CAZyme Information: MGYG000000548_01686

Basic Information

• Species: Sutterella sp900770335
• Lineage: Bacteria; Proteobacteria; Gammaproteobacteria; Burkholderiales; Burkholderiaceae; Sutterella; Sutterella sp900770335
• CAZyme ID: MGYG000000548_01686
• CAZy Family: GH102
• CAZyme Description: Membrane-bound lytic murein transglycosylase A

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
413		45181.47	10.1112

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000000548	2283169	MAG	Fiji	Oceania

• Gene Location: Start: 43; End: 1284 Strand: -

Full Sequence      

MLRPVRNSARQQILNPRNTLKGLAKPLRLAALSLLLALTACTTVETPEPGRPVPDARFSA
EPWERVPAAEPQAWSNALSAFRLSCARMGGSAPWSELCAEAAATPAESAESFFRARFTPW
RIEASSESANGVEWRDTGLMTGYYEPLLHGSRTKGGKYVHPIYGVPDDLLIIDLASLHPE
LKGKRLRGRLDGRRVVPYGDRAAITKRTDMDRWALAWVDDPVDAFFLQIQGSGRIALPDG
SFMRVGFADQNGWPYKAIGKWLSEHEGIPMHALSMQRIRAWARANPSRTDEALAQNPSFV
FFEERSGDPALGPLGAQGAPLTPRASVAADPRKWRLGTPFLIEASQDRPALSFVRPVVAQ
DTGGAIQGVLRFDFFWGFGDEAGAAAGRQKSRVRAWALVPNGLAPEAIRPGAR

Enzyme Prediction     

No EC number prediction in MGYG000000548_01686.

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GH102	148	303	2.2e-56	0.9936305732484076

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
COG2821	MltA	3.56e-92	65	407	34	372	Membrane-bound lytic murein transglycosylase [Cell wall/membrane/envelope biogenesis].
pfam03562	MltA	1.96e-90	77	302	9	230	MltA specific insert domain. This beta barrel domain is found inserted in the MltA a murein degrading transglycosylase enzyme. This domain may be involved in peptidoglycan binding.
cd14668	mlta_B	7.02e-72	148	305	1	159	Domain B insert of mltA_like lytic transglycosylases. Escherichia coli MltA is a membrane-bound lytic transglycosylase comprised of two domains separated by a large groove, where the peptidoglycan strand binds. Domain A is made up of an N-terminal and a C-terminal portion, which correspond to the 3D domain, named for 3 conserved aspartate residues. Domain B is inserted within the linear sequence of domain A. MltA is distinct from other bacterial lytic transglycosylases (LTs), which are similar to each other. Escherichia coli peptidoglycan lytic transglycosylase (LT) initiates cell wall recycling in response to damage, during bacterial fission, and cleaves peptidoglycan (PG) to create functional spaces in its wall. PG chains (also known as murein), the major components of the bacterial cell wall, are comprised of alternating beta-1-4-linked N-acetylmuramic acid (MurNAc) and N-acetyl-D-glucosamine (GlcNAc), and lytic transglycosylases cleave this beta-1-4 bond. Typically, peptidoglycan lytic transglycosylases (LT) are exolytic, releasing Metabolite 1 (GlcNAc-anhMurNAc-L-Ala-D-Glu-m-Dap-D-Ala-D-Ala) from the ends of the PG strands. In contrast, MltE is endolytic , cleaving in the middle of PG strands, with further processing to Metabolite 1 accomplished by other LTs. In E. coli, there are six membrane-bound LTs: MltA-MltF and soluble Slt70. Slt35 is a soluble fragment cleaved from MltB. Bacterial LTs are classified in 4 families: Family 1 includes slt70 MltC-MltF, Family 2 includes MltA, Family 3 includes MltB, and Family 4 of bacteriophage origin. While most of the LT family members are similar in structure and sequence with a lysozyme-like fold, Family 2 (including mltA) is distinct.
smart00925	MltA	1.26e-64	147	302	1	152	MltA specific insert domain. This beta barrel domain is found inserted in the MltA a murein degrading transglycosylase enzyme. This domain may be involved in peptidoglycan binding.
PRK11162	mltA	3.10e-49	139	398	117	354	murein transglycosylase A; Provisional

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
QDA55021.1	1.42e-189	14	411	5	397
BBF22454.1	2.25e-172	25	409	4	394
QQS89395.1	2.36e-162	58	410	47	398
ANU66434.1	4.76e-136	30	409	12	400
QQQ97583.1	4.76e-136	30	409	12	400

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
7ESJ_A	5.01e-85	42	403	9	362	ChainA, membrane-bound lytic murein transglycosylase A [Acinetobacter baumannii],7ESJ_B Chain B, membrane-bound lytic murein transglycosylase A [Acinetobacter baumannii]
6QK4_B	6.35e-78	56	401	23	347	Lytictransglycosylase, LtgG, of Burkholderia pseudomallei. [Burkholderia pseudomallei]
2G5D_A	3.28e-75	56	406	40	419	Crystalstructure of MltA from Neisseria gonorrhoeae Monoclinic form [Neisseria gonorrhoeae FA 1090]
2G6G_A	2.19e-72	56	406	40	419	Crystalstructure of MltA from Neisseria gonorrhoeae [Neisseria gonorrhoeae FA 1090]
3CZB_A	8.38e-47	77	401	26	342	Crystalstructure of putative transglycosylase from Caulobacter crescentus [Caulobacter vibrioides CB15],3CZB_B Crystal structure of putative transglycosylase from Caulobacter crescentus [Caulobacter vibrioides CB15]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
P0A935	6.48e-30	120	398	105	353	Membrane-bound lytic murein transglycosylase A OS=Escherichia coli (strain K12) OX=83333 GN=mltA PE=1 SV=1
P0A936	6.48e-30	120	398	105	353	Membrane-bound lytic murein transglycosylase A OS=Escherichia coli O157:H7 OX=83334 GN=mltA PE=3 SV=1
Q9KPQ4	4.19e-24	139	398	112	349	Membrane-bound lytic murein transglycosylase A OS=Vibrio cholerae serotype O1 (strain ATCC 39315 / El Tor Inaba N16961) OX=243277 GN=mltA PE=3 SV=1
Q8K9A7	1.72e-22	131	398	117	364	Membrane-bound lytic murein transglycosylase A homolog OS=Buchnera aphidicola subsp. Schizaphis graminum (strain Sg) OX=198804 GN=mltA PE=3 SV=1
P57531	5.54e-20	140	398	118	356	Membrane-bound lytic murein transglycosylase A homolog OS=Buchnera aphidicola subsp. Acyrthosiphon pisum (strain APS) OX=107806 GN=mltA PE=3 SV=1

SignalP and Lipop Annotations

This protein is predicted as LIPO

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
0.001634	0.354517	0.643699	0.000119	0.000036	0.000011

TMHMM  Annotations     

There is no transmembrane helices in MGYG000000548_01686.