CAZyme Information: MGYG000000539_00984

Basic Information

• Species: Oribacterium sp004554245
• Lineage: Bacteria; Firmicutes_A; Clostridia; Lachnospirales; Lachnospiraceae; Oribacterium; Oribacterium sp004554245
• CAZyme ID: MGYG000000539_00984
• CAZy Family: GH36
• CAZyme Description: Bifunctional alpha-galactosidase/sucrose kinase AgaSK

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
727	MGYG000000539_15|CGC1	82699.62	5.4109

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000000539	2303957	MAG	Fiji	Oceania

• Gene Location: Start: 18763; End: 20946 Strand: +

Full Sequence      

MGITFNEEKKVFLLKTKNTSYQFRVAEFGFLEHLYYGRRVEEAVDYIPVRLRHGFEANPY
EMKQDRTLCLDVLEQEYPGFGVSDFKTSACSVIHADGSNCIDFRYVSHRIYHGKYTIDGM
PSVRNKGGDWMTLEAVLQDIATKVEVTLFYGVLESCDVITRHAVIRNLEDTAITLHAAHS
TCLSFPTDQMDQIDFYGSWGHERNAQRTPVRHGKTVVDSIRGISSHYYNPTIVLCDHEAT
ETAGDCYGVALVYSGNFQITVEVNDFKQTRVVAGIHPDTFYWKLNPGETFATPEAVMSFS
AAGLTGLSHHYHDVVRHFLIPRQFEDQRCPVLLNNWEATMIDFDEEQLMQIASAGKELGV
EMFVMDDGWFGRRTNEFRGLGDWQCNLEKLPSGIAGFSKRIHGLGLKFGIWIEPEMISED
SRLYEEHPDWCIAIPGRKPTRQRYQLILDYSRDEVVEYIFDQIYNEFKDAEIDYIKWDMN
RSMTDRFSQALPAERQGELCHRYVLGFYKLMEKLTTTFPQIRFEGCCGGGGRFDMGVLYY
APQIWCSDDTDAIERISIQHGTSFFYPPSVMGAHVSVCPNQQTGRSVSIDTRAALAYYGT
FGYELNPGELSAEEKEAVKRQIQYYQEHALTSAFGKYYRLTGVGDEFVAFMSVADDQKSA
VFSFVQLRTSANKGIICIKLRGLNESAIYTIPELGLELSGASLMYAGLPMPVIKADNTAK
TFTLLQK

Enzyme Prediction     

No EC number prediction in MGYG000000539_00984.

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GH36	11	709	4.8e-255	0.997093023255814

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
COG3345	GalA	0.0	12	716	4	673	Alpha-galactosidase [Carbohydrate transport and metabolism].
pfam02065	Melibiase	8.61e-180	289	635	1	347	Melibiase. Glycoside hydrolase families GH27, GH31 and GH36 form the glycoside hydrolase clan GH-D. Glycoside hydrolase family 36 can be split into 11 families, GH36A to GH36K. This family includes enzymes from GH36A-B and GH36D-K and from GH27.
cd14791	GH36	2.91e-135	328	627	1	299	glycosyl hydrolase family 36 (GH36). GH36 enzymes occur in prokaryotes, eukaryotes, and archaea with a wide range of hydrolytic activities, including alpha-galactosidase, alpha-N-acetylgalactosaminidase, stachyose synthase, and raffinose synthase. All GH36 enzymes cleave a terminal carbohydrate moiety from a substrate that varies considerably in size, depending on the enzyme, and may be either a starch or a glycoprotein. GH36 members are retaining enzymes that cleave their substrates via an acid/base-catalyzed, double-displacement mechanism involving a covalent glycosyl-enzyme intermediate. Two aspartic acid residues have been identified as the catalytic nucleophile and the acid/base, respectively.
pfam16875	Glyco_hydro_36N	3.45e-106	29	285	1	256	Glycosyl hydrolase family 36 N-terminal domain. This domain is found at the N-terminus of many family 36 glycoside hydrolases. It has a beta-supersandwich fold.
pfam16874	Glyco_hydro_36C	2.25e-18	648	724	2	78	Glycosyl hydrolase family 36 C-terminal domain. This domain is found at the C-terminus of many family 36 glycoside hydrolases. It has a beta-sandwich structure with a Greek key motif.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
SEU01183.1	0.0	1	727	1	729
ADL06712.1	0.0	1	725	1	727
QRV19220.1	0.0	1	725	1	727
BCK01162.1	1.86e-251	1	712	1	712
QUO31118.1	2.10e-245	1	724	1	726

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
2YFN_A	2.06e-239	1	726	1	719	galactosidasedomain of alpha-galactosidase-sucrose kinase, AgaSK [[Ruminococcus] gnavus E1],2YFO_A GALACTOSIDASE DOMAIN OF ALPHA-GALACTOSIDASE-SUCROSE KINASE, AGASK, in complex with galactose [[Ruminococcus] gnavus E1]
4FNQ_A	1.01e-215	1	716	1	716	Crystalstructure of GH36 alpha-galactosidase AgaB from Geobacillus stearothermophilus [Geobacillus stearothermophilus]
4FNR_A	4.62e-214	1	716	1	716	Crystalstructure of GH36 alpha-galactosidase AgaA from Geobacillus stearothermophilus [Geobacillus stearothermophilus],4FNR_B Crystal structure of GH36 alpha-galactosidase AgaA from Geobacillus stearothermophilus [Geobacillus stearothermophilus],4FNR_C Crystal structure of GH36 alpha-galactosidase AgaA from Geobacillus stearothermophilus [Geobacillus stearothermophilus],4FNR_D Crystal structure of GH36 alpha-galactosidase AgaA from Geobacillus stearothermophilus [Geobacillus stearothermophilus]
4FNU_A	1.49e-212	1	716	1	716	Crystalstructure of GH36 alpha-galactosidase AgaA A355E D478A from Geobacillus stearothermophilus in complex with stachyose [Geobacillus stearothermophilus],4FNU_B Crystal structure of GH36 alpha-galactosidase AgaA A355E D478A from Geobacillus stearothermophilus in complex with stachyose [Geobacillus stearothermophilus],4FNU_C Crystal structure of GH36 alpha-galactosidase AgaA A355E D478A from Geobacillus stearothermophilus in complex with stachyose [Geobacillus stearothermophilus],4FNU_D Crystal structure of GH36 alpha-galactosidase AgaA A355E D478A from Geobacillus stearothermophilus in complex with stachyose [Geobacillus stearothermophilus]
4FNT_A	2.11e-212	1	716	1	716	Crystalstructure of GH36 alpha-galactosidase AgaA A355E D548N from Geobacillus stearothermophilus in complex with raffinose [Geobacillus stearothermophilus],4FNT_B Crystal structure of GH36 alpha-galactosidase AgaA A355E D548N from Geobacillus stearothermophilus in complex with raffinose [Geobacillus stearothermophilus],4FNT_C Crystal structure of GH36 alpha-galactosidase AgaA A355E D548N from Geobacillus stearothermophilus in complex with raffinose [Geobacillus stearothermophilus],4FNT_D Crystal structure of GH36 alpha-galactosidase AgaA A355E D548N from Geobacillus stearothermophilus in complex with raffinose [Geobacillus stearothermophilus]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
G4T4R7	1.31e-235	1	726	1	719	Bifunctional alpha-galactosidase/sucrose kinase AgaSK OS=Ruminococcus gnavus OX=33038 GN=agaSK PE=1 SV=1
Q9ALJ4	2.53e-213	1	716	1	716	Alpha-galactosidase AgaA OS=Geobacillus stearothermophilus OX=1422 GN=agaA PE=1 SV=1
P43467	3.83e-197	3	722	4	725	Alpha-galactosidase 1 OS=Pediococcus pentosaceus OX=1255 GN=agaR PE=3 SV=1
G1UB44	2.37e-195	3	722	6	726	Alpha-galactosidase Mel36A OS=Lactobacillus acidophilus (strain ATCC 700396 / NCK56 / N2 / NCFM) OX=272621 GN=melA PE=1 SV=1
P27756	1.23e-162	53	727	51	720	Alpha-galactosidase OS=Streptococcus mutans serotype c (strain ATCC 700610 / UA159) OX=210007 GN=aga PE=3 SV=3

SignalP and Lipop Annotations

This protein is predicted as OTHER

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
1.000059	0.000000	0.000000	0.000000	0.000000	0.000000

TMHMM  Annotations     

There is no transmembrane helices in MGYG000000539_00984.