CAZyme Information: MGYG000000533_00082

Basic Information

• Species: Phascolarctobacterium_A sp900551335
• Lineage: Bacteria; Firmicutes_C; Negativicutes; Acidaminococcales; Acidaminococcaceae; Phascolarctobacterium_A; Phascolarctobacterium_A sp900551335
• CAZyme ID: MGYG000000533_00082
• CAZy Family: GT83
• CAZyme Description: Undecaprenyl phosphate-alpha-4-amino-4-deoxy-L-arabinose arabinosyl transferase

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
519	MGYG000000533_1|CGC1	58809.88	8.6413

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000000533	2190020	MAG	Fiji	Oceania

• Gene Location: Start: 79473; End: 81032 Strand: +

Full Sequence      

MKQIDKTSLLIVAGAALFIILFGIGGVPLLDPDEPVYAETAKEMLQTGDFLSPRIFGDYW
YDKPPMYYWLVAIAQAIFGTCEFAARLPAALMACATSVMIYVGATRLFSERAGFWSAMVL
TSCVQFFYMGKAAVTDTTLLFFMTGALLSFIHKRYWLMYICMALATVTKGPIGIVFPGTI
IFLYLIFMGQLREILRMHVIRGLLLYFLVASPWYYAMYTVHGMEFINTFLGFHNITRFTT
AEHANRVTFWYYFPVIILGMFPWTGMLVQAVKASVQDSRIDDMRNLVFLHVWALFVLLFF
TVCKTKLVSYILPMFPAMAMVIGWNIARMQAKFRHTTRFYGWTAGSGFMFVLLGVGWIIG
AEYLPEAEFSLIMLGALTLLLGAAAVFTLLYYRDVELASWLHVMIGVVTMCVAFAFVLPV
IADRFSVKTMSGVYQESCEQSTPIYVDKFLRPGFMYYAGKPGVEMVPQTNALSEALRDGS
HKYILVRGLEYRRVAKTQQIPTNITTVKEIGDIYLLEQK

Enzyme Prediction     

No EC number prediction in MGYG000000533_00082.

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GT83	3	496	1.2e-118	0.9388888888888889

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
COG1807	ArnT	5.35e-64	4	358	5	368	4-amino-4-deoxy-L-arabinose transferase or related glycosyltransferase of PMT family [Cell wall/membrane/envelope biogenesis].
PRK13279	arnT	1.17e-41	1	334	2	341	lipid IV(A) 4-amino-4-deoxy-L-arabinosyltransferase.
pfam13231	PMT_2	1.98e-17	62	215	1	159	Dolichyl-phosphate-mannose-protein mannosyltransferase. This family contains members that are not captured by pfam02366.
pfam02366	PMT	8.53e-08	37	226	35	245	Dolichyl-phosphate-mannose-protein mannosyltransferase. This is a family of Dolichyl-phosphate-mannose-protein mannosyltransferase proteins EC:2.4.1.109. These proteins are responsible for O-linked glycosylation of proteins, they catalyze the reaction:- Dolichyl phosphate D-mannose + protein <=> dolichyl phosphate + O-D-mannosyl-protein. Also in this family is Drosophila rotated abdomen protein which is a putative mannosyltransferase. This family appears to be distantly related to pfam02516 (A Bateman pers. obs.). This family also contains sequences from ArnTs (4-amino-4-deoxy-L-arabinose lipid A transferase). They catalyze the addition of 4-amino-4-deoxy-l-arabinose (l-Ara4N) to the lipid A moiety of the lipopolysaccharide. This is a critical modification enabling bacteria (e.g. Escherichia coli and Salmonella typhimurium) to resist killing by antimicrobial peptides such as polymyxins. Members such as undecaprenyl phosphate-alpha-4-amino-4-deoxy-L-arabinose arabinosyl transferase are predicted to have 12 trans-membrane regions. The N-terminal portion of these proteins is hypothesized to have a conserved glycosylation activity which is shared between distantly related oligosaccharyltransferases ArnT and PglB families.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
QTV77242.1	0.0	1	518	1	518
AOH48432.1	3.21e-165	18	496	21	504
QNH53437.1	1.83e-164	2	494	5	502
ANR70824.1	2.96e-163	2	496	5	504
AME03144.1	2.87e-158	2	496	5	504

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
5EZM_A	7.25e-34	29	332	54	370	CrystalStructure of ArnT from Cupriavidus metallidurans in the apo state [Cupriavidus metallidurans CH34],5F15_A Crystal Structure of ArnT from Cupriavidus metallidurans bound to Undecaprenyl phosphate [Cupriavidus metallidurans CH34]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
A0KGY4	7.82e-38	17	325	13	329	Undecaprenyl phosphate-alpha-4-amino-4-deoxy-L-arabinose arabinosyl transferase OS=Aeromonas hydrophila subsp. hydrophila (strain ATCC 7966 / DSM 30187 / BCRC 13018 / CCUG 14551 / JCM 1027 / KCTC 2358 / NCIMB 9240 / NCTC 8049) OX=380703 GN=arnT PE=3 SV=1
O67601	1.88e-37	6	353	2	327	Uncharacterized protein aq_1704 OS=Aquifex aeolicus (strain VF5) OX=224324 GN=aq_1704 PE=3 SV=1
A4SQX1	6.99e-37	28	323	24	327	Undecaprenyl phosphate-alpha-4-amino-4-deoxy-L-arabinose arabinosyl transferase OS=Aeromonas salmonicida (strain A449) OX=382245 GN=arnT PE=3 SV=1
O67270	9.73e-37	10	321	6	316	Uncharacterized protein aq_1220 OS=Aquifex aeolicus (strain VF5) OX=224324 GN=aq_1220 PE=3 SV=1
B2VBI7	4.20e-31	29	384	27	396	Undecaprenyl phosphate-alpha-4-amino-4-deoxy-L-arabinose arabinosyl transferase OS=Erwinia tasmaniensis (strain DSM 17950 / CFBP 7177 / CIP 109463 / NCPPB 4357 / Et1/99) OX=465817 GN=arnT PE=3 SV=1

SignalP and Lipop Annotations

This protein is predicted as OTHER

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
0.999889	0.000086	0.000012	0.000000	0.000000	0.000033

TMHMM  Annotations     

start	end
7	29
65	87
107	129
156	187
199	221
249	271
284	303
307	326
339	361
371	393
400	422