWAPEIHYFSADEVGTADAGWYIYFAADDGYDENHRM
YVLRATDPEDAQSTYKMVGELKTAEDCWAIDGTVMKLGGKLYFVWSGHAANNSTQQIYIQ
EMESPSKMKDGARTLLAAPTESWERRNSPLLEGPQALVLDGTAHIVYSANGSWTRRYCLG
MLTLVGDNPLDASAWQKSDGPVFEMSEENAMFGTGHASFVQSSIDGTYWVYYHAVPDASV
KDGNDASGTPVFWQARRTFAQQFTTETKRVGGKTLTYPVFGAPLAASTARDVSIHTPDKC
GDTHFYGADGETCLICRTKKNAPPAPAPDLRYGDMDSDGKIDLVDALLLVRTMINGGNDA
TLTDVLALLRYIVVGSN

Enzyme Prediction     

No EC number prediction in MGYG000000516_00765.

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GH43	384	687	3.7e-78	0.9659863945578231

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
cd18820	GH43_LbAraf43-like	4.95e-104	388	655	1	258	Glycosyl hydrolase family 43 proteins similar to Lactobacillus brevis alpha-L-arabinofuranosidase LbAraf43 and Geobacillus thermoleovorans GbtXyl43B. This uncharacterized glycosyl hydrolase family 43 (GH43) subgroup belongs to a subgroup which includes enzymes with beta-xylosidase (EC 3.2.1.37), alpha-L-arabinofuranosidase (EC 3.2.1.55) and possibly bifunctional xylosidase/arabinofuranosidase activities, similar to Lactobacillus brevis alpha-L-arabinofuranosidase LbAraf43 and Geobacillus thermoleovorans IT-08 beta-xylosidase / exo-xylanase (GbtXyl43B). It belongs to the glycosyl hydrolase clan F (according to carbohydrate-active enzymes database (CAZY)) which includes family 43 (GH43) and 62 (GH62) families. GH43 are inverting enzymes (i.e. they invert the stereochemistry of the anomeric carbon atom of the substrate) that have an aspartate as the catalytic general base, a glutamate as the catalytic general acid and another aspartate that is responsible for pKa modulation and orienting the catalytic acid. Many GH43 enzymes display both alpha-L-arabinofuranosidase and beta-D-xylosidase activity using aryl-glycosides as substrates. A common structural feature of GH43 enzymes is a 5-bladed beta-propeller domain that contains the catalytic acid and catalytic base. A long V-shaped groove, partially enclosed at one end, forms a single extended substrate-binding surface across the face of the propeller.
cd08980	GH43_LbAraf43-like	4.03e-57	389	654	2	275	Glycosyl hydrolase family 43 proteins such as Lactobacillus brevis alpha-L-arabinofuranosidase LbAraf43 and Geobacillus thermoleovorans GbtXyl43B. This glycosyl hydrolase family 43 (GH43) subgroup includes enzymes with beta-xylosidase (EC 3.2.1.37), alpha-L-arabinofuranosidase (EC 3.2.1.55) and possibly bifunctional xylosidase/arabinofuranosidase activities. In addition to Lactobacillus brevis alpha-L-arabinofuranosidase LbAraf43 and Geobacillus thermoleovorans IT-08 beta-xylosidase / exo-xylanase (GbtXyl43B). It belongs to the glycosyl hydrolase clan F (according to carbohydrate-active enzymes database (CAZY)) which includes family 43 (GH43) and 62 (GH62) familiesGH43 are inverting enzymes (i.e. they invert the stereochemistry of the anomeric carbon atom of the substrate) that have an aspartate as the catalytic general base, a glutamate as the catalytic general acid and another aspartate that is responsible for pKa modulation and orienting the catalytic acid. Many GH43 enzymes display both alpha-L-arabinofuranosidase and beta-D-xylosidase activity using aryl-glycosides as substrates. A common structural feature of GH43 enzymes is a 5-bladed beta-propeller domain that contains the catalytic acid and catalytic base. A long V-shaped groove, partially enclosed at one end, forms a single extended substrate-binding surface across the face of the propeller.
cd18817	GH43f_LbAraf43-like	4.18e-49	445	654	58	261	Glycosyl hydrolase family 43 such as Lactobacillus brevis alpha-L-arabinofuranosidase LbAraf43. This glycosyl hydrolase family 43 (GH43) subgroup includes characterized enzymes with alpha-L-arabinofuranosidase (EC 3.2.1.55) activity. It belongs to the glycosyl hydrolase clan F (according to carbohydrate-active enzymes database (CAZY)) which includes family 43 (GH43) and 62 (GH62) families. GH43 are inverting enzymes (i.e. they invert the stereochemistry of the anomeric carbon atom of the substrate) that have an aspartate as the catalytic general base, a glutamate as the catalytic general acid and another aspartate that is responsible for pKa modulation and orienting the catalytic acid. Many GH43 enzymes display both alpha-L-arabinofuranosidase and beta-D-xylosidase activity using aryl-glycosides as substrates. Characterized enzymes belonging to this subgroup include Lactobacillus brevis (LbAraf43) and Weissella sp (WAraf43) which show activity with similar catalytic efficiency on 1,5-alpha-L-arabinooligosaccharides with a degree of polymerization (DP) of 2-3; size is limited by an extended loop at the entrance to the active site. A common structural feature of GH43 enzymes is a 5-bladed beta-propeller domain that contains the catalytic acid and catalytic base. A long V-shaped groove, partially enclosed at one end, forms a single extended substrate-binding surface across the face of the propeller.
COG3940	COG3940	9.39e-44	383	707	9	317	Beta-xylosidase, GH43 family [Carbohydrate transport and metabolism].
pfam04616	Glyco_hydro_43	5.93e-34	386	684	9	274	Glycosyl hydrolases family 43. The glycosyl hydrolase family 43 contains members that are arabinanases. Arabinanases hydrolyze the alpha-1,5-linked L-arabinofuranoside backbone of plant cell wall arabinans. The structure of arabinanase Arb43A from Cellvibrio japonicus reveals a five-bladed beta-propeller fold. A long V-shaped groove, partially enclosed at one end, forms a single extended substrate-binding surface across the face of the propeller.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
AGB19348.1	1.17e-56	389	707	45	336
QRR01273.1	3.73e-55	383	654	33	291
AZN43459.1	2.16e-54	354	709	14	328
AYD47099.1	2.41e-54	366	712	22	348
QEH42648.1	2.81e-54	389	670	33	304

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
5M8B_A	1.87e-31	383	684	31	328	ChainA, Alpha-L-arabinofuranosidase II [Levilactobacillus brevis],5M8B_B Chain B, Alpha-L-arabinofuranosidase II [Levilactobacillus brevis]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
P82594	5.25e-23	445	654	116	318	Extracellular exo-alpha-(1->5)-L-arabinofuranosidase OS=Streptomyces chartreusis OX=1969 PE=1 SV=1

SignalP and Lipop Annotations

This protein is predicted as SP

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
0.000268	0.999105	0.000160	0.000164	0.000144	0.000141

TMHMM  Annotations     

start	end
5	27