CAZyme Information: MGYG000000541_01062

Basic Information

• Species: Bruticola sp004554425
• Lineage: Bacteria; Eremiobacterota; Xenobia; Xenobiales; Xenobiaceae; Bruticola; Bruticola sp004554425
• CAZyme ID: MGYG000000541_01062
• CAZy Family: GT51
• CAZyme Description: Penicillin-binding protein 2D

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
701		76162.33	5.6207

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000000541	2312271	MAG	Fiji	Oceania

• Gene Location: Start: 3588; End: 5693 Strand: -

Full Sequence      

MRNAILAIEDSRFYEHHGVDPVGVLRAAVDLYRSGGQVHQGASTITMQLARAFFLNDDLS
FHRKIREALLALQIEKSFSKDEILELYLNHVYLGAGAYGVHAAANVYFLTTPDKLTPVQA
AIIAGLPASPSYYSPLVDERAAASRALEVLGRMRQLDMITQKQYREAVDTLRKGIKYANK
GRTEFQVLKVPYFTTYVLKELNNRYSEDMLYRGGLKIYTTVDLKLQEKAEKIVAEKIAQN
KEWYNASTAAAVVVENSTGYIRAMVGGSGWSKDNQFNRAWQARRQPGSCFKVFVYTTAVE
SGYGPDTIISDNPTTYKISDTETWSPKNSDGKFLGNIPMLRALMGSRNVAAVKLLTMVGP
ERVIEYAYRMGIKERLEPNLSLALGSAVVTPLEMACAFTCFPNGGIKVQPSGIKMVYDND
GNVIEDNTFPRQEEVLSESTAYTMCSMLKSAVEGGTGYGAQIPGRAVGGKTGTTDDYRDG
WFVGFTPQYTCAVWAGNDDYTSMWRGYGGDVAAPIWKAVMSYAVKDLPNKQFEKPKSGLV
SVLMCSESKCRAGAKCMSTYKEYFRPGEVPNKFCPIHGGAASVPSIAAPKDDGCGTKKED
QSQAAPSQTETEPTPVPADAPQTWGEIVPDVSTEHSESVTPDLTPENIPIPNTVYAPDTA
APPPVSAPAPEAPAIAPPEPQAVPIGGTSEAAGAPAEAPVE

Enzyme Prediction     

EC	2.4.1.129

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GT51	1	153	5.8e-56	0.8531073446327684

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
COG5009	MrcA	0.0	1	567	79	761	Membrane carboxypeptidase/penicillin-binding protein [Cell wall/membrane/envelope biogenesis].
COG0744	MrcB	0.0	1	536	88	609	Membrane carboxypeptidase (penicillin-binding protein) [Cell wall/membrane/envelope biogenesis].
TIGR02074	PBP_1a_fam	0.0	2	526	15	531	penicillin-binding protein, 1A family. Bacterial that synthesize a cell wall of peptidoglycan (murein) generally have several transglycosylases and transpeptidases for the task. This family consists of bifunctional transglycosylase/transpeptidase penicillin-binding proteins (PBP). In the Proteobacteria, this family includes PBP 1A but not the paralogous PBP 1B (TIGR02071). This family also includes related proteins, often designated PBP 1A, from other bacterial lineages. [Cell envelope, Biosynthesis and degradation of murein sacculus and peptidoglycan]
COG4953	PbpC	4.38e-108	4	566	73	602	Membrane carboxypeptidase/penicillin-binding protein PbpC [Cell wall/membrane/envelope biogenesis].
PRK11636	mrcA	2.29e-100	1	570	78	828	penicillin-binding protein 1a; Provisional

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
ADM95041.1	1.27e-141	3	574	95	646
ADM94922.1	1.68e-141	3	589	94	656
QQB17780.1	1.44e-135	1	542	98	632
SNU98291.1	1.44e-135	1	542	98	632
ACZ24745.1	1.44e-135	1	542	98	632

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
4OON_A	3.40e-97	3	593	53	789	Crystalstructure of PBP1a in complex with compound 17 ((4Z,8S,11E,14S)-5-(2-amino-1,3-thiazol-4-yl)-14-(5,6-dihydroxy-1,3-dioxo-1,3-dihydro-2H-isoindol-2-yl)-8-formyl-2-methyl-6-oxo-3,10-dioxa-4,7,11-triazatetradeca-4,11-diene-2,12,14-tricarboxylic acid) [Pseudomonas aeruginosa PAO1]
3UDF_A	1.20e-85	3	528	53	720	ChainA, Penicillin-binding protein 1a [Acinetobacter baumannii],3UDF_B Chain B, Penicillin-binding protein 1a [Acinetobacter baumannii],3UDI_A Chain A, Penicillin-binding protein 1a [Acinetobacter baumannii],3UDI_B Chain B, Penicillin-binding protein 1a [Acinetobacter baumannii],3UDX_A Chain A, Penicillin-binding protein 1a [Acinetobacter baumannii],3UDX_B Chain B, Penicillin-binding protein 1a [Acinetobacter baumannii],3UE0_A Chain A, Penicillin-binding protein 1a [Acinetobacter baumannii],3UE0_B Chain B, Penicillin-binding protein 1a [Acinetobacter baumannii],3UE1_A Chain A, Penicillin-binding protein 1a [Acinetobacter baumannii],3UE1_B Chain B, Penicillin-binding protein 1a [Acinetobacter baumannii]
5FGZ_A	3.56e-68	3	521	170	691	E.coli PBP1b in complex with FPI-1465 [Escherichia coli K-12],5HL9_A E. coli PBP1b in complex with acyl-ampicillin and moenomycin [Escherichia coli K-12],5HLA_A E. coli PBP1b in complex with acyl-cephalexin and moenomycin [Escherichia coli K-12],5HLB_A E. coli PBP1b in complex with acyl-aztreonam and moenomycin [Escherichia coli K-12],5HLD_A E. coli PBP1b in complex with acyl-CENTA and moenomycin [Escherichia coli K-12],6YN0_A Structure of E. coli PBP1b with a FtsN peptide activating transglycosylase activity [Escherichia coli K-12],7LQ6_A Chain A, Penicillin-binding protein 1B [Escherichia coli K-12]
3VMA_A	4.97e-68	3	521	191	712	CrystalStructure of the Full-Length Transglycosylase PBP1b from Escherichia coli [Escherichia coli K-12]
5U2G_A	9.15e-67	3	535	54	769	2.6Angstrom Resolution Crystal Structure of Penicillin-Binding Protein 1A from Haemophilus influenzae [Haemophilus influenzae Rd KW20],5U2G_B 2.6 Angstrom Resolution Crystal Structure of Penicillin-Binding Protein 1A from Haemophilus influenzae [Haemophilus influenzae Rd KW20]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
O66874	2.75e-104	3	540	77	684	Penicillin-binding protein 1A OS=Aquifex aeolicus (strain VF5) OX=224324 GN=mrcA PE=1 SV=1
Q07806	3.30e-96	3	593	80	816	Penicillin-binding protein 1A OS=Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1) OX=208964 GN=mrcA PE=1 SV=2
Q5FAC7	5.40e-96	1	604	80	770	Penicillin-binding protein 1A OS=Neisseria gonorrhoeae (strain ATCC 700825 / FA 1090) OX=242231 GN=mrcA PE=3 SV=3
O05131	5.40e-96	1	604	80	770	Penicillin-binding protein 1A OS=Neisseria gonorrhoeae OX=485 GN=mrcA PE=1 SV=3
O87579	7.54e-96	1	535	80	717	Penicillin-binding protein 1A OS=Neisseria lactamica OX=486 GN=mrcA PE=3 SV=1

SignalP and Lipop Annotations

This protein is predicted as OTHER

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
1.000077	0.000001	0.000000	0.000000	0.000000	0.000000

TMHMM  Annotations     

There is no transmembrane helices in MGYG000000541_01062.