CAZyme Information: MGYG000000528_01256

Basic Information

• Species: CAAFZY01 sp900767645
• Lineage: Bacteria; Proteobacteria; Alphaproteobacteria; RF32; CAG-239; CAAFZY01; CAAFZY01 sp900767645
• CAZyme ID: MGYG000000528_01256
• CAZy Family: GH77
• CAZyme Description: 4-alpha-glucanotransferase

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
708		81074.73	6.0001

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000000528	1493661	MAG	Fiji	Oceania

• Gene Location: Start: 25428; End: 27554 Strand: +

Full Sequence      

MSDNLNLLADKLGIARHFSDAGMHKCEYDVSDDILKFFCKEYGYKADTDEDVEQSLCRFD
NASWKKTLPAIIILRQTDMSFYAVLTATQLSQKITLSIQTGQELNFDATESAEQKEFNGK
EYRKVKITLRDCLPYGYYNLQMKYRGGIAKAVLAVVPDKCYTTDAIESGKLWGYSLQLYA
LKSKHNWGVGDFGDLKTFVDMCAKSGADVIGLNPLNVLFHDYPENASPYSSVSRLFLNPI
YIDVTAVPEFEKCDNDLLRAEIAKVKNTDLIDYTAVYNLKIKVLKNLFAILKQNAAFARQ
FADFKQEKATELDLFATFQALYSEQSKTVWGGWQAWSKELQNPASLAVAKFKAVHQEQIE
FFKFLQFEADRQLKAVYDEVKKCGLKIGLYRDLPVGVCKDSAELWADKYVFMEKSGAGAP
PDAFFTLGQKWCLGAFNPYELKNRAYEPYLKILRANMAYAGALRIDHVMGLMRLFMIPDD
KQNGTYIYYNFDDMLGLLALESHLHKCAVVGESIGNVPDGFLDKLRAHNIYSISVLWSER
WNGGYGDFKMSSDYPANVFVSVGTHDMQPLKMWWFGYDIELKRKLNMIDDNERIASYKLR
EGDRYKLLAALDFNQVWPQDKLRKADYLYGEGYPEGIEEALHKLLAKTPCKVVMLQLEDV
FEVAELQNLPGTDRETYPNWRHRLPINLEDMESSEAYWRNINAVKSAR

Enzyme Prediction     

No EC number prediction in MGYG000000528_01256.

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GH77	176	705	1.5e-136	0.9878542510121457

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
PRK11052	malQ	0.0	1	691	2	672	4-alpha-glucanotransferase; Provisional
PRK14507	PRK14507	0.0	1	708	4	721	malto-oligosyltrehalose synthase.
pfam02446	Glyco_hydro_77	2.04e-141	176	687	1	460	4-alpha-glucanotransferase. These enzymes EC:2.4.1.25 transfer a segment of a (1,4)-alpha-D-glucan to a new 4-position in an acceptor, which may be glucose or (1,4)-alpha-D-glucan.
COG1640	MalQ	8.16e-134	160	696	1	504	4-alpha-glucanotransferase [Carbohydrate transport and metabolism].
PRK14510	PRK14510	1.14e-125	168	696	721	1207	bifunctional glycogen debranching protein GlgX/4-alpha-glucanotransferase.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
AOW78810.1	1.47e-149	8	708	7	723
QKS54043.1	9.38e-142	1	695	1	715
AWU95656.1	2.81e-140	1	707	1	726
AIB11421.1	5.58e-140	1	708	1	728
ARD42991.1	2.39e-139	8	708	7	730

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
4S3P_A	3.29e-113	113	691	84	670	AmylomaltaseMalQ from Escherichia coli, apo structure [Escherichia coli K-12],4S3P_B Amylomaltase MalQ from Escherichia coli, apo structure [Escherichia coli K-12],4S3Q_A Amylomaltase MalQ from Escherichia coli in complex with maltose [Escherichia coli K-12],4S3Q_B Amylomaltase MalQ from Escherichia coli in complex with maltose [Escherichia coli K-12],4S3Q_C Amylomaltase MalQ from Escherichia coli in complex with maltose [Escherichia coli K-12]
4S3R_A	1.69e-108	113	689	84	668	AmylomaltaseMalQ from Escherichia coli in complex with the pseudo-heptasaccharide acarviosine-glucose-acarbose [Escherichia coli K-12]
5B68_A	1.39e-84	5	705	7	704	Crystalstructure of apo amylomaltase from Corynebacterium glutamicum [Corynebacterium glutamicum],5JJH_A Crystal structure of amylomaltase from Corynebacterium glutamicum [Corynebacterium glutamicum]
2X1I_A	1.06e-22	174	574	7	403	glycosidehydrolase family 77 4-alpha-glucanotransferase from thermus brockianus [Thermus brockianus]
5CPQ_A	2.63e-21	167	584	67	479	Disproportionatingenzyme 1 from Arabidopsis - apo form [Arabidopsis thaliana],5CPQ_B Disproportionating enzyme 1 from Arabidopsis - apo form [Arabidopsis thaliana],5CPS_A Disproportionating enzyme 1 from Arabidopsis - maltotriose soak [Arabidopsis thaliana],5CPS_B Disproportionating enzyme 1 from Arabidopsis - maltotriose soak [Arabidopsis thaliana],5CPT_A Disproportionating enzyme 1 from Arabidopsis - beta cyclodextrin soak [Arabidopsis thaliana],5CPT_B Disproportionating enzyme 1 from Arabidopsis - beta cyclodextrin soak [Arabidopsis thaliana],5CQ1_A Disproportionating enzyme 1 from Arabidopsis - cycloamylose soak [Arabidopsis thaliana],5CQ1_B Disproportionating enzyme 1 from Arabidopsis - cycloamylose soak [Arabidopsis thaliana],5CSU_A Disproportionating enzyme 1 from Arabidopsis - acarviostatin soak [Arabidopsis thaliana],5CSU_B Disproportionating enzyme 1 from Arabidopsis - acarviostatin soak [Arabidopsis thaliana],5CSY_A Disproportionating enzyme 1 from Arabidopsis - acarbose soak [Arabidopsis thaliana],5CSY_B Disproportionating enzyme 1 from Arabidopsis - acarbose soak [Arabidopsis thaliana]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
P45176	6.77e-118	133	696	110	685	4-alpha-glucanotransferase OS=Haemophilus influenzae (strain ATCC 51907 / DSM 11121 / KW20 / Rd) OX=71421 GN=malQ PE=3 SV=1
P15977	1.71e-112	113	691	84	670	4-alpha-glucanotransferase OS=Escherichia coli (strain K12) OX=83333 GN=malQ PE=1 SV=2
P65337	8.21e-90	1	707	4	716	4-alpha-glucanotransferase OS=Mycobacterium bovis (strain ATCC BAA-935 / AF2122/97) OX=233413 GN=malQ PE=3 SV=1
P9WK23	8.21e-90	1	707	4	716	4-alpha-glucanotransferase OS=Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) OX=83332 GN=malQ PE=1 SV=1
P9WK22	8.21e-90	1	707	4	716	4-alpha-glucanotransferase OS=Mycobacterium tuberculosis (strain CDC 1551 / Oshkosh) OX=83331 GN=malQ PE=3 SV=1

SignalP and Lipop Annotations

This protein is predicted as OTHER

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
1.000053	0.000000	0.000000	0.000000	0.000000	0.000000

TMHMM  Annotations     

There is no transmembrane helices in MGYG000000528_01256.