CAZyme Information: MGYG000000541_01701

Basic Information

• Species: Bruticola sp004554425
• Lineage: Bacteria; Eremiobacterota; Xenobia; Xenobiales; Xenobiaceae; Bruticola; Bruticola sp004554425
• CAZyme ID: MGYG000000541_01701
• CAZy Family: GT51
• CAZyme Description: Monofunctional biosynthetic peptidoglycan transglycosylase

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
744		83849.13	9.1375

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000000541	2312271	MAG	Fiji	Oceania

• Gene Location: Start: 280; End: 2514 Strand: -

Full Sequence      

MQDAVLTAEDRRFYYHCGADPLAIMRSAYYNCKVGRVIMGGSTISEQLIRLLMPARPRTL
KNKFIELLYSLRLSFNFSKEQILEAYLNRVYFGGQAYGLEAAAQLYFGCPARALSPAQGA
FLTVLVRSPQELAPYKDINPALKLQRDLLLSMEKRGILGSADLKRALAEKITLAPLSERF
AAPHFCDFVVAQNEQWPAQDAVRIDTTLDLKIQDYAENILNYHLERLASHNVKNGSLVVL
DIKRGDILAMVGSKNYFSLPDGQNNGSLTLRQPGSALKPFTYALALQHGFTAASILPDLS
YLGAMLKDAFVPENYDRQFHGPVRLRQALACSYNVPAVYVLQKIGINSLLELLHNLGFTE
LNKNAQHYGLGLTLGSGEVNLLHLANAYRGLASGGKYQAVRFCQAYTDSRGEKHFWPLAN
TKQKVISPEVCTLIGDILADSRARSEAFGYHSALNLPFRCSVKTGTSKDYRDNWCIGYDD
NYVVGVWTGNFDASAMHNISGITGAAPIFRDVMKYVHQYRSRSSKILNIGKFNETEPGLE
KRWICAESGAAPGPYCYNKIQEYFLFNTFPHEVCQVHKLYCFSKTNKQPVNPLSCSPYEY
FTKVVPVYPPLYRAWMREHKMLIPPQWTLYRKIDSKAQKKHAQSSRLSMQDESEPPEDKI
IFPEDGAVFRIDPFLKRQHQNVNLRATSSAPNKKLTWKIDGKIFQVSSEPHVVSWPLREG
VHRITLQTEDSKSSVDSITVTVYP

Enzyme Prediction     

No EC number prediction in MGYG000000541_01701.

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GT51	1	149	1.3e-45	0.8361581920903954

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
TIGR02073	PBP_1c	1.24e-168	1	742	45	726	penicillin-binding protein 1C. This subfamily of the penicillin binding proteins includes the member from E. coli designated penicillin-binding protein 1C. Members have both transglycosylase and transpeptidase domains and are involved in forming cross-links in the late stages of peptidoglycan biosynthesis. All members of this subfamily are presumed to have the same basic function. [Cell envelope, Biosynthesis and degradation of murein sacculus and peptidoglycan]
COG4953	PbpC	4.98e-137	2	734	71	725	Membrane carboxypeptidase/penicillin-binding protein PbpC [Cell wall/membrane/envelope biogenesis].
TIGR02074	PBP_1a_fam	3.83e-133	2	519	15	531	penicillin-binding protein, 1A family. Bacterial that synthesize a cell wall of peptidoglycan (murein) generally have several transglycosylases and transpeptidases for the task. This family consists of bifunctional transglycosylase/transpeptidase penicillin-binding proteins (PBP). In the Proteobacteria, this family includes PBP 1A but not the paralogous PBP 1B (TIGR02071). This family also includes related proteins, often designated PBP 1A, from other bacterial lineages. [Cell envelope, Biosynthesis and degradation of murein sacculus and peptidoglycan]
COG0744	MrcB	2.63e-127	1	555	88	628	Membrane carboxypeptidase (penicillin-binding protein) [Cell wall/membrane/envelope biogenesis].
COG5009	MrcA	1.54e-90	1	577	79	771	Membrane carboxypeptidase/penicillin-binding protein [Cell wall/membrane/envelope biogenesis].

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
QXD15424.1	5.37e-163	4	726	83	776
ARA92794.1	1.03e-153	4	731	105	802
ACC98391.1	4.66e-143	4	732	80	709
QED34039.1	4.76e-141	1	724	74	782
QDG52817.1	4.76e-141	1	724	74	782

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
3ZG8_B	2.90e-40	77	520	7	464	CrystalStructure of Penicillin Binding Protein 4 from Listeria monocytogenes in the Ampicillin bound form [Listeria monocytogenes],3ZG9_B Crystal Structure of Penicillin-Binding Protein 4 from Listeria monocytogenes in the Cefuroxime bound form [Listeria monocytogenes],3ZGA_B Crystal Structure of Penicillin-Binding Protein 4 from Listeria monocytogenes in the Carbenicillin bound form [Listeria monocytogenes]
3ZG7_B	9.88e-37	77	505	7	447	CrystalStructure of Penicillin-Binding Protein 4 from Listeria monocytogenes in the apo form [Listeria monocytogenes]
4OON_A	8.64e-35	4	489	54	694	Crystalstructure of PBP1a in complex with compound 17 ((4Z,8S,11E,14S)-5-(2-amino-1,3-thiazol-4-yl)-14-(5,6-dihydroxy-1,3-dioxo-1,3-dihydro-2H-isoindol-2-yl)-8-formyl-2-methyl-6-oxo-3,10-dioxa-4,7,11-triazatetradeca-4,11-diene-2,12,14-tricarboxylic acid) [Pseudomonas aeruginosa PAO1]
5FGZ_A	5.27e-33	1	510	168	687	E.coli PBP1b in complex with FPI-1465 [Escherichia coli K-12],5HL9_A E. coli PBP1b in complex with acyl-ampicillin and moenomycin [Escherichia coli K-12],5HLA_A E. coli PBP1b in complex with acyl-cephalexin and moenomycin [Escherichia coli K-12],5HLB_A E. coli PBP1b in complex with acyl-aztreonam and moenomycin [Escherichia coli K-12],5HLD_A E. coli PBP1b in complex with acyl-CENTA and moenomycin [Escherichia coli K-12],6YN0_A Structure of E. coli PBP1b with a FtsN peptide activating transglycosylase activity [Escherichia coli K-12],7LQ6_A Chain A, Penicillin-binding protein 1B [Escherichia coli K-12]
3VMA_A	5.67e-33	1	510	189	708	CrystalStructure of the Full-Length Transglycosylase PBP1b from Escherichia coli [Escherichia coli K-12]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
O66874	2.36e-59	3	516	77	667	Penicillin-binding protein 1A OS=Aquifex aeolicus (strain VF5) OX=224324 GN=mrcA PE=1 SV=1
P70997	3.61e-59	4	574	89	657	Penicillin-binding protein 2D OS=Bacillus subtilis (strain 168) OX=224308 GN=pbpG PE=2 SV=3
P76577	1.63e-56	3	512	78	560	Penicillin-binding protein 1C OS=Escherichia coli (strain K12) OX=83333 GN=pbpC PE=1 SV=1
Q9KUC0	6.37e-54	1	515	192	713	Penicillin-binding protein 1B OS=Vibrio cholerae serotype O1 (strain ATCC 39315 / El Tor Inaba N16961) OX=243277 GN=mrcB PE=3 SV=1
P40750	7.14e-51	1	514	88	621	Penicillin-binding protein 4 OS=Bacillus subtilis (strain 168) OX=224308 GN=pbpD PE=1 SV=2

SignalP and Lipop Annotations

This protein is predicted as OTHER

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
0.999900	0.000120	0.000007	0.000000	0.000000	0.000007

TMHMM  Annotations     

There is no transmembrane helices in MGYG000000541_01701.